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Isolated microspore culture for embryoid production in Artemisia annua L.
The haploidy technique is a useful tool for quickly producing pure, fully homozygous lines. Artemisia annua L. is a medicinal plant that produces...
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The role of c-Jun for beating cardiomycyte formation in prepared embryonic body
BackgroundMorbidity and mortality associated with cardiovascular diseases, such as myocardial infarction, stem from the inability of terminally...
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Exosomes induce neurogenesis of pluripotent P19 cells
Exosomes play a role in tissue/organ development and differentiation. Retinoic acid induces differentiation of P19 cells (UD-P19) to P19 neurons...
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Stem Cell Differentiation into Cardiomyocytes: Current Methods and Emerging Approaches
AbstractCardiovascular diseases (CVDs) are globally known to be important causes of mortality and disabilities. Common treatment strategies for CVDs,...
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Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Despite numerous efforts to generate vascular tissues that recapitulate the physiological characteristics of native vessels, vascular cell source...
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Macro- and Micropropagation of Plants for Income Generation
The present chapter focused on the development of the macro–micro multiplication system of three economically important plants in silviculture...
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Derivation of Induced Pluripotent Stem Cell (iPSC) Lines from Patient-Specific Peripheral Blood Mononuclear Cells (PBMC) Using Episomal Vectors
Inherited retinal diseases (IRDs) are a diverse group of rare eye disorders, resulting in vision loss or blindness. The underlying reason is mutation...
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Stochastic biological system-of-systems modelling for iPSC culture
Large-scale manufacturing of induced pluripotent stem cells (iPSCs) is essential for cell therapies and regenerative medicines. Yet, iPSCs form large...
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Trim28 citrullination maintains mouse embryonic stem cell pluripotency via regulating Nanog and Klf4 transcription
Protein citrullination, including histone H1 and H3 citrullination, is important for transcriptional regulation, DNA damage response, and...
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A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells
Human pluripotent stem cells (hPSCs) are capable of extensive self-renewal yet remain highly sensitive to environmental perturbations in vitro,...
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CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells
The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF...
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An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype
BackgroundAssociation of chromatin with lamin proteins at the nuclear periphery has emerged as a potential mechanism to coordinate cell type-specific...
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Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters
The inability to scalably and precisely measure the activity of developmental cis -regulatory elements (CREs) in multicellular systems is a bottleneck...
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Cerebral organoids display dynamic clonal growth and tunable tissue replenishment
During brain development, neural progenitors expand through symmetric divisions before giving rise to differentiating cell types via asymmetric...
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Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
BackgroundThe progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for...
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Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications
Long-term neuroepithelial-like stem cells (lt-NES) derived from human embryonic stem cells are a stable self-renewing progenitor population with high...
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Analysis of Queuosine tRNA Modification Using APB Northern Blot Assay
Queuosine (Q) is a hypermodified base that occurs at the wobble position of transfer RNAs (tRNAs) with a GUN anticodon. Q-tRNA modification is...
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Single-cell landscape of nuclear configuration and gene expression during stem cell differentiation and X inactivation
BackgroundMammalian development is associated with extensive changes in gene expression, chromatin accessibility, and nuclear structure. Here, we...
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GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells
BackgroundA major challenge for the clinical use of human pluripotent stem cells is the development of safe, robust and controlled differentiation...
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Epiblast fragmentation by shedding—a novel mechanism to eliminate cells in post-implantation mouse embryos
The role of programmed cell death during embryonic development has been described previously, but its specific contribution to peri- and...
