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Plant Gene Silencing Methods and Protocols
This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview...
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Gene Silencing via RNA Interference in Cryptococcus
RNA interference (RNAi) is a molecular biology technique for silencing specific eukaryotic genes without altering the DNA sequence in the genome. The...
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Genetic Analysis by Targeted Misexpression and Gene Silencing
Analysis of gene function is crucial for understanding the mechanism of biological processes. Critical clues to gene functions in vivo can be...
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CRISPRi-Mediated Gene Silencing in Biofilm Cycle and Quorum Sensing
Biofilm is an assembly of architecturally, bacterial mono-species with different functional attributes but with similar genetical makeup. It is...
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Recent Advances in Plant Gene Silencing Methods
With the increasing understanding of fundamentals of gene silencing pathways in plants, various tools and techniques for downregulating the...
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Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato
Loss-of-function analyses are essential to dissect the complex nature of biological processes, including gametogenesis. Virus-induced gene silencing...
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Transient Expression–Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors
Posttranscriptional gene silencing (PTGS) also known as RNA silencing or RNA interference is an evolutionarily conserved innate immunity in...
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A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis
DNA methylation has been utilized for target gene silencing in plants. However, it is not well understood whether other silencing pathways can be...
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Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors
As an efficient tool for functional genomics, VIGS (virus-induced gene silencing) has been widely used in reverse and forward genetics to identify...
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Production of Double-Stranded RNA in Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing
RNA silencing (also known as gene silencing) is an evolutionary conserved mechanism that is involved in regulating gene expression, suppressing...
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Spray-Induced Gene Silencing to Study Gene Function in Phytophthora
RNA interference (RNAi) is a conserved cellular defense mechanism mediated by double-stranded RNA (dsRNA) that can regulate gene expression through...
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Microbe-induced gene silencing boosts crop protection against soil-borne fungal pathogens
Small RNA (sRNA)-mediated trans-kingdom RNA interference (RNAi) between host and pathogen has been demonstrated and utilized. However, interspecies...
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Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)
Water dropwort ( Oenanthe javanica (Blume) DC), an aquatic perennial plant from the Apiaceae family, rich in dietary fibert, vitamins, and minerals....
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An atypical RNA quadruplex marks RNAs as vectors for gene silencing
The addition of poly(UG) (‘pUG’) repeats to 3′ termini of mRNAs drives gene silencing and transgenerational epigenetic inheritance in the metazoan Caen...
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Plant gene silencing signals move from the phloem to influence gene expression in shoot apical meristems
BackgroundSmall RNAs (sRNA) are potent regulators of gene expression that can diffuse short distances between cells and move long distances through...
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Characterization of a Homeodomain-Leucine Zipper Gene 12: Gene Silencing in Pepper and Arabidopsis-Based Overexpression During Abiotic Stress
Homeodomain-Leucine Zipper (HD-Zip) proteins are important ubiquitous and diverse molecular chaperones in plants. We characterized a gene CaATHB-12 ...
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An efficient virus-induced gene silencing of PDS gene in Solanum lycopersicum (cv. Rio Grande) and its functional analysis
Solanum lycopersicum L. is the second most significant fruit crop after potato. It is a rich source of various vitamins, dietary components, and...
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Enhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene
AbstractLaccase, a copper-containing polyphenol oxidase, is an important green biocatalyst. In this study, Laccase Lcc5 was homologous recombinantly...
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Silencing of a Nicotiana benthamiana ascorbate oxidase gene reveals its involvement in resistance against cucumber mosaic virus
Main conclusionSilencing of an ascorbate oxidase ( AO ) gene in N. benthamiana enhanced disease severity from cucumber mosaic virus (CMV), showing...
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SUMO protease FUG1, histone reader AL3 and chromodomain protein LHP1 are integral to repeat expansion-induced gene silencing in Arabidopsis thaliana
Epigenetic gene silencing induced by expanded repeats can cause diverse phenotypes ranging from severe growth defects in plants to genetic diseases...
