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  1. In Vitro Cytotoxicity Analysis: MTT/XTT, Trypan Blue Exclusion

    In vitro cytotoxicity analyses have played a major role in the current drug discovery processes. These analyses enable the measurement of target cell...
    Shalini Mani, Geeta Swargiary in Animal Cell Culture: Principles and Practice
    Chapter 2023
  2. Comprehensive multiscale analysis of lactate metabolic dynamics in vitro and in vivo using highly responsive biosensors

    As a key glycolytic metabolite, lactate has a central role in diverse physiological and pathological processes. However, comprehensive multiscale...

    Aoxue Wang, Yejun Zou, ... Yuzheng Zhao in Nature Protocols
    Article 02 February 2024
  3. Analysis of Golgi Protein Acetylation Using In Vitro Assays and Parallel Reaction Monitoring Mass Spectrometry

    Acetylation is one of the most abundant post-translational protein modifications that regulates all cellular compartments ranging from chromatin to...
    Dea Slade, Markus Hartl in Golgi
    Protocol 2023
  4. In vitro and In vivo analysis of probiotic yeasts for dietary supplementation in Indian major carps

    Five probiotic yeast strains viz. Yarrowia lipolytica VIT-MN01, Kluyveromyces lactis VIT-MN02, Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligera ...

    Mangala Lakshmi Ragavan, Nilanjana Das in Biologia
    Article 05 June 2023
  5. In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis

    So far, in Bacillus subtilis, only four trans-encoded and 11 cis-encoded sRNAs and their targets have been investigated in detail, the majority of...
    Inam Ul Haq, Peter Müller, Sabine Brantl in Bacterial Regulatory RNA
    Protocol 2024
  6. New Insights for the Production of Medicinal Plant Materials: Ex Vitro and in Vitro Propagation

    Medicinal plants are a vital input of raw materials for the manufacture of pharmaceuticals to treat various ailments of humans and animals. The...
    H. I. G. K. Anuruddi, Niluka Nakandalage, D. L. C. Kumari Fonseka in Biosynthesis of Bioactive Compounds in Medicinal and Aromatic Plants
    Chapter 2023
  7. Antifibrotic effect of silymarin on arecoline-induced fibrosis in primary human buccal fibroblasts: an in silico and in vitro analysis

    Background

    This study aimed to assess silymarin’s anticancer and antifibrotic potential through in silico analysis and investigate its impact on in...

    Divyambika Catakapatri Venugopal, Paramesh Viswanathan, ... Vijayalakshmi Ramshankar in Molecular Biology Reports
    Article 15 February 2024
  8. Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis

    By affecting the ovarian pool of follicles and their enclosed oocytes, heat stress has an impact on dairy cow fertility. This study aimed to...

    Hayder Radhi Hussein Mzedawee, Rasoul Kowsar, ... Mehdi Hajian in Amino Acids
    Article Open access 27 January 2024
  9. Insights Into the Morphological Analysis, Phytochemical Profile and Antimicrobial Activities of the Multi-Walled Carbon Nanotubes-Induced In Vitro Sugarcane

    The present research work reports the impact of multi-walled carbon nanotubes (MWCNTs) on sugarcane morphological characteristics, the production of...

    Aneela Iqbal, Muhammad Usman, ... Raham Sher Khan in Sugar Tech
    Article 17 May 2024
  10. In Vitro Testing of Heart Valve Substitutes

    This chapter summarizes in vitro test methods for qualifying the design and manufacture of a heart valve substitute. The tests discussed herein are...
    Timothy A. Kelley, Sal Marquez, ... Matthew C. Ziebol in Heart Valves
    Chapter 2023
  11. Analysis of Neutrophil Morphology and Function Under Genetic Perturbation of Transcription Factors In Vitro

    Hoxb8 cells are immortalized myeloid progenitors that maintain their multipotent potential and can be differentiated into neutrophils. Genetic...
    Julia Salafranca, Zhichao Ai, ... Erinke van Grinsven in Transcription Factor Regulatory Networks
    Protocol 2023
  12. In vitro induction of tetraploids and their phenotypic and transcriptome analysis in Glehnia littoralis

    Background

    Glehnia littoralis is a medicinal and edible plant species having commercial value and has several hundred years of cultivation history....

    **n Zhang, Ziyu Zheng, ... Fuhua Bian in BMC Plant Biology
    Article Open access 22 May 2024
  13. Improved in vitro regeneration, genetic fidelity analysis, antioxidant potential, and hairy root induction of Justicia gendarussa Burm. f

    Justicia gendarussa Burm. f. is an indispensable medicinal plant owing to its abundance of phytoconstituents and medicinal uses. In the study, we...

    Muthiah Joe Virgin Largia, Subramani Pandian, ... Manikandan Ramesh in Plant Biotechnology Reports
    Article 05 July 2022
  14. ChIP-Seq Protocol for In Vitro Cell Differentiation Systems

    In vitro differentiation systems provide a flexible platform for understanding complex developmental processes. Here, we provide a comprehensive...
    Jacqueline Copeland, Fjodor Merkuri, Marcos Simoes-Costa in DNA-Protein Interactions
    Protocol 2023
  15. Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells

    Gene regulation in the nucleus requires precise control of the molecular processes that dictate how, when, and which genes are transcribed. The...
    Dan Huang, Andrea D. Edwards, ... W. Lee Kraus in Poly(ADP-Ribose) Polymerase
    Protocol 2023
  16. Large-Scale Production of Specialized Metabolites In Vitro Cultures

    For centuries plants have been intensively utilized as reliable sources of food, flavoring, and pharmaceutical ingredients. However, plant natural...
    Andrey S. Marchev, Iva D. Stoykova, Milen I. Georgiev in Plant Cell Culture Protocols
    Protocol 2024
  17. In Vitro Titan Cell Generation in Cryptococcus neoformans and Automated Cell Size Measurements

    A special feature of the human fungal pathogen Cryptococcus neoformans is its morphological changes triggered by the interaction with the host....
    Benjamin Hommel, Aude Sturny-Leclère, Alexandre Alanio in Cryptococcus neoformans
    Protocol 2024
  18. In Vitro Culture of Arabidopsis Embryos

    Arabidopsis thaliana is currently the most important model organism for basic molecular plant research. It is also a favourable model for...
    Michael Sauer, Jiří Friml in Somatic Embryogenesis
    Chapter
  19. Analysis of clonogenic growth in vitro

    The clonogenic assay measures the capacity of single cells to form colonies in vitro. It is widely used to identify and quantify self-renewing...

    Nikko Brix, Daniel Samaga, ... Kirsten Lauber in Nature Protocols
    Article 25 October 2021
  20. In Vitro Gamma Mutagenesis Techniques in Rice (Oryza sativa L. var. Lazarroz FL)

    Gamma radiation (60Co)-induced mutagenesis offers an alternative to develop rice lines by accelerating the spontaneous mutation process and...
    Jason Pérez, Alejandro Hernández-Soto, ... Andrés Gatica-Arias in Plant Functional Genomics
    Protocol 2024
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