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Optimized Nonviral Gene Disruption in Primary Murine and Human Myeloid Cells
Genetically engineered myeloid cells such as monocytes, macrophages, and dendritic cells have broad applications in basic and translational research....
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Development of an efficient ClosTron system for gene disruption in Ruminiclostridium papyrosolvens
AbstractRuminiclostridium papyrosolvens is an anaerobic, mesophilic, and cellulolytic clostridia, promising consolidated bioprocessing (CBP)...
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Disruption of Long-Distance Transport Leads to Changes in Gene Expression Profiles of Sugar Transporters in Silver Birch
AbstractKarelian birch is a form of silver birch that is characterized by a violation of the ratio and spatial orientation of the structural elements...
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Disruption of maternal vascular remodeling by a fetal endoretrovirus-derived gene in preeclampsia
BackgroundPreeclampsia, one of the most lethal pregnancy-related diseases, is associated with the disruption of uterine spiral artery remodeling...
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Effects of circadian clock disruption on gene expression and biological processes in Aedes aegypti
BackgroundThis study explores the impact of disrupting the circadian clock through a Cycle gene knockout (KO) on the transcriptome of Aedes aegypti ...
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CRISPR/Cas9 Gene Disruption Studies in F0 Xenopus Tadpoles: Understanding Development and Disease in the Frog
CRISPR/Cas9 has become the favorite method for gene knockouts in a range of vertebrate model organisms due to its ease of use and versatility....
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Disruption of RGD1 Gene Improves Acetic Acid Tolerance in Saccharomyces cerevisiae
AbstractThis study aimed to identify genes related to acetic acid tolerance and elucidate the tolerance mechanism in yeast Saccharomyces cerevisiae ...
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Targeted Gene Disruption Via CRISPR/Cas9 Ribonucleoprotein Complexes in Fusarium oxysporum
Characterization of a gene of interest frequently relies on generation of a mutant as a critical component. Transformation to disrupt a gene has been...
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Disruption of a licorice cellulose synthase-derived glycosyltransferase gene demonstrates its in planta role in soyasaponin biosynthesis
Key messageCRISPR–Cas9-mediated disruption of a licorice cellulose synthase-derived glycosyltransferase gene, GuCSyGT, demonstrated the in planta...
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Transformation and gene-disruption in the apple-pathogen, Neonectria ditissima
BackgroundApple production in Sweden and elsewhere is being threatened by the fungus, Neonectria ditissima , which causes a disease known as European...
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Targeted gene knockout via CRISPR/Cas9: precise genome editing in eggplant (Solanum melongena) through phytoene desaturase gene disruption
Genome editing using CRISPR/Cas9 has become powerful tool for enabling targeted genetic changes into crop species. The present study was aimed to...
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DEC1 is involved in circadian rhythm disruption-exacerbated pulmonary fibrosis
BackgroundThe alveolar epithelial type II cell (AT2) and its senescence play a pivotal role in alveolar damage and pulmonary fibrosis. Cell circadian...
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Validation of Lon Gene Disruption using Linear DNA Cassette by Crelox Mechanism in E. coli Strains: To Achieve Better Solubility of Putrescine Monooxygenase
Numerous expression systems, engineered strains, and cultivation systems have been developed globally but producing recombinant proteins in the...
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Nesfatin-1 and nesfatin-1-like peptide attenuate hepatocyte lipid accumulation and nucleobindin-1 disruption modulates lipid metabolic pathways
Nesfatin-1 (NESF-1) has been shown to modulate lipid metabolism. We have identified a nesfatin-1-like-peptide (NLP) processed from a related...
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Disruption of metazoan gene regulatory networks in cancer alters the balance of co-expression between genes of unicellular and multicellular origins
BackgroundMetazoans inherited genes from unicellular ancestors that perform essential biological processes such as cell division, metabolism, and...
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Maternal circadian rhythm disruption affects neonatal inflammation via metabolic reprograming of myeloid cells
Disruption of circadian rhythm during pregnancy produces adverse health outcomes in offspring; however, the role of maternal circadian rhythms in the...
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Disruption of Aokap6 near the kojic acid gene cluster affects the growth and kojic acid production in Aspergillus oryzae
The kojic acid gene cluster of Aspergillus oryzae plays a key role in kojic acid synthesis. Although the kojic acid gene cluster has been found in...
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Pair bonding and disruption impact lung transcriptome in monogamous Peromyscus californicus
Social interactions affect physiological and pathological processes, yet their direct impact in peripheral tissues remains elusive. Recently we...
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Disruption of the Uty epigenetic regulator locus in hematopoietic cells phenocopies the profibrotic attributes of Y chromosome loss in heart failure
Heart failure affects millions of people worldwide, with men exhibiting a higher incidence than women. Our previous work has shown that mosaic loss...
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Cardiomyocyte-specific disruption of the circadian BMAL1–REV-ERBα/β regulatory network impacts distinct miRNA species in the murine heart
Circadian disruption increases cardiovascular disease (CVD) risk, through poorly understood mechanisms. Given that small RNA species are critical...
