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Introducing Gamborg’s B5, a high-potential medium for isolated microspore culture, and presenting a new MS medium-based protocol for androgenic plant regeneration in eggplant (Solanum melongena L.)
The androgenesis is efficiently used to produce doubled-haploid plants. Currently, the modified NLN medium is the only suggested medium for...
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Employing active learning in the optimization of culture medium for mammalian cells
Medium optimization is a crucial step during cell culture for biopharmaceutics and regenerative medicine; however, this step remains challenging, as...
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Basic Fibroblast Growth Factor Accumulation in Culture Medium Masks the Direct Antitumor Effect of Anti-VEGF Agent Bevacizumab
AbstractThe direct antitumor effect of bevacizumab (BEV) has long been debated. Evidence of the direct antitumor activities of drugs are mainly...
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Media matters: culture medium-dependent hypervariable phenotype of mesenchymal stromal cells
BackgroundDespite a long history of investigation and sustained efforts in clinical testing, the number of market authorisations for mesenchymal...
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Culture of Mouse Thymic Epithelial Cells in Serum-Free Medium
Primary cell culture systems are widely used as a valuable method for analyzing the biological functions of specific cells in vitro. Recently,...
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An untargeted metabolomics approach to study changes of the medium during human cornea culture
IntroductionTwo main approaches (organ culture and hypothermia) for the preservation and storage of human donor corneas are globally adopted for...
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Buffer-free CAS assay using a diluted growth medium efficiently detects siderophore production and microbial growth
Siderophores are iron chelators and low-molecular-weight compounds secreted by various microorganisms under low-iron conditions. Many microorganisms...
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Short-term adaptation as a tool to improve bioethanol production using grass press-juice as fermentation medium
AbstractGrass raw materials collected from grasslands cover more than 30% of Europe’s agricultural area. They are considered very attractive for the...
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A review of electronic devices for tracking small and medium migratory shorebirds
Small- to medium-sized shorebirds (< 200 g) can be particularly difficult to track with electronic devices due to their small size and long-distance...
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Development of a standard medium for culturing the termite Reticulitermes speratus
Termites are eusocial insects that play an important role as decomposers in the natural environment. Therefore, they attract the attention of many...
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Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources
Production of medium chain length polyhydroxyalkanoate (mcl-PHA) was attempted using Pseudomonas gessardii NIBRBAC000509957, which was isolated from...
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Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro
ObjectivesThis study assessed possible osteogenic differentiation caused by electromagnetic fields (EMF) on rat bone-marrow-derived stem cells...
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Personalised modelling of clinical heterogeneity between medium-chain acyl-CoA dehydrogenase patients
BackgroundMonogenetic inborn errors of metabolism cause a wide phenotypic heterogeneity that may even differ between family members carrying the same...
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Protein Profile of the Incubation Medium and Extract of Cestodes from the Intestines of Different Fish Species
AbstractThe protein composition of the incubation medium and extracts of different species of cestodes inhabiting the intestines of freshwater fish...
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Screening and optimisation of in vitro pollen germination medium for sweetpotato (Ipomoea batatas)
BackgroundSweetpotato is an important vegetable and food crop that is bred through sexual crosses and systematic selection. The use of in vitro...
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Spectral and conformational characteristics of phycocyanin associated with changes of medium pH
C-phycocyanin (C-PC) is the main component of water-soluble light-harvesting complexes (phycobilisomes, PBS) of cyanobacteria. PBS are involved in...
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Improvement of ribonucleic acid production in Cyberlindnera jadinii and optimization of fermentation medium
To enhance the ribonucleic acid (RNA) productivity for industrial applications, this study employed strain screening and medium optimization to...
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Significant improvement of bone marrow-derived MSC expansion from MDS patients by defined xeno-free medium
BackgroundRobust and reliable in vitro and in vivo models of primary cells are necessary to study the pathomechanisms of Myelodysplastic Neoplasms...
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High-cell-density cultivation of Vibrio natriegens in a low-chloride chemically defined medium
AbstractVibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has...
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Short- and medium-term impacts of small-scale vegetable support on food security: evidence from Syria
Small-scale agricultural and horticultural interventions play a critical role in improving nutrition and food security of vulnerable households in...