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Molecular detection of Paracoccidioides spp. in environmental samples from the Southwestern Amazon
Paracoccidioidomycosis is an infection with the potential for environmental dissemination, especially in regions of hot and humid climate, where...
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Validating post-enrichment steps in environmental RNA analysis for improving its availability from water samples
Environmental RNA (eRNA) analysis is expected to inclusively provide the physiological information of a population and community without individual...
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Detection of African swine fever virus and wild boar eDNA in soil and turbid water samples: towards environmental surveillance
Since 2007, an ongoing African swine fever (ASF) pandemic has significantly impacted Eurasia. Extensive field evidence and modeling confirm the...
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Assessment of fungal spores and spore-like diversity in environmental samples by targeted lysis
At particular stages during their life cycles, fungi use multiple strategies to form specialized structures to survive unfavorable environmental...
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Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples
A probe-based quantitative real-time PCR assay was developed to detect meltwater stonefly ( Lednia tumana ) environmental (e)DNA in water samples. The...
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Campylobacter prevalence from food, animals, human and environmental samples in Iran: a systematic review and meta-analysis
BackgroundCampylobacter regarded as a major cause of foodborne gastroenteritis in humans. The present study aimed to determine the prevalence of campylobacter...
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Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples
We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander ( Ambystoma gracile ), a...
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Design and Validation of Primer Sets for the Detection and Quantification of Antibiotic Resistance Genes in Environmental Samples by Quantitative PCR
The high prevalence of antibiotic resistant bacteria (ARB) in several environments is a great concern threatening human health. Particularly,...
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Survival of Streptococcus equi subsp. zooepidemicus on environmental samples is affected by material type and incubation temperature
Streptococcus equi subsp. zooepidemicus is an opportunistic pathogen associated with disease in a range of domestic and wild animals. Despite its...
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Diversity of Antibiotic Resistance genes and Transfer Elements-Quantitative Monitoring (DARTE-QM): a method for detection of antimicrobial resistance in environmental samples
Effective monitoring of antibiotic resistance genes and their dissemination in environmental ecosystems has been hindered by the cost and efficiency...
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Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans
Mycobacterium ulcerans is a slow-growing environmental bacterium that causes a severe skin disease known as Buruli ulcer. Identification of...
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Extraction of Microbial Cells from Environmental Samples for FISH Approaches
Fluorescent in situ hybridization (FISH) on environmental samples has become a standard technique to identify and enumerate microbial populations....
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Environmental DNA Isolation, Validation, and Preservation Methods
Environmental DNA (eDNA) workflows contain many familiar molecular-lab techniques, but also employ several unique methodologies. When working with...
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Assessing the microcystins concentration through optimized protein phosphatase inhibition assay in environmental samples
Protein phosphatase (PPase) inhibition assay (PPIA) is widely used to analyze the concentration of microcystins (MCs) because it is comparatively...
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Whole genome sequencing and taxonomic profiling of two Pantoea sp. isolated from environmental samples in Israel
ObjectiveAs part of a research aiming at the isolation of bacteria secreting growth inhibiting compounds, cultures of Francisella tularensis were...
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Environmental effects on faecal genoty** success in mesocarnivores
Non-invasive genetic sampling can be used in research, monitoring, and conservation of wild animals to, for example, provide insights into diets,...
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Prokaryotic diversity and potentially pathogenic bacteria in vended foods and environmental samples
PurposeReady-to-eat fast food vending outlets provide a cheap and readily available food. Foodborne diseases have been previously reported in Embu,...
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Evaluation of Environmental and Nutritional Aspects of Bee Pollen Samples Collected from East Black Sea Region, Turkey, via Elemental Analysis by ICP-MS
AbstractHoneybee pollens are good food sources in terms of their mineral contents and are specific to the regions they are collected. In addition,...
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Molecular Techniques and Technologies in Biomonitoring for Environmental Sustainability
For all living things, including humans, to continue existing, the environment plays a crucial role. Microbiological and molecular biology...