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A genome-wide CRISPR screen identifies BRD4 as a regulator of cardiomyocyte differentiation
Human induced pluripotent stem cell (hiPSC) to cardiomyocyte (CM) differentiation has reshaped approaches to studying cardiac development and...
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A genome-wide screen reveals new regulators of the 2-cell-like cell state
In mammals, only the zygote and blastomeres of the early embryo are totipotent. This totipotency is mirrored in vitro by mouse ‘2-cell-like cells’...
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Genome-wide CRISPR/Cas9 screen shows that loss of GET4 increases mitochondria-endoplasmic reticulum contact sites and is neuroprotective
Organelles form membrane contact sites between each other, allowing for the transfer of molecules and signals. Mitochondria-endoplasmic reticulum...
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Genome-wide pooled CRISPR screening in neurospheres
Spheroid culture systems have allowed in vitro propagation of cells unable to grow in canonical cell culturing conditions, and may capture cellular...
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Genome-wide CRISPR screen for HSV-1 host factors reveals PAPSS1 contributes to heparan sulfate synthesis
Herpes simplex virus type 1 (HSV-1) is a ubiquitous pathogen that causes various diseases in humans, ranging from common mucocutaneous lesions to...
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A genome-wide RNAi screen for genes important for proliferation of cultured Drosophila cells at low temperature identifies the Ball/VRK protein kinase
A change in ambient temperature is predicted to disrupt cellular homeostasis by affecting all cellular processes in an albeit non-uniform manner....
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Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development
Numerous toxins threaten humans, but specific antidotes are unavailable for most of them. Although CRISPR screening has aided the discovery of the...
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Genome-Wide Expression Analysis of Genes Involved in Somatic Embryogenesis
Genome-wide expression analysis is an important tool for identifying and analysing genes involved in various biological processes, including cell...
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From a genome-wide screen of RNAi molecules against SARS-CoV-2 to a validated broad-spectrum and potent prophylaxis
Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is of utmost importance, specifically those strategies that are resistant to...
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Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescence
During this last decade, the development of prosenescence therapies has become an attractive strategy as cellular senescence acts as a barrier...
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A genome-wide screen for resilient responses in growing pigs
BackgroundThere is a growing interest to decipher the genetic background of resilience and its possible improvement through selective breeding. The...
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Identification of Genes Regulating Hepatocyte Injury by a Genome-Wide CRISPR-Cas9 Screen
Gene editing introduces stable mutations into the genome and has powerful applications extending from research to clinical gene therapy. CRISPR-Cas9...
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Genome-wide CRISPR screening identifies a role for ARRDC3 in TRP53-mediated responses
Whole-genome screens using CRISPR technologies are powerful tools to identify novel tumour suppressors as well as factors that impact responses of...
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Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry
The outbreak of coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has created a global health crisis. SARS-CoV-2 infects varieties of tissues...
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Genome-wide association study of population-standardised cognitive performance phenotypes in a rural South African community
Cognitive function is an indicator for global physical and mental health, and cognitive impairment has been associated with poorer life outcomes and...
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Genome-wide CRISPR screen identifies protein pathways modulating tau protein levels in neurons
Aggregates of hyperphosphorylated tau protein are a pathological hallmark of more than 20 distinct neurodegenerative diseases, including Alzheimer’s...
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Genome-Wide Prediction of Disease Resistance Gene Analogs in Flax
Flax (Linum usitatissimum L.) is an important crop as the sources of fiber and seed oil. Annually, various pathogens cause significant loss to flax...
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Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing
The mechanistic target of rapamycin complex 1 (mTORC1) is an essential hub that integrates nutrient signals and coordinates metabolism to control...
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Genome-wide CRISPR screen identifies synthetic lethality between DOCK1 inhibition and metformin in liver cancer
Metformin is currently a strong candidate anti-tumor agent in multiple cancers. However, its anti-tumor effectiveness varies among different cancers...
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A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens
Map** genetic interactions is essential for determining gene function and defining novel biological pathways. We report a simple to use CRISPR...
