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Mass spectrometry analysis of the photosystem II assembly factor Psb27 revealed variations in its lipid modification
The assembly of large, multi-cofactor membrane protein complexes like photosystem II (PSII) requires a high level of coordination. The process is...
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Remembering James Barber (1940–2020)
James Barber, known to colleagues and friends as Jim, passed away in January 2020 after a long battle against cancer. During his long and...
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Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry...
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Physiological and evolutionary implications of tetrameric photosystem I in cyanobacteria
Photosystem I (PSI) is present as trimeric complexes in most characterized cyanobacteria and as monomers in plants and algae. Recent reports of...
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Full Membrane Protein Coverage Digestion and Quantitative Bottom-Up Mass Spectrometry Proteomics
A true and accurate bottom-up global proteomic measurement will only be achieved when all proteins in a sample can be digested efficiently and at... -
Säubern und Putzen
Das Säubern von Proteinen gehört zum Biochemiker wie das Rohre putzen zum Klempner und zu beidem gehören Fertigkeiten und Werkzeuge. Die... -
Proteogenomic review of the changes in primate apoC-I during evolution
Apolipoprotein C-I has evolved more rapidly than any of the other soluble apolipoproteins. During the course of primate evolution, the gene for this...
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Phenotypic and Genomic Analysis of Hypervirulent Human-associated Bordetella bronchiseptica
BackgroundB. bronchiseptica infections are usually associated with wild or domesticated animals, but infrequently with humans. A recent phylogenetic...
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Information and Data Sharing Policy, Genomic Science Program, DOE
The Office of Biological and Environmental Research (OBER) will require that all publishable information resulting from GTL funded research must...
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Hydrophobic Proteome Analysis of Triple Negative and Hormone-Receptor-Positive-Her2-Negative Breast Cancer by Mass Spectrometer
IntroductionIt is widely believed that discovery of specific, sensitive, and reliable tumor biomarkers can improve the treatment of cancer....
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A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells
Orai1 and STIM1 are critical components of Ca 2+ release-activated Ca 2+ (CRAC) channels that mediate store-operated Ca 2+ entry (SOCE) in immune cells....
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Isolation and identification of cytoskeleton-associated prolamine mRNA binding proteins from develo** rice seeds
The messenger RNA of the rice seed storage protein prolamine is targeted to the endoplasmic reticulum (ER) membranes surrounding prolamine protein...
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HPLC and Mass Spectrometry of Integral Membrane Proteins
HPLC separations of membrane proteins can be conveniently divided into two categories. Firstly, there are many methods available for isolation of... -
Develo** ontologies in decentralised settings
This paper addresses two research questions: “How should a well-engineered methodology facilitate the development of ontologies within communities of...
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Minimum Information about a Neuroscience Investigation (MINI) Electrophysiology
This module represents the formalized opinion of the authors and the CARMEN consortium, which identifies the minimum information required to report...
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The minimum information about a proteomics experiment (MIAPE)
Both the generation and the analysis of proteomics data are now widespread, and high-throughput approaches are commonplace. Protocols continue to...