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Influence of class 2 integron integrase concentration on gene cassette insertion and excision in vivo
Integron can capture and express antimicrobial resistance gene cassettes and plays important roles in horizontal gene transfer. The establishment of...
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A Positive/Negative Selection Cassette for Red Recombination of BAC Clones
AbstractConventional gene cloning methods always require tedious processes confined by PCR conditions and restriction sites, which might result in a...
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Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette
Conditional knockout (cKO) is a genetic technique to inactivate gene expression in specific tissues or cell types in a temporally regulated manner....
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Validation of Lon Gene Disruption using Linear DNA Cassette by Crelox Mechanism in E. coli Strains: To Achieve Better Solubility of Putrescine Monooxygenase
Numerous expression systems, engineered strains, and cultivation systems have been developed globally but producing recombinant proteins in the...
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Characterization of a novel Escherichia coli recombineering selection/counterselection cassette
Recombineering is a highly efficient DNA cloning and modification technique by using the recombinase-mediated homologous recombination....
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Targeted integration in CHO cells using CRIS-PITCh/Bxb1 recombinase–mediated cassette exchange hybrid system
AbstractRecombinant Chinese hamster ovary (CHO) cell line development for complex biotherapeutic production is conventionally based on the random...
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Construction of Pseudomonas aeruginosa SDK-6 with synthetic lipase gene cassette and optimization of different parameters using response surface methodology for over-expression of recombinant lipase
Lipases are industrially important enzymes having vast applications in various fields. Cloning and expression of lipase enzyme-encoding genes in...
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Cadmium transport by mammalian ATP-binding cassette transporters
Cellular responses to toxic metals depend on metal accessibility to intracellular targets, reaching interaction sites, and the intracellular metal...
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The maize ATP-binding cassette (ABC) transporter ZmMRPA6 confers cold and salt stress tolerance in plants
Key messageZmMRPA6 was cloned and characterized as the first ATP-binding cassette (ABC) transporter in maize to be proven to participate in cold and...
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Generation of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette
Napier grass ( Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the...
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Insertion orientation within the cassette affects gene-targeting success during ends-out recombination in the yeast Saccharomyces cerevisiae
Gene-targeting is one of the most important molecular tools for genomic manipulations for research and industrial purposes. However, many factors...
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Functional Diversity of the Lepidopteran ATP-Binding Cassette Transporters
The ATP-binding cassette (ABC) transporter gene family is ubiquitous in the living world. ABC proteins bind and hydrolyze ATP to transport a myriad...
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Identification and expression analysis of ATP-binding cassette (ABC) transporters revealed its role in regulating stress response in pear (Pyrus bretchneideri)
BackgroundATP-binding cassette (ABC) transporter proteins constitute a plant gene superfamily crucial for growth, development, and responses to...
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Cassette Construction and Co-Expression of NA and F Proteins of H1N1 Influenza Virus and Newcastle Disease Virus in Saccharomyces cerevisiae
The aim of this study is to optimize the design, construction, and expression of recombinant protein, NA-F, derived from pathogenic influenza and...
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Insulin Downregulates the Expression of ATP-binding Cassette Transporter A-I in Human Hepatoma Cell Line HepG2 in a FOXO1 and LXR Dependent Manner
ATP-binding cassette transporter A-I (ABCA1) is an ubiquitously expressed protein whose main function is the transmembrane transport of cholesterol...
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The role of ATP-binding cassette transporter genes expression in treatment failure cutaneous leishmaniasis
Leishmaniasis is one of the common diseases transmitted by sand flies in tropical and subtropical regions of the world. Currently, antimonial...
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Development of a CRISPR/Cpf1 system for multiplex gene editing in Aspergillus oryzae
CRISPR/Cas technology is a powerful tool for genome engineering in Aspergillus oryzae as an industrially important filamentous fungus. Previous study...
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Functional characterization of the Komagataella phaffii 1033 gene promoter and transcriptional terminator
The methylotrophic yeast Komagataella phaffii (syn. Pichia pastoris ) is a widely used host for extracellularly producing heterologous proteins via an...
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Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome
Two Gram-stain-positive, catalase-negative, non-spore-forming, cocci-shaped strains (dk850 T and JY899) were isolated from the feces of Equus kiang in...
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Double Crossover Approach to Inactivate Target Gene in Cyanobacteria
Anabaena sp. PCC7120 (hereafter Anabaena 7120) is a nitrogen-fixing, filamentous cyanobacterium. Given its diverse metabolism, it serves as an...