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  1. Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells

    Hematopoietic stem cells (HSCs) are responsible for the generation and maintenance of pools of multipotent precursors that ultimately give rise to...
    Diego A. López, Anna E. Beaudin in Hematopoietic Stem Cells
    Protocol 2023
  2. Extraction, Isolation, and Characterization Techniques of Structural Proteins

    This chapter comprehensively explores the extraction methods, isolation techniques, and characterization approaches for structural proteins. It...
    Chapter 2024
  3. Human Hepatic Stellate Cells: Isolation and Characterization

    Liver fibrosis of different etiologies is characterized by activation of hepatic stellate cells (aHSCs) into collagen type I secreting...
    **ao Liu, David A. Brenner, Tatiana Kisseleva in Hepatic Stellate Cells
    Protocol 2023
  4. Primary Human Leukemia Stem Cell (LSC) Isolation and Characterization

    Leukemia stem cells (LSC) are thought to be the basis of leukemia progression since they are highly resistant to conventional chemotherapy. LSC...
    Neslihan Meriç, Fatih Kocabaş in Stem Cells and Lineage Commitment
    Protocol 2023
  5. Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation, Characterization, and Differentiation

    Adult mesenchymal stromal/stem cells (MSCs) are a standard component of de novo tissue generation to treat and study injury, disease, and...
    Takashi Taguchi, Mandi J. Lopez in Adipose-Derived Stem Cells
    Protocol 2024
  6. Isolation and Characterization of Extracellular Vesicles from Lymphocytes

    Extracellular vesicles (EVs) are nanosized particles secreted by all cells as a means of communication. When it comes to the immune system, most of...
    Lujain Al-Ghadir, Zhi Chen in Cell-Secreted Vesicles
    Protocol 2023
  7. Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy

    Extracellular vesicles (EVs) have the capacity for use in cartilage tissue engineering by stimulating tissue repair and microenvironmental...
    Alexander Otahal, Andrea De Luna, ... Stefan Nehrer in Cartilage Tissue Engineering
    Protocol 2023
  8. Isolation and molecular characterization of the Salmonella Typhimurium orphan phage Arash

    The current threat of multidrug resistant strains necessitates development of alternatives to antibiotics such as bacteriophages. This study...

    Mohammad Hashem Yousefi, Jeroen Wagemans, ... Saeid Hosseinzadeh in BMC Microbiology
    Article Open access 19 October 2023
  9. Platform for isolation and characterization of SARS-CoV-2 variants enables rapid characterization of Omicron in Australia

    Genetically distinct variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged since the start of the COVID-19 pandemic....

    Anupriya Aggarwal, Alberto Ospina Stella, ... Stuart G. Turville in Nature Microbiology
    Article Open access 30 May 2022
  10. Screening, Isolation and Characterization of Aerobic Magnetotactic Bacteria From Western Ghats Forest Soil

    Magnetotactic bacteria (MTB) are a unique ecophysiological group of iron-metabolizing bacteria that have immense potential biotechnological...

    Kruti Mistry, Anoop R. Markande, ... Kinnari Parekh in Indian Journal of Microbiology
    Article 27 May 2024
  11. Isolation and Characterization of Enterobacter , Klebsiella, and Clostridium

    The free-living nitrogen fixing bacteria (Enterobacter, Klebsiella, and Clostridium) play significant role in fixation of atmospheric nitrogen and...
    V. Mageshwaran, K. Pandiyan in Practical Handbook on Agricultural Microbiology
    Protocol 2022
  12. Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery

    Exosomes are small extracellular vesicles secreted by cells and are known to play a key role in intercellular communication. Several studies have...
    Lucas Trevisan França de Lima, Juliana Müller Bark, ... Chamindie Punyadeera in Extracellular Vesicles in Diagnosis and Therapy
    Protocol 2022
  13. Optimization of production conditions, isolation, purification, and characterization of tannase from filamentous fungi

    Tannase-producing filamentous fungi residing alongside tannin-rich ambient in the Northwest Himalayas were isolated at laboratory conditions and...

    Nisha Thakur, Amarjit K Nath, Amit Sharma in Folia Microbiologica
    Article 21 March 2024
  14. Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans

    Extracellular vesicles (EVs) are increasingly recognized as an important mechanism for cell–cell interactions. Their role in fungi is still poorly...

    Anja Černoša, Cene Gostinčar, ... Nina Gunde-Cimerman in Fungal Biology and Biotechnology
    Article Open access 01 November 2022
  15. Isolation and characterization of Rhizobium from non-leguminous potato plants: New frontiers in Rhizobium research

    Rhizobium is well-documented for its symbiotic relationship with legume plants, where it plays a crucial role in biological nitrogen-(N)-fixation...

    Tahir Naqqash, Kauser Abdullah Malik, ... Jan Dirk van Elsas in Biology and Fertility of Soils
    Article 17 February 2024
  16. Isolation, characterization, and application of bacteriophage cocktails for the biocontrol of Pseudomonas fluorescens group strains in whole and skimmed milk

    Pseudomonas fluorescens group strains can lead to spoilage of milk as well as loss of quality in dairy products through their heat-resistant enzymes....

    Emine Kubra Tayyarcan, Ismail Hakki Boyaci in Brazilian Journal of Microbiology
    Article 02 November 2023
  17. Isolation and characterization of 73 SNP markers in Schizopygopsis chengi baoxingensis

    Schizopygopsis chengi baoxingensis , a species belongs to Schizopygopsis group, is an endemic and rare fish in China. Due to the destruction of the...

    Mingjiang Song, Yeyu Chen, ... Jiansheng Lai in Conservation Genetics Resources
    Article 04 October 2023
  18. Isolation, Characterization and In Vitro Evaluation of Novel Lytic Phages Active Against Staphylococcus aureus and Escherichia coli of Bovine Mastitis Origin

    In this study, we report the isolation and characterization of novel lytic bacteriophages targeting Staphylococcus aureus and Escherichia coli ,...

    Article 14 June 2024
  19. Isolation and Characterization of Peritoneal Macrophages from Salamanders

    Salamanders have been used as research models for centuries. While they exhibit a wide range of biological features not seen in mammals, none has...
    Anthony Sallese, Georgios Tsissios, ... Katia Del Rio-Tsonis in Salamanders
    Protocol 2023
  20. Archaella Isolation

    Swimming archaea are propelled by a filamentous structure called the archaellum. The first step for the structural characterization of this filament...
    Shamphavi Sivabalasarma, João N. de Sousa Machado, ... Ken F. Jarrell in Bacterial and Archaeal Motility
    Protocol 2023
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