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Eukaryotic-driven directed evolution of Cas9 nucleases
BackgroundFurther advancement of genome editing highly depends on the development of tools with higher compatibility with eukaryotes. A multitude of...
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In vitro generation of genetic diversity for directed evolution by error-prone artificial DNA synthesis
Generating genetic diversity lies at the heart of directed evolution which has been widely used to engineer genetic parts and gene circuits in...
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Directed-evolution mutations enhance DNA-binding affinity and protein stability of the adenine base editor ABE8e
Adenine base editors (ABEs), consisting of CRISPR Cas nickase and deaminase, can chemically convert the A:T base pair to G:C. ABE8e, an evolved...
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Virus-assisted directed evolution of enhanced suppressor tRNAs in mammalian cells
Site-specific incorporation of unnatural amino acids (Uaas) in living cells relies on engineered aminoacyl-transfer RNA synthetase–tRNA pairs...
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Directed evolution of tripartite ATP-independent periplasmic transporter for 3-Hydroxypropionate biosynthesis
AbstractOur previous study’s introduction of the malonic acid assimilation pathway into Escherichia coli enabled biosynthesis of 3-Hydroxypropionate...
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Improvement of thermoalkaliphilic laccase (CtLac) by a directed evolution and application to lignin degradation
AbstractThermoalkaliphilic laccase (CtLac) from the Caldalkalibacillus thermarum strain TA2.A1 has advantageous properties with potential industrial...
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Methanol tolerance upgrading of Proteus mirabilis lipase by machine learning-assisted directed evolution
For many crucial industrial applications, enzyme-catalyzed processes take place in harsh organic solvent environments. However, it remains a...
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Directed yeast genome evolution by controlled introduction of trans-chromosomic structural variations
Naturally occurring structural variations (SVs) are a considerable source of genomic variation that can reshape the 3D architecture of chromosomes....
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Directed evolution of the PobR allosteric transcription factor to generate a biosensor for 4-hydroxymandelic acid
Hydroxy-mandelic acid (HMA) is widely applied in pharmaceuticals, food and cosmetics. In this study, we aimed to develop an allosteric transcription...
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Directed evolution in mammalian cells
Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast—even when the goal is to probe or modulate...
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Directed evolution of adeno-associated virus for efficient gene delivery to microglia
As the resident immune cells in the central nervous system (CNS), microglia orchestrate immune responses and dynamically sculpt neural circuits in...
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Engineering complex communities by directed evolution
Directed evolution has been used for decades to engineer biological systems at or below the organismal level. Above the organismal level, a small...
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Development of MEMS directed evolution strategy for multiplied throughput and convergent evolution of cytochrome P450 enzymes
Directed evolution (DE) inspired by natural evolution (NE) has been achieving tremendous successes in protein/enzyme engineering. However, the...
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Directed mutagenesis in plants through genome editing using guide RNA library
Plants with genetic diversity have a higher rate of resilience and adaptation to environmental change. Crop species need to diversify genetically if...
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Directed evolution of a wax ester synthase for production of fatty acid ethyl esters in Saccharomyces cerevisiae
AbstractWax ester synthases (WSs) utilize a fatty alcohol and a fatty acyl-coenzyme A (activated fatty acid) to synthesize the corresponding wax...
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Directed evolution and selection of biostable l-DNA aptamers with a mirror-image DNA polymerase
Mirror-image aptamers made from chirally inverted nucleic acids are nuclease-resistant and exceptionally biostable, opening up opportunities for...
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A step forward to the optimized HlyA type 1 secretion system through directed evolution
AbstractSecretion of proteins into the extracellular space has great advantages for the production of recombinant proteins. Type 1 secretion systems...
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Directed evolution of glycosyltransferase for enhanced efficiency of avermectin glucosylation
AbstractAvermectin, produced by Streptomyces avermitilis , is an active compound protective against nematodes, insects, and mites. However, its...
