Search
Search Results
-
Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment
Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis are usually heterozygous...
-
Characterization of homozygous Foxn1 mutations induced in rat embryos by different delivery forms of Cas9 nuclease
BackgroundThe Cas9 nuclease is delivered in the form of either Cas9 protein or mRNA along with CRISPR guide RNA (gRNA: dual-crRNA:tracrRNA or...
-
A guide to barley mutants
BackgroundMutants have had a fundamental impact upon scientific and applied genetics. They have paved the way for the molecular and genomic era, and...
-
New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i
BackgroundFor ten years, CRISPR/cas9 system has become a very useful tool for obtaining site-specific mutations on targeted genes in many plant...
-
T-DNA insertion mutants of Arabidopsis DA1 orthologous genes displayed altered plant height and yield-related traits in rice (O. sativa L.)
BackgroundThe Arabidopsis DA1 gene is a key player in the regulation of organ and seed development. To extend our understanding of its functional...
-
Strategies for Screening Induced Mutants for Stress Tolerance
Crop plants are exposed to a multitude of biotic and abiotic factors, which necessitates improving tolerance/resistance in plant germplasm using...
-
C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases
Loss-of-function mutants are fundamental resources for gene function studies. However, it is difficult to generate viable and heritable knockout...
-
Zebrafish nampt-a mutants are viable despite perturbed primitive hematopoiesis
BackgroundNicotinamide phosphoribosyltransferase (Nampt) is required for recycling NAD + in numerous cellular contexts. Morpholino-based knockdown of...
-
Construction of homozygous diploid potato through maternal haploid induction
Reinventing the tetraploid potato into a seed-propagated, diploid, hybrid potato would significantly accelerate potato breeding. In this regard, the...
-
Role of Silicon in Mediating Salt Stress Responses in Arabidopsis Methylation Mutants
PurposeThe growing prevalence of soil salinity presents a significant threat to agriculture production on a global scale. Previous studies on salt...
-
HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells
Recent developments in auxin-inducible degron (AID) technology have increased its popularity for chemogenetic control of proteolysis. However,...
-
Altered Signaling and Desensitization Responses in PTH1R Mutants Associated with Eiken Syndrome
The parathyroid hormone receptor type 1 (PTH1R) is a G protein-coupled receptor that plays key roles in regulating calcium homeostasis and skeletal...
-
High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9
Key message4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods....
-
Comparative study of PRPH2 D2 loop mutants reveals divergent disease mechanism in rods and cones
Mutations in the photoreceptor-specific tetraspanin gene peripherin-2 ( PRPH2 ) lead to widely varying forms of retinal degeneration ranging from...
-
Generation of stable homozygous transformants of diploid yeasts such as Xanthophyllomyces dendrorhous
AbstractThe nonconventional yeast Xanthophyllomyces dendrorhous is an established platform for genetic pathway modification. A genetic tool box is...
-
The Characterization of Arabidopsis Photorespiration D-glycerate 3-Kinase Mutants Generated by CRISPR/Cas9 and Identification of Its Interacting Proteins
D-Glycerate 3-kinase (GLYK, EC2.7.1.31) catalyzes the phosphorylation of glycerate to form 3-phosphoglycerate. However, knowledge about GLYK...
-
Integrin alpha 6 homozygous splice-site mutation causes a new form of junctional epidermolysis bullosa in Charolais cattle
BackgroundInherited epidermolysis bullosa (EB) is a group of painful and life-threatening genetic disorders that are characterized by mechanically...
-
Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco
BackgroundPhotosynthetic pigments in higher plants, including chlorophyll (Chl) and carotenoids, are crucial for photosynthesis and photoprotection....
-
Characterization of thylakoid division using chloroplast dividing mutants in Arabidopsis
Chloroplasts are double membrane bound organelles that are found in plants and algae. Their division requires a number of proteins to assemble into...
-
CRISPR/Cas9-based generation of mlo mutants for allelic complementation experiments to elucidate MLO function in barley
Barley ( Hordeum vulgare ) mildew locus o ( mlo ) mutants exhibit strong resistance to penetration by the powdery mildew fungus Blumeria graminis f. sp. hordei...
