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L-form conversion in Gram-positive bacteria enables escape from phage infection
At the end of a lytic bacteriophage replication cycle in Gram-positive bacteria, peptidoglycan-degrading endolysins that cause explosive cell lysis...
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Transcriptomic changes in single yeast cells under various stress conditions
BackgroundThe stress response of Saccharomyces cerevisiae has been extensively studied in the past decade. However, with the advent of recent...
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Improved sensitivity in cell surface protein detection by combining chemical labeling with mechanical lysis in a colorectal cancer cell model
ObjectiveTo develop a new technique for improved cell surface protein detection and analysis by combining chemical labeling with mild cell lysis...
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Cell Cycle–Specific Protein Phosphatase 1 (PP1) Substrates Identification Using Genetically Modified Cell Lines
The identification of protein phosphatase 1 (PP1) holoenzyme substrates has proven to be a challenging task. PP1 can form different holoenzyme...
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In Vivo and In Vitro Assay to Address Dendritic Cell Antigen Cross-Presenting Capacity
Antigen cross-presentation by dendritic cells is an important pathway to prime CD8+ T cells in infections, cancer, and other immune-mediated...
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Live Cell Synthetic Histone Acetylation by Chemical Catalyst
Posttranslational modifications (PTMs) of histonesPosttranslational modifications (PTMs) of histones, such as lysine acetylation and...
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Assessing the Activity of Transcription Factor FoxO1
The transcription factor FoxO1 (forkhead box O1) regulates genes that are involved in development, metabolism, cellular innovation, longevity, and...
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MS2-MBP-Based Affinity Purification of Nucleus- or Cytoplasm-Localized lncRNA–Protein Complexes Formed In Vivo
With recent emergence of huge number of long noncoding RNAs (lncRNAs), purification of lncRNA–protein (lncRNP) complexes is fundamental to understand...
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Conventional and Basic Genomic Techniques
In this chapter, certain very basic and primary technologies pertaining to genomics have been recapitulated. The topics covered include basic...
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Isolation of Cytosolic Ribosomes Associated with Plant Mitochondria and Chloroplasts
Excluding the few dozen proteins encoded by the chloroplast and mitochondrial genomes, the majority of plant cell proteins are synthesized by...
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Bioprospective studies of pigmented ink from Sepioteuthis lessoniana and its molecular identification using CO1 gene
BackgroundThe present-day world is teeming with numerous diseases due to the changing environment. The enormous growth of population has overburdened...
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SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling
Vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) signaling pathways are tightly regulated multistep chain reactions that involve a...
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Analysis of mRNA Translation by Polysome Profiling
mRNA translation is a key step in gene expression that allows the cell to qualitatively and quantitatively modulate the cell’s proteome according to...
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Isolation of Glycosomes from Trypanosoma brucei
Glycosomes, belonging to the sub-class of peroxisomes, are single-membrane-bound organelles of trypanosomatid parasites. Glycosomes compartmentalize...
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The trend of allogeneic tendon decellularization: literature review
Tendon injuries repair is a significant burden for orthopaedic surgeons. Finding a proper graft material to repair tendon is one of the main...
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Isolating Mitochondria, Mitoplasts, and mtDNA from Cultured Mammalian Cells
Mitochondria are double membrane-bound eukaryotic organelles with roles in a range of cellular activities including energy conversion, apoptosis,...
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Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+
The bacterium Escherichia coli is still considered the first option as a microbial cell factory for recombinant protein production, and affinity...
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Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2): A Cation Channel Activated by ADP-Ribose and Involved in Cell Death
Transient receptor potential melastatin-2 (TRPM2) is an emerging chemotherapeutic target due to its involvement in poly(ADP-ribose) metabolism and...
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Isolation of Polystyrene Bead-Induced Phagosomes for Western Blotting
The engulfment of “self” and “non-self” particles by immune and non-immune cells is crucial for maintaining homeostasis and combatting infection....
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Isolation and Purification of Chromaffin Granules from Adrenal Glands and Cultured Neuroendocrine Cells
Chromaffin granules isolated from adrenal glands constitute a powerful experimental tool to the study of secretory vesicle components and their...