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In Vitro Cytotoxicity Analysis: MTT/XTT, Trypan Blue Exclusion
In vitro cytotoxicity analyses have played a major role in the current drug discovery processes. These analyses enable the measurement of target cell... -
Comprehensive multiscale analysis of lactate metabolic dynamics in vitro and in vivo using highly responsive biosensors
As a key glycolytic metabolite, lactate has a central role in diverse physiological and pathological processes. However, comprehensive multiscale...
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Analysis of Golgi Protein Acetylation Using In Vitro Assays and Parallel Reaction Monitoring Mass Spectrometry
Acetylation is one of the most abundant post-translational protein modifications that regulates all cellular compartments ranging from chromatin to... -
In vitro and In vivo analysis of probiotic yeasts for dietary supplementation in Indian major carps
Five probiotic yeast strains viz. Yarrowia lipolytica VIT-MN01, Kluyveromyces lactis VIT-MN02, Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligera ...
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In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis
So far, in Bacillus subtilis, only four trans-encoded and 11 cis-encoded sRNAs and their targets have been investigated in detail, the majority of... -
New Insights for the Production of Medicinal Plant Materials: Ex Vitro and in Vitro Propagation
Medicinal plants are a vital input of raw materials for the manufacture of pharmaceuticals to treat various ailments of humans and animals. The... -
Antifibrotic effect of silymarin on arecoline-induced fibrosis in primary human buccal fibroblasts: an in silico and in vitro analysis
BackgroundThis study aimed to assess silymarin’s anticancer and antifibrotic potential through in silico analysis and investigate its impact on in...
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Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis
By affecting the ovarian pool of follicles and their enclosed oocytes, heat stress has an impact on dairy cow fertility. This study aimed to...
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Insights Into the Morphological Analysis, Phytochemical Profile and Antimicrobial Activities of the Multi-Walled Carbon Nanotubes-Induced In Vitro Sugarcane
The present research work reports the impact of multi-walled carbon nanotubes (MWCNTs) on sugarcane morphological characteristics, the production of...
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In Vitro Testing of Heart Valve Substitutes
This chapter summarizes in vitro test methods for qualifying the design and manufacture of a heart valve substitute. The tests discussed herein are... -
Analysis of Neutrophil Morphology and Function Under Genetic Perturbation of Transcription Factors In Vitro
Hoxb8 cells are immortalized myeloid progenitors that maintain their multipotent potential and can be differentiated into neutrophils. Genetic... -
In vitro induction of tetraploids and their phenotypic and transcriptome analysis in Glehnia littoralis
BackgroundGlehnia littoralis is a medicinal and edible plant species having commercial value and has several hundred years of cultivation history....
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Improved in vitro regeneration, genetic fidelity analysis, antioxidant potential, and hairy root induction of Justicia gendarussa Burm. f
Justicia gendarussa Burm. f. is an indispensable medicinal plant owing to its abundance of phytoconstituents and medicinal uses. In the study, we...
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ChIP-Seq Protocol for In Vitro Cell Differentiation Systems
In vitro differentiation systems provide a flexible platform for understanding complex developmental processes. Here, we provide a comprehensive... -
Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells
Gene regulation in the nucleus requires precise control of the molecular processes that dictate how, when, and which genes are transcribed. The... -
Large-Scale Production of Specialized Metabolites In Vitro Cultures
For centuries plants have been intensively utilized as reliable sources of food, flavoring, and pharmaceutical ingredients. However, plant natural... -
In Vitro Titan Cell Generation in Cryptococcus neoformans and Automated Cell Size Measurements
A special feature of the human fungal pathogen Cryptococcus neoformans is its morphological changes triggered by the interaction with the host.... -
In Vitro Culture of Arabidopsis Embryos
Arabidopsis thaliana is currently the most important model organism for basic molecular plant research. It is also a favourable model for... -
Analysis of clonogenic growth in vitro
The clonogenic assay measures the capacity of single cells to form colonies in vitro. It is widely used to identify and quantify self-renewing...
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In Vitro Gamma Mutagenesis Techniques in Rice (Oryza sativa L. var. Lazarroz FL)
Gamma radiation (60Co)-induced mutagenesis offers an alternative to develop rice lines by accelerating the spontaneous mutation process and...