Search
Search Results
-
Pcal_0976, a pullulanase homologue from Pyrobaculum calidifontis, displays a glycoside hydrolase activity but no pullulanase activity
In this study, we have cloned and characterized a novel protein, Pcal_0976, annotated as pullulanase in the genome sequence of hyperthermophilic...
-
Impairing hydrolase transport machinery prevents human melanoma metastasis
Metastases are the major cause of cancer-related death, yet, molecular weaknesses that could be exploited to prevent tumor cells spreading are poorly...
-
Improvement of the Catalytic Activity of Thermoacidophilic Pullulan Hydrolase Type III by Error-Prone PCR Technology
AbstractThermoacidophilic pullulan hydrolase type III from Thermococcus kodakarensis (TK-PUL) completely hydrolyzes starch under liquefaction...
-
Improving ethanol tolerance of ethyl carbamate hydrolase by diphasic high pressure molecular dynamic simulations
Ethyl carbamate (EC) is mainly found in fermented foods and fermented alcoholic beverages, which could cause carcinogenic potential to humans....
-
Recent advances in enzymatic properties, preparation methods, and functions of glycoside hydrolase from Bifidobacterium: a review
Bifidobacterium is a major probiotic of intestinal gut flora and exerts many physiological activities, and it is widely applied in the fields of food...
-
Radiometric Assay of ABHD2 Activity
The α,β-hydrolase fold-containing protein 2 (ABHD2) is a serine hydrolase, responsible for the cleavage of endogenous 2-arachidonoylglycerol (2-AG)....
-
Capturing acyl–enzyme intermediates with genetically encoded 2,3-diaminopropionic acid for hydrolase substrate identification
Catalytic mechanism-based, light-activated traps have recently been developed to identify the substrates of cysteine or serine hydrolases. These...
-
Radiometric Assay of FAAH Activity
Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that...
-
-
Clostridioides difficile bile salt hydrolase activity has substrate specificity and affects biofilm formation
The Clostridioides difficile pathogen is responsible for nosocomial infections. Germination is an essential step for the establishment of C. difficile ...
-
The Expression Levels of Genes Responsible for the Enzymatic Activity of Bile Salt Hydrolase (BSH) and the Relationship of Cholesterol Assimilation in L. plantarum and L. paracasei
The bile salt hydrolase (BSH) activity is responsible for the cholesterol-lowering effect of the probiotic strains. The present study aimed to...
-
The Cholesterol-5,6-Epoxide Hydrolase: A Metabolic Checkpoint in Several Diseases
Cholesterol-5,6-epoxides (5,6-ECs) are oxysterols (OS) that have been linked to several pathologies including cancers and neurodegenerative diseases....
-
A new and promiscuous α/β hydrolase from Acinetobacter tandoii DSM 14970 T inactivates the mycotoxin ochratoxin A
AbstractThe presence of ochratoxin A (OTA) in food and feed represents a serious concern since it raises severe health implications. Bacterial...
-
Fluorimetric Assay of FAAH Activity
Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the degradation of anandamide (N-arachidonoylethanolamine, AEA) to arachidonic acid...
-
Identification of a novel glycoside hydrolase family 8 xylanase from Deinococcus geothermalis and its application at low temperatures
Xylanase is the most important hydrolase in the xylan hydrolase system, the main function of which is β-1,4-endo-xylanase, which randomly cleaves...
-
Identification of novel extracellular putative chitinase and hydrolase from Geomyces sp. B10I with the biodegradation activity towards polyesters
Cold-adapted filamentous fungal strain Geomyces sp. B10I has been reported to decompose polyesters such as poly(e-caprolactone) (PCL), poly(butylene...
-
Essential amino acid residues and catalytic mechanism of trans-epoxysuccinate hydrolase for production of meso-tartaric acid
ObjectivesThis study aimed to discuss the essential amino acid residues and catalytic mechanism of trans -epoxysuccinate hydrolase from Pseudomonas...
-
Glycoside hydrolase processing of the Pel polysaccharide alters biofilm biomechanics and Pseudomonas aeruginosa virulence
Pel exopolysaccharide biosynthetic loci are phylogenetically widespread biofilm matrix determinants in bacteria. In Pseudomonas aeruginosa , Pel is...
-
Potential of Y. lipolytica epoxide hydrolase for efficient production of enantiopure (R)-1,2-octanediol
The recombinant Yleh from a tropical marine yeast Yarrowia lipolytica NCIM 3589 exhibited a high epoxide hydrolase activity of 9.34 ± 1.80 µmol min -1 mg...
-
The global distribution of the macrolide esterase EstX from the alpha/beta hydrolase superfamily
Macrolide antibiotics, pivotal in clinical therapeutics, are confronting resistance challenges mediated by enzymes like macrolide esterases, which...
