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1. Viral Gene Delivery in Chondrocytes

   Viral gene transfer, known as transduction, is a powerful research tool for studying the biology of chondrocytes in novel ways and also a technology...

   Christopher V. Nagelli, Christopher H. Evans, Rodolfo E. De La Vega in Cartilage Tissue Engineering

   Protocol 2023
   

2. Maternal Gene Delivery for the Prevention and Treatment of Obstetric Conditions

   Purpose of Review

   There is an urgent unmet clinical need for effective therapies for obstetric complications such as fetal growth restriction,...

   Sophia C. D’Alessandro, Ashley K. Boyle in Current Stem Cell Reports

   Article Open access 06 June 2024
   

3. Gene Therapeutic Delivery to the Salivary Glands

   The salivary glands, exocrine glands in our body producing saliva, can be easily damaged by various factors. Radiation therapy and Sjogren’s syndrome...

   Akshaya Upadhyay, Uyen M. N. Cao, ... Simon D. Tran in Cell Biology and Translational Medicine, Volume 20

   Chapter 2023
   

4. Nano-delivery platforms for bacterial gene transformation: suitability and challenges

   Nano-scale particles (NPs) have gained increased interest as non-viral vectors for nucleic acid delivery due to their ability to penetrate through...

   Harkamal Kaur, Anu Kalia, ... Alla Singh in International Microbiology

   Article 20 June 2024
   

5. Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing

   The CRISPR/Cas9-based gene editing system for the direct delivery of pre-assembled Cas9 ribonucleoproteins (RNPs), consisting of a Cas9 nuclease and...

   Sora Lee, Su Hyun Park, ... Cha Young Kim in Plant Biotechnology Reports

   Article 10 April 2024
   

6. Recent Advances in CRISPR/Cas9 Delivery Approaches for Therapeutic Gene Editing of Stem Cells

   Abstract

   Rapid advancement in genome editing technologies has provided new promises for treating neoplasia, cardiovascular, neurodegenerative, and...

   Malihe Lotfi, Dorsa Morshedi Rad, ... Mohammad Reza Abbaszadegan in Stem Cell Reviews and Reports

   Article Open access 18 September 2023
   

7. P26 enhances baculovirus gene delivery by modulating the mammalian antiviral response

   Abstract

   Poxins are poxviral proteins that act by degrading 2´3´-cGAMP, a key molecule of cGAS-STING axis that drives and amplifies the antiviral...

   Sabrina Amalfi, María del Pilar Plastine, ... Victoria Alfonso in Applied Microbiology and Biotechnology

   Article 14 August 2023
   

8. Surgical Methods for Cardiac Gene Delivery in Large Animals

   This chapter describes main strategies of surgical gene delivery in large animals. Existing methods of cardiac gene transfer can be classified by the...

   Michael G. Katz, Yoav Hadas, ... Efrat Eliyahu in Cardiac Gene Therapy

   Protocol 2022
   

9. Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques

   Percutaneous antegrade coronary injection is among the least invasive cardiac selective gene delivery methods. However, the transduction efficiency...

   Spyros A. Mavropoulos, Kelly P. Yamada, ... Kiyotake Ishikawa in Cardiac Gene Therapy

   Protocol 2022
   

10. Prototype mouse models for researching SEND-based mRNA delivery and gene therapy

    One of the major challenges of gene therapy—an approach to treat diseases caused by faulty genes—is a lack of technologies that deliver healthy gene...

    Channabasavaiah B. Gurumurthy, Rolen M. Quadros, Masato Ohtsuka in Nature Protocols

    Article 03 August 2022
    

11. Endobronchial Gene Delivery for Pulmonary Hypertension in a Large Animal Model

    Pulmonary hypertension (PH)Pulmonary hypertension (PH) is a devastating disease with high morbidity and mortality. Despite significant progress in...

    Olympia Bikou, Kiyotake Ishikawa in Cardiac Gene Therapy

    Protocol 2022
    

12. Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cells

    Gene editing in the murine germline is a valuable approach to investigate germ cell maturation and generate mouse models. Several studies...

    Mariella Obermeier, Vera Rogiers, ... Yoni Baert in Germ Cell Development

    Protocol 2024
    

13. Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection

    Delivery of viral vectors to the heart represents a challenging endeavor. Besides vector design, the route of substrate administration is...

    Philipp Schlegel, Philip W. J. Raake in Cardiac Gene Therapy

    Protocol 2022
    

14. Structure-guided AAV capsid evolution strategies for enhanced CNS gene delivery

    Over the past 5 years, our laboratory has systematically developed a structure-guided library approach to evolve new adeno-associated virus (AAV)...

    Trevor J. Gonzalez, Aaron Mitchell-Dick, ... Aravind Asokan in Nature Protocols

    Article 21 September 2023
    

15. Endocardial Gene Delivery Using NOGA Catheter System

    NOGANOGA/MyoStarMyostar system uses low magnetic fields and endomyocardial electrical parameters, allowing precise endomyocardial injections of...

    Satu Siimes, Niko Järveläinen, ... Seppo Ylä-Herttuala in Cardiac Gene Therapy

    Protocol 2022
    

16. Effective Viral Delivery of Genetic Constructs to Neuronal Culture for Modeling and Gene Therapy of GNAO1 Encephalopathy

    Abstract

    GNAO1 encephalopathy is an orphan genetic disease associated with early infantile epilepsy, impaired motor control, and severe developmental...

    E. A. Lunev, A. A. Shmidt, ... M. V. Bardina in Molecular Biology

    Article 29 April 2022
    

17. Genetically engineered E. coli invade epithelial cells and transfer their genetic cargo into the cells: an approach to a gene delivery system

    Purpose

    Despite advances in gene therapy, the lack of safe and efficient gene delivery systems limited the clinical effectiveness of gene therapy. Due...

    Maryam Zare, Ali Farhadi, ... Abbas Behzad Behbahani in Biotechnology Letters

    Article 11 May 2023
    

18. Stent-Based Gene Delivery for Coronary Disease

    Percutaneous coronary interventions (PCI) are the mainstay for treatment of advanced coronary disease. A majority of PCI involve deployment of a...

    Ivan S. Alferiev, Michael Chorny, ... Ilia Fishbein in Cardiac Gene Therapy

    Protocol 2022
    

19. Cardiac Gene Therapy Methods and Protocols

    This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and...

    Kiyotake Ishikawa in Methods in Molecular Biology

    Book 2022
    

20. Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy

    Recent advances in ex vivo perfusion have enabled an extended preservation time for solid organs prior to transplantation allowing for possible...

    Michelle Mendiola Pla, Amy Evans, ... Dawn E. Bowles in Cardiac Gene Therapy

    Protocol 2022