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Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines
High-resolution structure determination by electron cryo-microscopy underwent a step change in recent years. This now allows study of challenging...
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SEM: Sample Preparation, Fixation, and Staining of Protists
The ultrastructure and morphology of the protist can be studied using high-resolution electron microscope, i.e., scanning electron microscope (SEM)....
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Time of sample collection is critical for the replicability of microbiome analyses
As the microbiome field moves from descriptive and associative research to mechanistic and interventional studies, being able to account for all...
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Effect of sample size on the estimation of forest inventory attributes using airborne LiDAR data in large-scale subtropical areas
Key messageSample size (number of plots) may significantly affect the accuracy of forest attribute estimations using airborne LiDAR data in...
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Controlled Field Cage and Field Evolution
In cotton fields, predatory natural enemies play a significant role in pest control and may boost cotton growers’ profitability by lowering the need...
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Effect of training sample size, sampling design and prediction model on soil map** with proximal sensing data for precision liming
Site-specific estimation of lime requirement requires high-resolution maps of soil organic carbon (SOC), clay and pH. These maps can be generated...
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Effects of sample size, data quality, and species response in environmental space on modeling species distributions
ContextThere have been many studies using species distribution models (SDMs) to predict shifts in species distributions due to environmental changes,...
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Quantifying bias introduced by sample collection in relative and absolute microbiome measurements
To gain insight into the accuracy of microbial measurements, it is important to evaluate sources of bias related to sample condition, preservative...
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Sample Preparation and Phosphopeptide Enrichment for Plant Phosphoproteomics via Label-Free Mass Spectrometry
Phosphopeptide enrichment is the main bottleneck of every phosphorylation study. Therefore, in this chapter, a general workflow tries to overbridge...
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Differences in Characteristics of Sample Sites Explain Variable Responses of Soil Microbial Biomass to Nitrogen Addition: A Meta-Analysis
Many projects have demonstrated that excessive nitrogen (N) addition changed soil microbial biomass; however, the extent varies at different sites...
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Detection of fungi in the airways of horses according to the sample site: a methodological study
Fungal detection in equine airways may be performed on either tracheal wash (TW) or bronchoalveolar lavage fluid (BALF) by either cytology or...
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Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations
Time-resolved serial crystallography is an emerging method to elucidate the structure-function relationship of biomolecular systems at up to atomic...
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Metabolism gene expression in worker honey bees after exposure to 50Hz electric field - semi-field analysis
The investigation of the effects of artificial 50 Hz electric field (E-field) frequency on Apis mellifera is a relatively new field of research....
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Field Assessment—Survey Protocols and Data Collection
The INFC2015 field campaign surveyed almost 9000 sample points and assessed and measured a relevant number of variables. This chapter describes the...
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Rapid detection of tomato spotted wilt virus by real-time RT-LAMP and in-field application
Tomato spotted wilt virus (TSWV) is considered one of the most threatening viruses worldwide for different economically important agricultural crops....
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EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder
Main conclusionWe describe a user-optimized sample holder EasyClick for medium-sized plants that reduces root side movements and thus greatly extends...
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FruitPhenoBox – a device for rapid and automated fruit phenoty** of small sample sizes
BackgroundFruit appearance of apple ( Malus domestica Borkh.) is accession-specific and one of the main criteria for consumer choice. Consequently,...
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Sample Collection and Preparation for Annual and Intra-annual Tree-Ring Isotope Chronologies
This chapter provides guidance for conducting studies based on stable isotope measurements in tree rings to infer past and present climate...
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Ribosomal DNA copy number alteration in blood sample from gastric cancer patients
BackgroundRibosomal DNA (rDNA) is the most abundant and important housekee** gene in the cell. It usually acted as DNA damage sensor in DNA damage...
