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Somatic Embryogenesis in Catharanthus Roseus: Proteomics of Embryogenic and Non-embryogenic Tissues; and Genome Size Analysis of Regenerated Plant
The asexual, non-zygotic (somatic) embryogenesis was reported over 60 years ago. It is a unique process in which somatic cell transforms into embryo...
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Microbial transformation of water-insoluble substrates by two types of novel interface bioprocesses, tacky liquid–liquid interface bioreactor and non-aqueous sporular bioconversion system
Although microbial transformation has been expected as a substitution technology for organic synthesis, microbial toxicity and water-insolubility of...
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Effects of a Laparotomy on Targeted Lipidomics Profiles in a Mouse Model of Surgical Stress During Aging
Laparotomy (EL) is one of the most common procedures performed among surgical specialties. Previous research demonstrates that surgery is associated...
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Improvement of the conjugation transfer of N. gerenzanensis based on the synergistic effect of quorum sensing and antibiotic interference
Nonomuraea gerenzanensis ( N. gerenzanensis ) is known for its ability to biosynthesize A40926, the precursor of the glycopeptide antibiotic (GPA)...
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Fine-scale metabolic discontinuity in a stratified prokaryote microbiome of a Red Sea deep halocline
Deep-sea hypersaline anoxic basins are polyextreme environments in the ocean’s interior characterized by the high density of brines that prevents...
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Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging
Cotton fibers are extremely elongated single cells and have long been regarded as an ideal model to investigate polarized plant cell elongation....
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Leveraging molecular docking to understand Congo red degradation by Staphylococcus caprae MB400
Congo red (CR) is a genotoxic, sulphonated azo dye and poses significant pollution problem. We hereby report its degradation by Staphylococcus caprae ...
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Protein fibril length in cerebrospinal fluid is increased in Alzheimer’s disease
Alzheimer’s disease (AD) associated proteins exist in cerebrospinal fluid (CSF). This paper evidences that protein aggregate morphology distinctly...
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Interactions of Tissue-Resident T Cells
Resident memory T cells (TRM) are non-circulating cells that play a critical role in protection from local infections and cancers. Flow cytometric...
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Three-Dimensional Hanging Drop Spheroid Plates for Easy Chimeric Antigen Receptor (CAR) T Cytotoxicity Assay
Chimeric antigen receptor (CAR) T cell therapy shows a highly effective therapeutic effect on B-cell malignancies. The tumor microenvironment (TME)...
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A New Producer of Echinocandins, Ascomycete Coleophoma sp. Isolated from the Lichen Stereocaulon paschale
AbstractFor the first time, phytopathogenic fungi of the genus Coleophoma were detected in the lichen genus Stereocaulon paschale (L.) Hoffm. On...
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Isolation of Human Villous Cytotrophoblastic Cells from Term Placenta for Transfection
Isolation of villous cytotrophoblastic cells (vCTBs) from placental tissue provides the possibility of studying differentiation of these cells as...
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Occurrence and relevance of Mycoplasma sturni in free ranging barn swallows (Hirundo rustica) in Germany
In poultry industry, mycoplasmas cause large economic losses. The pathogenic significance of mycoplasmas varies widely between species. In birds...
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Auxin-mediated root branching is determined by the form of available nitrogen
To improve water and nutrient acquisition from the soil, plants can modulate their root system architecture. Despite the importance of changes in...
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Physiological Polyphosphate: A New Molecular Paradigm in Biomedical and Biocomputational Applications for Human Therapy
Inorganic polyphosphates (polyP) consist of linear chains of orthophosphate units linked together by high-energy phosphoanhydride bonds. The family...
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Type I Lipoteichoic Acid (LTA) Detection by Western Blot
Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria including Staphylococcus...
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In Situ Hybridization as a Method to Examine Gene Regulatory Activity In Vivo
Transcription factor-enhancer binding events are among the most well-studied protein-DNA interactions, allowing researchers to determine mechanisms...
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Centrifugation
The technique of separating substances under the influence of centrifugal force is termed as centrifugation. Particles get separated from the...
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Heterologous Production of Plant Terpenes in the Photosynthetic Bacterium Rhodobacter capsulatus
Terpenes are one of the largest classes of secondary metabolites that occur in all kingdoms of life and offer diverse valuable properties for food...