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Fluorescence In Situ Hybridization (FISH) Methods and Protocols
This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically...
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Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos
A commonly employed technique in molecular biology to evaluate the temporal and spatial expression of a certain gene is in situ hybridization. This...
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Unequivocal Identification Through Fluorescence In Situ Hybridization (FISH) Technique
Protists represent the largest portion of the diversity of eukaryotes in the globe and have been recognized for performing indispensable activities...
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Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue
In situ hybridization is a standard procedure for visualizing mRNA transcripts in tissues. The recent adoption of fluorescent probes and new signal...
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Formamide-Free Genomic In Situ Hybridization (ff-GISH)
Fluorescence in situ hybridization allows for the map** of various sequence types in the genomes and is thus widely used in structural, functional,...
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Gene Expression Patterns in the Ctenophore Pleurobrachia bachei: In Situ Hybridization
In situ hybridization is a powerful and precise tool for revealing cell- and tissue-specific gene expression and a critical approach to validating...
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Detection of dsRNA with Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or...
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Thyramide Signal Amplification: New Opportunities for DNA in Situ Hybridization
Abstract —DNA in situ hybridization (ISH) is a valuable technique in molecular cytogenetics for precisely localizing specific DNA sequences on...
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Visualization of Individual RNA Molecules by Proximity Ligation-Based Chromogenic In Situ Hybridization Assay
RNA in situ hybridization reveals the abundance and location of gene expression in cells or tissues, providing a technical basis for the clinical...
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Colorimetric Whole-Mount In Situ Hybridization in Planarians
Whole-mount in situ hybridization (WISH) is an extremely useful technique for visualizing specific mRNA targets and solving many biological...
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Detection of MicroRNAs in Brain Slices Using In Situ Hybridization
MicroRNAs are key posttranscriptional regulators of protein levels in cells. The brain is particularly enriched in microRNAs, and important roles...
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Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea
Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust...
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Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvae
Whole-mount in situ hybridization (WISH) is widely used to visualize transcribed gene sequences (mRNA) in develo** embryos, larvae, and other...
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Single-Molecule Fluorescent In Situ Hybridization (smFISH) on Whole-Mount Planarians
Since the establishment of planarian species as laboratory models, investigation of molecular pathways has relied heavily on visualization of...
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Fluorescence In Situ Hybridization as a Tool for Studying the Specification and Differentiation of Cell Types in Nematostella vectensis
The sea anemone Nematostella vectensis is a genetically tractable cnidarian species that has become a model organism for studying the evolution of...
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Multiplexed Immunofluorescence and Single-Molecule RNA Fluorescence In Situ Hybridization in Mouse Skeletal Myofibers
RNA fluorescence in situ hybridization (FISH) is a powerful method to determine the abundance and localization of mRNA molecules in cells. While...
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Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians
The capability to simultaneously apply different molecular tools to visualize a wide variety of changes in genetic expression and tissue composition...
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Whole-mount RNA in situ hybridization technique in Torenia ovules
The expression pattern of an interested gene at a cellular level provides strong evidence for its functions. RNA in situ hybridization has been...
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A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
BackgroundIn the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is...
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Fluorescence In Situ Hybridization of DNA Probes on Mitotic Chromosomes of the Mexican Axolotl
Fluorescence in situ hybridization (FISH) is used extensively for visual localization of specific DNA fragments (and RNA fragments) in broad...