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Directed proton transfer from Fo to F1 extends the multifaceted proton functions in ATP synthase
The role of protons in ATP synthase is typically considered to be energy storage in the form of an electrochemical potential, as well as an operating...
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ATP synthase FOF1 structure, function, and structure-based drug design
ATP synthases are unique rotatory molecular machines that supply biochemical reactions with adenosine triphosphate (ATP)—the universal “currency”,...
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Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model
The architecture of the actin cortex determines the generation and transmission of stresses, during key events from cell division to migration....
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Foam fractionation Tags (F-Tags) enabling surfactant-free, activity-preserving recovery of enzymes
AbstractEnzymes have become important tools in many industries. However, the full exploitation of their potential is currently limited by a lack of...
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RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling
Spermatogenesis is an extremely complex process, and any obstruction can cause male infertility. RhoGDIα has been identified as a risk of male...
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Different glycosylation profiles of cystatin F alter the cytotoxic potential of natural killer cells
Cystatin F, a cysteine peptidase inhibitor, is a potent modulator of NK cytotoxicity. By inhibiting granule-mediated cytotoxicity pathway, cystatin F...
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A Pseudo-near isogenic F2 population strategy for rapid QTL cloning
Near isogenic F 2 (NIF 2 ) population frequently developed by conventional backcross has dramatically contributed to QTL identification in plants....
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High resolution map** of a novel non-transgressive hybrid susceptibility locus in barley exploited by P. teres f. maculata
Hybrid genotypes can provide significant yield gains over conventional inbred varieties due to heterosis or hybrid vigor. However, hybrids can also...
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Follicular fluid C3a-peptide promotes oocyte maturation through F-actin aggregation
BackgroundImmature cumulus-oocyte complexes are retrieved to obtain mature oocytes by in vitro maturation (IVM), a laboratory tool in reproductive...
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Interaction of Venturicidin and Fo·F1-ATPase/ATP Synthase of Tightly Coupled Subbacterial Particles of Paracoccus denitrificans in Energized Membranes
AbstractProton-translocating F o ∙F 1 -ATPase/synthase that catalyzes synthesis and hydrolysis of ATP is commonly considered to be a reversibly...
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Introducing the f0% method: a reliable and accurate approach for qPCR analysis
BackgroundqPCR is a widely used technique in scientific research as a basic tool in gene expression analysis. Classically, the quantitative endpoint...
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Regulatory and evolutionary impact of DNA methylation in two songbird species and their naturally occurring F1 hybrids
BackgroundRegulation of transcription by DNA methylation in 5’-CpG-3’ context is a widespread mechanism allowing differential expression of...
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Effects of elevated CO2 and spinetoram on the population fitness and detoxification enzymes activities in Frankliniella occidentalis and F. intonsa
Herbivore performance can be impacted by elevated CO 2 or insecticides. However, there is insufficient data on how invasive and native species are...
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The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation
Binding of the mitochondrial chaperone TRAP1 to client proteins shapes bioenergetic and proteostatic adaptations of cells, but the panel of TRAP1...
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Plantlet regeneration via somatic embryogenesis and changes in endogenous hormone content of Rosa ‘John F. Kennedy’
This study describes a plantlet regeneration protocol of somatic embryos in Rosa ‘John F. Kennedy’ (hybrid tea rose). Different somatic embryo sizes...
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LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway
Long-form collapsin response mediator protein-1 (LCRMP-1) belongs to the CRMP family which comprises brain-enriched proteins responsible for axon...
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Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy
The instrumental analysis of reaction mixtures is usually the rate-determining step in the optimization of chemical processes. Traditionally,...
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Melanin Production by the Medicinal Mushroom Inonotus obliquus F-1375 in Submerged Liquid Cultivation and its Antiretroviral Properties
We have explored the effect of a new strain of the mushroom Inonotus obliquus (Ach.:Pers.) Pilat ( Basidiomycota ) F-1375 on melanin production in a...
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The F-box protein encoding genes of the leaf-rust fungi Puccinia triticina: genome-wide identification, characterization and expression dynamics during pathogenesis
The F-box proteins in fungi perform diverse functions including regulation of cell cycle, circadian clock, development, signal transduction and...
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Structural variants and tandem repeats in the founder individuals of four F2 pig crosses and implications to F2 GWAS results
BackgroundStructural variants and tandem repeats are relevant sources of genomic variation that are not routinely analyzed in genome wide association...