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Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos
A commonly employed technique in molecular biology to evaluate the temporal and spatial expression of a certain gene is in situ hybridization. This...
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A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
BackgroundIn the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is...
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Colorimetric Whole-Mount In Situ Hybridization in Planarians
Whole-mount in situ hybridization (WISH) is an extremely useful technique for visualizing specific mRNA targets and solving many biological...
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Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea
Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust...
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Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvae
Whole-mount in situ hybridization (WISH) is widely used to visualize transcribed gene sequences (mRNA) in develo** embryos, larvae, and other...
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Whole-mount RNA in situ hybridization technique in Torenia ovules
The expression pattern of an interested gene at a cellular level provides strong evidence for its functions. RNA in situ hybridization has been...
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Single-Molecule Fluorescent In Situ Hybridization (smFISH) on Whole-Mount Planarians
Since the establishment of planarian species as laboratory models, investigation of molecular pathways has relied heavily on visualization of...
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Whole-Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells
One of the most important events in early vertebrate development is the formation and positioning of the endoderm, the embryonic progenitor cell...
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Analysis of Gene Expression Patterns and RNA Localization by Fluorescence in Situ Hybridization in Whole Mount Drosophila Testes
Researchers have used RNA in situ hybridization to detect the presence of RNA in cells and tissues for approximately 50 years. The recent development...
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Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue
In situ hybridization is a standard procedure for visualizing mRNA transcripts in tissues. The recent adoption of fluorescent probes and new signal...
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Whole mount multiplexed visualization of DNA, mRNA, and protein in plant-parasitic nematodes
BackgroundPlant-parasitic nematodes compromise the agriculture of a wide variety of the most common crops worldwide. Obtaining information on the...
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3D exploration of gene expression in chicken embryos through combined RNA fluorescence in situ hybridization, immunofluorescence, and clearing
BackgroundFine characterization of gene expression patterns is crucial to understand many aspects of embryonic development. The chicken embryo is a...
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Gene Expression Patterns in the Ctenophore Pleurobrachia bachei: In Situ Hybridization
In situ hybridization is a powerful and precise tool for revealing cell- and tissue-specific gene expression and a critical approach to validating...
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Formamide-Free Genomic In Situ Hybridization (ff-GISH)
Fluorescence in situ hybridization allows for the map** of various sequence types in the genomes and is thus widely used in structural, functional,...
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Whole-Mount RNA, Single-Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos
Fluorescence in situ hybridization (FISH) provides a valuable tool for studying the spatial localization of and expression level of genes and cell...
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Whole-mount smFISH allows combining RNA and protein quantification at cellular and subcellular resolution
Multicellular organisms result from complex developmental processes largely orchestrated through the quantitative spatiotemporal regulation of gene...
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Novel whole-mount FISH analysis for intact root of Arabidopsis thaliana with spatial reference to 3D visualization
Whole-mount fluorescent in situ hybridization (WM-FISH) is an effective tool to observe chromosome behavior in tissues or organs. However, it is...
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Combined whole-mount fluorescence in situ hybridization and antibody staining in zebrafish embryos and larvae
RNA fluorescence in situ hybridization (FISH) and antibody staining/immunofluorescence (IF) are widely used to detect distributions of mRNAs and...
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Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA in Situ Hybridization and Immunocytochemistry
High-content fluorescence microscopy combines the efficiency of high-throughput techniques with the ability to extract quantitative information from...
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In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis
Sponges (Porifera) are a large phylum that includes an enormous number of species. They are classified into four classes. Among these four classes,...