Search
Search Results
-
TET activity safeguards pluripotency throughout embryonic dormancy
Dormancy is an essential biological process for the propagation of many life forms through generations and stressful conditions. Early embryos of...
-
Tumor suppressor let-7 acts as a key regulator for pluripotency gene expression in Muse cells
In embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), the expression of an RNA-binding pluripotency-relevant protein, LIN28, and...
-
A multi-omics integrative analysis based on CRISPR screens re-defines the pluripotency regulatory network in ESCs
A comprehensive and precise definition of the pluripotency gene regulatory network (PGRN) is crucial for clarifying the regulatory mechanisms in...
-
Characterization of a Distinct State in the Continuum of Pluripotency Facilitated by Inhibition of PKCζ in Mouse Embryonic Stem Cells
Inhibition of PKC (PKCi) signaling maintains pluripotency of embryonic stem cells (ESCs) across different mammalian species. However, the position of...
-
Metabolic and cell cycle shift induced by the deletion of Dnm1l attenuates the dissolution of pluripotency in mouse embryonic stem cells
Mitochondria are versatile organelles that continuously change their morphology via fission and fusion. However, the detailed functions of...
-
Young LINE-1 transposon 5′ UTRs marked by elongation factor ELL3 function as enhancers to regulate naïve pluripotency in embryonic stem cells
LINE-1s are the major clade of retrotransposons with autonomous retrotransposition activity. Despite the potential genotoxicity, LINE-1s are highly...
-
Long range inter-chromosomal interaction of Oct4 distal enhancer loci regulates ESCs pluripotency
Nuclear architecture underlies the transcriptional programs within the cell to establish cell identity. As previously demonstrated, long-range...
-
NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency
Human NANOG expression resets stem cells to ground-state pluripotency. Here we identify the unique features of human NANOG that relate to its...
-
Induction of Human Naïve Pluripotency Using 5i/L/A Medium
Prior to implantation, the cells in the mammalian epiblast constitute a naïve pluripotent state, which is distinguished by absence of lineage...
-
m6A RNA methylation orchestrates transcriptional dormancy during paused pluripotency
Embryos across metazoan lineages can enter reversible states of developmental pausing, or diapause, in response to adverse environmental conditions....
-
Dissecting OCT4 defines the role of nucleosome binding in pluripotency
Pioneer transcription factors such as OCT4 can target silent genes embedded in nucleosome-dense regions. How nucleosome interaction enables...
-
Pluripotency Stemness and Cancer: More Questions than Answers
Embryonic stem cells and induced pluripotent stem cells provided us with fascinating new knowledge in recent years. Mechanistic insight into...
-
Restricting epigenetic activity promotes the reprogramming of transformed cells to pluripotency in a line-specific manner
Somatic cell reprogramming and oncogenic transformation share surprisingly similar features, yet transformed cells are resistant to reprogramming....
-
The role of BMP4 signaling in trophoblast emergence from pluripotency
The Bone Morphogenetic Protein (BMP) signaling pathway has established roles in early embryonic morphogenesis, particularly in the epiblast. More...
-
Modaline sulfate promotes Oct4 expression and maintains self-renewal and pluripotency of stem cells through JAK/STAT3 and Wnt signaling pathways
BackgroundStem cells have been extensively explored for a variety of regenerative medical applications and they play an important role in clinical...
-
c-MYC Triggers Lipid Remodelling During Early Somatic Cell Reprogramming to Pluripotency
Metabolic rewiring and mitochondrial dynamics remodelling are hallmarks of cell reprogramming, but the roles of the reprogramming factors in these...
-
Establishing extended pluripotent stem cells from human urine cells
BackgroundExtended pluripotent stem cells (EPSCs) can contribute to both embryonic and trophectoderm-derived extraembryonic tissues. Therefore, EPSCs...
-
DNA methylation restricts coordinated germline and neural fates in embryonic stem cell differentiation
As embryonic stem cells (ESCs) transition from naive to primed pluripotency during early mammalian development, they acquire high DNA methylation...
-
Glis1 facilitates induction of pluripotency via an epigenome–metabolome–epigenome signalling cascade
Somatic cell reprogramming provides insight into basic principles of cell fate determination, which remain poorly understood. Here we show that the...
-
Global hyperactivation of enhancers stabilizes human and mouse naive pluripotency through inhibition of CDK8/19 Mediator kinases
Pluripotent stem cells (PSCs) transition between cell states in vitro, reflecting developmental changes in the early embryo. PSCs can be stabilized...
