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Double-stranded RNA mediated knockdown of sucrase gene induced mortality and reduced offspring production in Aphis gossypii
The importance of gut sucrase in maintaining osmotic equilibrium and utilizing phloem contents as a carbon source has been widely investigated and...
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Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation
Expressing insecticidal double-stranded RNA (dsRNA)Double-stranded RNA (dsRNA) molecules in plant plastids is a novel approach for in planta...
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Characterization and potential mechanism of resistance to double-stranded RNA in willow leaf beetle, Plagiodera versicolora
RNAi-based pesticides have emerged gradually in recent decades and are believed to be the next generation of pesticides. Although resistance to RNA...
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Oligonucleotide insecticides and RNA-based insecticides: 16 years of experience in contact using of the next generation pest control agents
Recently, sustainable and ecological approach in plant protection has produced many innovative ideas, including insecticides based on nucleic acids....
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Molecular characterization of a novel double-stranded RNA virus infecting the entomopathogenic fungus Metarhizium brunneum
There are four dsRNAs segments present in the entomopathogenic fungus Metarhizium brunneum strain RCEF0766. The genomic segments dsRNA1 and dsRNA3...
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RNA interference (RNAi) mediated technique for combating plant diseases: Harnessing nanoparticles for effective delivery and enhanced efficacy
Crop production is severely affected by insects and plant pathogens causing one-third of the total produced food losses. Conventional agricultural...
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A first greenhouse application of bacteria-expressed and nanocarrier-delivered RNA pesticide for Myzus persicae control
There are two main limitations for sprayable RNA pesticide development: delivery efficiency and synthetic cost of double-stranded RNA (dsRNA). We...
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Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects
RNA interference (RNAi) has emerged as a widely used approach for reverse genetic analysis in eukaryotes. In insects, RNAi also has an application in...
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RNA Dialogues in Fungal–Plant Relationships
The role of extracellular RNAs in fungal–plant interaction has been neglected for long time due to its assumed instability. Recent advances in...
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RNA Interference Technology
RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation by neutralizing targeted mRNA...
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Guiding RNAi Design Through Characterization of Endogenous Small RNA Pathways
RNA interference (RNAi) is a common eukaryotic gene regulation process driven by small RNA effectors. Mechanisms that govern regulatory small...
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Different Generations of Genetically Modified Crops for Insect Resistance
Crops are exposed to a variety of insect pests throughout their lifetime. Insect pests cause significant damage to crop plants by feeding on their...
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Two insecticidal neurotoxins from parasitoid wasp Habrobracon hebetor venom and their potential use in biocontrol
The utilization of diverse entomopathogens like bacteria, viruses, or fungi holds promise in the biological control of insect pests. One approach to...
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Antisense RNA (asRNA) technology: the concept and applications in crop improvement and sustainable agriculture
Antisense RNA (asRNA) technology is a method used to silence genes and inhibit their expression. Gene function relies on expression, which follows...
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Diversity of transgenes in sustainable management of insect pests
Insecticidal transgenes, when incorporated and expressed in plants, confer resistance against insects by producing several products having...
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Development of a recombinant baculovirus with dual effects to mediate V-ATPase interference by RNA in the fall armyworm Spodoptera frugiperda
The fall armyworm (FAW) Spodoptera frugiperda has rapidly spread from the American continent to many other areas and caused serious damage to local...
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Insect Pest Management in Rice Through Genetic Engineering
Resistance breeding programs resulted in the successful development of rice cultivars expressing resistance to major insect pests of rice. However,...
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Natural Insecticidal Proteins and Their Potential in Future IPM
Increasing population and global food security is the foremost challenge for this century. Insect pests cause substantial damage to our crops by...
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Adoption of Molecular Tools for Combatting the Arthropod Pests
Monitoring of the harmful organisms and improvement of the beneficial organisms need a novel technology to work. The gene and genome editing is one...
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Negligible transcriptome and metabolome alterations in RNAi insecticidal maize against Monolepta hieroglyphica
Key messageRNAi-based genetically modified maize resistant to Monolepta hieroglyphica (Motschulsky) was demonstrated with negligible transcriptome...
