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Flavins and Flavoproteins Methods and Protocols
This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality...
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Redox Titration of Flavoproteins: An Overview
Redox titration of flavoproteins allows to detect and analyze (1) the determinants of the stabilization of individual redox forms of the flavin by...
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Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Presteady State: An Application to Elucidate Oxidoreduction Mechanisms in Flavoproteins
Anaerobic stopped-flow (SF) spectrophotometry is a powerful biophysical tool that allows a complete kinetic characterization of protein interactions...
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Atomic Force Microscopy: Single-Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms
Atomic force microscopy (AFM) is one of the most versatile tools currently used in nanoscience. AFM allows for performing nondestructive imaging of...
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Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner
To perform their action usually flavoproteins interact transiently with a variety of molecular partners, whose binding is reciprocally affected and...
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Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State
To perform their action, flavoproteins usually interact with a variety of low molecular weight partners, including electron transporters, yielding...
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Insights into the catalytic mechanism of a type I sulfide quinone oxidoreductase (SQR) from Acidithiobacillus caldus
Acidithiobacillus caldus , a typical sulfur oxidizer, derives the majority of its energy from sulfur oxidation. And the essential enzyme for sulfide...
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FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics
Optical dimerizers have been developed to untangle signaling pathways, but they are of limited use in vivo, partly due to their inefficient...
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An ETFDH-driven metabolon supports OXPHOS efficiency in skeletal muscle by regulating coenzyme Q homeostasis
Coenzyme Q (Q) is a key lipid electron transporter, but several aspects of its biosynthesis and redox homeostasis remain undefined. Various...
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Inhibitors of gastric acid secretion increase oxidative stress and matrix metalloproteinase-2 activity leading to vascular remodeling
The use of inhibitors of gastric acid secretion (IGAS), especially proton pump inhibitors (PPI), has been associated with increased cardiovascular...
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Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD/Protein Monomer Ratio by UV-Vis Spectra
Here we describe a protocol for a one-step purification of a soluble form of human FAD synthase (isoform 2; hFADS2), overexpressed as a 6-His-tagged...
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Flavinated SDHA underlies the change in intrinsic optical properties of oral cancers
The molecular basis of reduced autofluorescence in oral squamous cell carcinoma (OSCC) cells relative to normal cells has been speculated to be due...
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Methacrylate Redox Systems of Anaerobic Bacteria
This review analyzes current information about the anaerobic type of respiration using a synthetic methacrylate compound as an electron acceptor....
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Quinone binding site in a type VI sulfide:quinone oxidoreductase
AbstractMonotopic membrane-bound flavoproteins, sulfide:quinone oxidoreductases (SQRs), have a variety of physiological functions, including sulfide...
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On Katsuko Kakinuma: Spectroscopic Studies of Redox Centers in NADPH Oxidase – “Identifying and Observing the Key Players that Pass an Electron to Oxygen”
Dr. Katsuko Kakinuma made many important contributions to biology and medicine through the study of the neutrophil NADPH oxidase system, particularly...
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Riboflavin Seed Priming Activates OsNHXs Expression to Alleviate Salinity Stress in Rice Seedlings
Rice possesses well-developed mechanisms of managing excessive sodium concentration under salinity stress condition. However, such mechanisms are...
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Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism
The SLC25A32 dysfunction is associated with neural tube defects (NTDs) and exercise intolerance, but very little is known about disease-specific...
