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Laser Capture Microdissection: From Genomes to Chromosomes, from Complex Tissue to Single-Cell Analysis
Laser microdissection (LM) is a powerful tool for various molecular analyses providing pure samples for genomic, transcriptomic, and proteomic...
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Laser capture microdissection transcriptome (LCM RNA-seq) reveals BcDFR is a key gene in anthocyanin synthesis of non-heading Chinese cabbage
BackgroundPurple non-heading Chinese cabbage [ Brassica campestris (syn. Brassica rapa ) ssp. chinensis ] has become popular because of its richness in...
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Shape decomposition algorithms for laser capture microdissection
BackgroundIn the context of biomarker discovery and molecular characterization of diseases, laser capture microdissection is a highly effective...
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Laser Capture Microdissection of Mouse Growth Plate Cartilage
The ability to identify, isolate, and study pure populations of cells is critical for understanding normal physiology in organs and tissues, which...
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RNA Isolation from Nematode-Induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection
Nematodes are diverse multicellular organisms that are most abundantly found in the soil. Most nematodes are free-living and feed on a range of...
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Disseminated Cryptococcosis Presenting as Cellulitis Diagnosed by Laser Capture Microdissection: A Case Report and Literature Review
Disseminated cryptococcosis primarily affects immunosuppressed patients and has a poor outcome if diagnosis and treatment are delayed. Skin lesions...
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Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing
Our understanding of nerve regeneration can be enhanced by delineating its underlying molecular activities at single-neuron resolution in model...
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Laser Capture Microdissection –Based mRNA Expression Microarrays and Single-Cell RNA Sequencing in Atherosclerosis Research
A major goal of methodologies related to large scale gene expression analyses is to initiate comprehensive information on transcript signatures in...
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Neoplastic cell enrichment of tumor tissues using coring and laser microdissection for proteomic and genomic analyses of pancreatic ductal adenocarcinoma
BackgroundThe identification of differentially expressed tumor-associated proteins and genomic alterations driving neoplasia is critical in the...
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Spatiotemporal plant hormone analysis from cryosections using laser microdissection-liquid chromatography-mass spectrometry
Laser microdissection (LMD) is used for isolating specific regions or single cells from a wide variety of tissue samples under direct microscopic...
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Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method
The success of single cell type-specific gene expression or functional study largely depends on the efficient isolation of high-quality RNA from...
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Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome of Cellular Protrusions
Laser capture microdissection (LCM) provides a fast, specific, and versatile method to isolate and enrich cells in mixed populations and/or...
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Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection
Testicular germ cell tumors are among the most common malignancies seen in children and young adults. Genomic studies have identified characteristic...
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Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning
Laser capture microdissection (LCM) has become a powerful technique that allows analyzing gene expression in specific target cells from complex...
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Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing
Somatic mutations accumulate in healthy tissues as we age, giving rise to cancer and potentially contributing to ageing. To study somatic mutations...
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Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Laser capture microdissection is a valuable technique in individually isolating single cells whether in tissue networks or deposited from a cell...
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The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow
Reversible protein phosphorylation represents a key mechanism by which signals are transduced in eukaryotic cells. Dysregulated phosphorylation is...
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Laser capture microdissection coupled mass spectrometry (LCM-MS) for spatially resolved analysis of formalin-fixed and stained human lung tissues
BackgroundHaematoxylin and eosin (H&E)—which respectively stain nuclei blue and other cellular and stromal material pink—are routinely used for...
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Effect of dynamic exclusion and the use of FAIMS, DIA and MALDI-mass spectrometry imaging with ion mobility on amyloid protein identification
Amyloidosis is a disease characterized by local and systemic extracellular deposition of amyloid protein fibrils where its excessive accumulation in...
