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Nucleoporin Elys attaches peripheral chromatin to the nuclear pores in interphase nuclei
Transport of macromolecules through the nuclear envelope (NE) is mediated by nuclear pore complexes (NPCs) consisting of nucleoporins (Nups)....
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Strong interactions between highly dynamic lamina-associated domains and the nuclear envelope stabilize the 3D architecture of Drosophila interphase chromatin
BackgroundInteractions among topologically associating domains (TADs), and between the nuclear envelope (NE) and lamina-associated domains (LADs) are...
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The chaperone DNAJB6 surveils FG-nucleoporins and is required for interphase nuclear pore complex biogenesis
Biogenesis of nuclear pore complexes (NPCs) includes the formation of the permeability barrier composed of phenylalanine-glycine-rich nucleoporins...
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What Actin and Myosin Do in the Nucleus: New Functions of the Well-Known Proteins
AbstractThe functions of actin and its motor proteins myosins in the cytoplasm have been the subject of research for more than 100 years, but the...
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The Physical Behavior of Interphase Chromosomes: Polymer Theory and Coarse-Grain Computer Simulations
Fluorescence in situ hybridization and chromosome conformation capture methods point to the same conclusion: that chromosomes appear to the external...
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Interphase epichromatin: last refuge for the 30-nm chromatin fiber?
“Interphase epichromatin” describes the surface of chromatin located adjacent to the interphase nuclear envelope. It was discovered in 2011 using a...
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Grease in the Nucleus: Insights into the Dynamic Life of Nuclear Membranes
Nucleus is at the center stage of cellular drama orchestrated in the life of a cell and the nucleoplasm is surrounded by a double membranous...
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RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure
DNA fluorescence in situ hybridization (FISH) has been a central technique in advancing our understanding of how chromatin is organized within the...
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Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
DNA transfection is an important technology in life sciences, wherein nuclear entry of DNA is necessary to express exogenous DNA. Non-viral vectors...
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Simulation of Different Three-Dimensional Models of Whole Interphase Nuclei Compared to Experiments - A Consistent Scale-Bridging Simulation Framework for Genome Organization
The three-dimensional architecture of chromosomes, their arrangement, and dynamics within cell nuclei are still subject of debate. Obviously, the...
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Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells
BackgroundThe cytoskeleton is a key component of the system responsible for transmitting mechanical cues from the cellular environment to the...
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Fission yeast condensin contributes to interphase chromatin organization and prevents transcription-coupled DNA damage
BackgroundStructural maintenance of chromosomes (SMC) complexes are central organizers of chromatin architecture throughout the cell cycle. The SMC...
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CTCF is essential for proper mitotic spindle structure and anaphase segregation
Mitosis is an essential process in which the duplicated genome is segregated equally into two daughter cells. CTCF has been reported to be present in...
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Direct imaging of intracellular RNA, DNA, and liquid–liquid phase separated membraneless organelles with Raman microspectroscopy
Methodologies for direct intracellular imaging of RNA and DNA are necessary for the advancement of bioimaging. Here we show direct label-free imaging...
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The Centrosome Cycle within the Cell Cycle
The synchronized distribution of centrosomal and genetic materials to the dividing daughter cells is critically important and depends on precisely...
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Lamin A as a Determinant of Mechanical Properties of the Cell Nucleus in Health and Disease
AbstractOne of the main factors associated with worse prognosis in oncology is metastasis, which is based on the ability of tumor cells to migrate...
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Multi-contact 3C reveals that the human genome during interphase is largely not entangled
During interphase, the eukaryotic genome is organized into chromosome territories that are spatially segregated into compartment domains. The extent...
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The probability of chromatin to be at the nuclear lamina has no systematic effect on its transcription level in fruit flies
BackgroundMultiple studies have demonstrated a negative correlation between gene expression and positioning of genes at the nuclear envelope (NE)...
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Assignment of the somatic A/B compartments to chromatin domains in giant transcriptionally active lampbrush chromosomes
BackgroundThe three-dimensional configuration of the eukaryotic genome is an emerging area of research. Chromosome conformation capture outlined...
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Measuring Cellular Ploidy In Situ by Light Microscopy
Determining cellular DNA content is valuable in the study of numerous biological processes, including organ development and injury repair. While FACS...