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Microbial Production of Heparosan
Heparosan is a linear polysaccharide comprised of repetitive disaccharide units of α-1,4-N-acetylglucosamine and β-1,4-glucuronic acid. It is...
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Methods for the Production of Recombinant Heparosan , a Critical Heparin Precursor, from Nonpathogenic E. coli Strains
Heparin is an essential anticoagulant drug discovered over a century ago. Heparin is the second most highly used natural drug and remains a mainstay...
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High density fermentation of probiotic E. coli Nissle 1917 towards heparosan production, characterization, and modification
AbstractHeparosan is a naturally occurring non-sulfated glycosaminoglycan. Heparosan serves as the substrate for chemoenzymatic synthesis of...
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A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides
Carbohydrate-Active enZYme (CAZY) GH89 family enzymes catalyze the cleavage of terminal α- N -acetylglucosamine from glycans and glycoconjugates....
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Chemical Approaches to Prepare Modified Heparin and Heparosan Polymers for Biological Studies
Heparin is a potent clinically used anticoagulant. It is a heterogeneous mixture of polymers that contain a variety of sulfation patterns. Heparin...
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One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan
Heparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5...
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The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases
ObjectivesHeparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used...
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Molecular weight determination of heparosan- and chondroitin-like capsular polysaccharides: figuring out differences between wild -type and engineered Escherichia coli strains
Heparin and chondroitin sulfate are used as anti-thrombic and anti-osteoarthritis drugs, respectively, but their pharmacological actions depend on...
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Chemical O-sulfation of N-sulfoheparosan: a route to rare N-sulfo-3-O-sulfoglucosamine and 2-O-sulfoglucuronic acid
Heparosan, the capsular polysaccharide of E. coli K5 is currently used as the starting material in the chemoenzymatic synthesis of heparan sulfate...
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Inhibition of bacterial swimming by heparin binding of flagellin FliC from Escherichia coli strain Nissle 1917
Escherichia coli Nissle1917 (EcN) is a non-pathogenic probiotic strain widely used to maintain gut health, treat gastrointestinal disorders, and...
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Preparation of Isotope-Enriched Heparan Sulfate Precursors for Structural Biology Studies
Heparan sulfate (HS) plays numerous important roles in biological systems through their interactions with a wide array of proteins. Structural...
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Preparation and Characterization of Heparan Sulfate-Derived Oligosaccharides to Investigate Protein–GAG Interaction and HS Biosynthesis Enzyme Activity
Heparan sulfate chains are complex and structurally diverse polysaccharides that interact with a large number of proteins, thereby regulating a vast...
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Chemoenzymatic quantification for monitoring unpurified polysaccharide in rich medium
The heparosan polysaccharide serves as the starting carbon backbone for the chemoenzymatic synthesis of heparin, a widely used clinical anticoagulant...
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Microbial Functional Foods and Nutraceuticals
Bacteria, yeast, and microalgae serve as catalysts in producing several food components, such as enzymes and nutraceuticals. The current fad for...
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Development of probiotic E. coli Nissle 1917 for β-alanine production by using protein and metabolic engineering
Abstractβ-alanine has been used in food and pharmaceutical industries. Although Escherichia coli Nissle 1917 (EcN) is generally considered safe and...
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A programmable encapsulation system improves delivery of therapeutic bacteria in mice
Living bacteria therapies have been proposed as an alternative approach to treating a broad array of cancers. In this study, we developed a...
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Bioengineered production of glycosaminoglycans and their analogues
Glycosaminoglycans (GAGs) are a class of linear polysaccharides, consisting of alternating disaccharide sequences of uronic acid and hexosamines (or...
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Heparan Sulfate Structure: Methods to Study N-Sulfation and NDST Action
Heparan sulfate proteoglycans are important modulators of cellular processes where the negatively charged polysaccharide chains interact with target...
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Quantitative analysis of heparan sulfate using isotopically labeled calibrants
Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for...
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Microbial Engineering and Applications for the Development of Value-Added Products
Downstream is a very affluent process for fermentation. It usually involves complicated equipment and processes to obtain desired chemicals or...