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Diversity of H2A Histones and Their Effect on Nucleosome Structural Properties
AbstractHistone proteins that play an important role in the chromatin dynamics and regulation of gene activity are a key epigenetic factor. They are...
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Self-assembling viral histones are evolutionary intermediates between archaeal and eukaryotic nucleosomes
Nucleosomes are DNA–protein complexes composed of histone proteins that form the basis of eukaryotic chromatin. The nucleosome was a key innovation...
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Histones direct site-specific CRISPR spacer acquisition in model archaeon
CRISPR–Cas systems provide heritable immunity against viruses and other mobile genetic elements by incorporating fragments of invader DNA into the...
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Histones with an unconventional DNA-binding mode in vitro are major chromatin constituents in the bacterium Bdellovibrio bacteriovorus
Histone proteins bind DNA and organize the genomes of eukaryotes and most archaea, whereas bacteria rely on different nucleoid-associated proteins....
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Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA
Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions....
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Viral histones: pickpocket’s prize or primordial progenitor?
The common histones H2A, H2B, H3, and H4 are the characteristic components of eukaryotic nucleosomes, which function to wrap DNA and compact the...
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Trauma-associated extracellular histones mediate inflammation via a MYD88-IRAK1-ERK signaling axis and induce lytic cell death in human adipocytes
Despite advances in the treatment and care of severe physical injuries, trauma remains one of the main reasons for disability-adjusted life years...
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Ellman’s reagent prevents dephosphorylation of histones during isolation of mitotic chromosomes
Histones H1 and H3 are highly phosphorylated in mitotic HeLa cells but are rapidly dephosphorylated by endogenous protein phosphatases during the...
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Extra-nuclear histones: origin, significance and perspectives
Histones are classically known to organize the eukaryotic DNA into chromatin. They are one of the key players in regulating transcriptionally...
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Structural comparisons reveal diverse binding modes between nucleosome assembly proteins and histones
Nucleosome assembly proteins (NAPs) are histone chaperones that play a central role in facilitating chromatin assembly/disassembly which is of...
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Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles
Expression and purificationHistonespurification of individual histone proteins and amplification and purification of DNA are the initial steps toward...
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Native and tagged CENP-A histones are functionally inequivalent
BackgroundOver the past several decades, the use of biochemical and fluorescent tags has elucidated mechanistic and cytological processes that would...
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Quantitative Analysis of Posttranslational Modifications of Plant Histones
Resha** of the chromatin landscape under oxidative stress is of paramount importance for mounting an effective stress response. Unbiased systemic...
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Modeling of covalent modifications of histones to estimate the binding affinity
Covalent histone modifications such as methylation, acetylation, phosphorylation, and other epigenetic modifications of the chromatin play an...
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Cataloging Posttranslational Modifications in Plant Histones
Eukaryotic DNA exist in the nuclei in the form of a complex with proteins called chromatin. Access to the information encoded in the DNA requires the...
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The Role of Linker Histones in Carcinogenesis
Abstract —Linker histones are DNA-binding architectural chromatin proteins involved in the formation of supranucleosomal levels of chromatin...
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Identifying Specific Protein Interactors of Nucleosomes Carrying Methylated Histones Using Quantitative Mass Spectrometry
Chemical modification of histone proteins by methylation plays a central role in chromatin regulation by recruiting epigenetic “readers” via...
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Histones on fire: the effect of Dun1 and Mrc1 on origin firing and replication of hyper-acetylated genomes
As cells replicate their DNA, there is a need to synthesize new histones with which to wrap it. Newly synthesized H3 histones that are incorporated...
