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Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study
Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and...
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3D Cell Culture: Techniques For and Beyond Organoid Applications
In the rapidly evolving landscape of cell biology and biomedical research, three-dimensional (3D) cell culture has contributed not only to the...
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Development of Cell Culture Platforms for Study of Trabecular Meshwork Cells and Glaucoma Development
Background:Various cell culture platforms that could display native environmental cue-mimicking stimuli were developed, and effects of environmental...
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Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy
To produce abundant cell culture samples to generate large, standardized image datasets of human induced pluripotent stem (hiPS) cells, we developed...
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Intestinal Cell Differentiation and Phenotype in 2D and 3D Cell Culture Models
Three-dimensional (3D) culture models are more physiologically relevant than two-dimensional (2D) cell culture models. 2D approaches cannot reproduce...
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Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds
In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds...
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Tumor-Microenvironment-on-Chip Platform for Assessing Drug Response in 3D Dynamic Culture
Microphysiological systems involving microfluidic 3D culture of cancer cells have emerged as a versatile toolkit to study tumor biological problems...
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Application of three-dimensional cell culture technology in screening anticancer drugs
The drug development process involves a variety of drug activity evaluations, which can determine drug efficacy, strictly analyze the biological...
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Quantitative Image Analysis of Dynamic Cell Behaviors During Border Cell Migration
Drosophila border cells have emerged as a genetically tractable model to investigate dynamic collective cell migrationMigrations within the context...
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Advances in Characterization of Industrial Bioreactors for Cell Culture Process
Aerated stirred tank reactors are widely used in the biopharmaceutical industry for cell culture applications due to their flexibility and broad...
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Bringing cell biology into classroom: tips to culture and observe skeletal muscle cells in high school and college
Watching living cells through a microscope is much more exciting than seeing pictures of cells in high school and college textbooks. However,...
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Development of a 48-Well Dynamic Suspension Culture System for Pancreatic Differentiation from Human Embryonic Stem Cells
BackgroundHuman pluripotent stem cells (hPSCs) have started to emerge as a potential tool with application in fields of drug discovery, disease...
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A mutant methionyl-tRNA synthetase-based toolkit to assess induced-mesenchymal stromal cell secretome in mixed-culture disease models
BackgroundMesenchymal stromal cells (MSCs) have a dynamic secretome that plays a critical role in tissue repair and regeneration. However, studying...
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Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity
BackgroundNeoplastic subpopulations can include polyploid cells that can be involved in tumor evolution and recurrence. Their origin can be traced...
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Fibroblast-Epithelium Co-culture Methods Using Epithelial Organoids and Cell Line–Derived Spheroids
Fibroblasts are an integral cell type of mammary gland stroma, which plays crucial roles in development, homeostasis, and tumorigenesis of mammary...
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Identification of a clinically efficacious CAR T cell subset in diffuse large B cell lymphoma by dynamic multidimensional single-cell profiling
Chimeric antigen receptor (CAR) T cells used for the treatment of B cell malignancies can identify T cell subsets with superior clinical activity....
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Aggregates’ Formation in Higher Plants’ Cell Culture: The Role of Cell Wall Components
AbstractThe use of higher plant cell cultures for the production of recombinant proteins, including medical ones, seems to be a promising alternative...
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Dynamic YAP expression in the non-parenchymal liver cell compartment controls heterologous cell communication
IntroductionThe Hippo pathway and its transcriptional effectors yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif...
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A single-cell atlas of lung homeostasis reveals dynamic changes during development and aging
Aging is a global challenge, marked in the lungs by function decline and structural disorders, which affects the health of the elderly population. To...
