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Interaction of Mesenchymal Stromal Cells with 5XFAD Mouse Hippocampal Cells in Primary Culture Depending on Cocultivation Method
Abstract—We studied the mutual influence of multipotent mesenchymal stromal cells (MMSC) isolated from human umbilical cord Wharton’s jelly and...
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Cultivation of primary cells derived from three organs of a striped dolphin (Stenella coeruleoalba) using a simple culture method
Cetacean-cultured cells are a promising tool for life science research. Most cells used in cetacean research are derived from the skin and kidneys....
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Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana
There is an expanding need to modify plant genomes to create new plant germplasm that advances both basic and applied plant research. Most current...
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Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method
Cancer immunotherapy with autologous chimeric antigen receptor (CAR) T cells faces challenges in manufacturing and patient selection that could be...
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Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment
The therapeutic effects of extracellular vesicles (EVs) have been identified as a significant factor in intercellular communication in different...
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Pharmacological Properties and Tissue Culture Method of Endangered Medicinal Plants
There are 11,146 species of medicinal plants in China, accounting for about one-third of all plant species in the country. These rare and endangered...
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Direct Shoot From Root and True-to-Type Micropropagation of Limonium “Misty Blue” in Partially Immersed Culture on an Aluminum Mesh Raft
Commercial plant tissue culture now primarily serves the ornamental horticulture industry. The main pillars of the commercial tissue culture business...
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Developmental Toxicity Using the Rat Whole Embryo Culture
In vivo and in vitro experiments have been used to investigate the effect of drugs, chemical agents, growth factors, vitamins, etc., on embryonic...
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Agrobacterium-mediated in planta transformation of cut coleoptile: a new, simplified, and tissue culture-independent method to deliver the CRISPR/Cas9 system in rice
BackgroundAgrobacterium -mediated transformation and particle bombardment are the two common approaches for genome editing in plant species using...
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Cell Culture Laboratory
The first objective of cell culture laboratory is to maintain hygiene and keep it contamination-free. The design of a cell culture laboratory majorly...
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A culture method with berbamine, a plant alkaloid, enhances CAR-T cell efficacy through modulating cellular metabolism
Memory T cells demonstrate superior in vivo persistence and antitumor efficacy. However, methods for manufacturing less differentiated T cells are...
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Comparison of novel rapid diagnostic of blood culture identification and antimicrobial susceptibility testing by Accelerate Pheno system and BioFire FilmArray Blood Culture Identification and BioFire FilmArray Blood Culture Identification 2 panels
BackgroundConventional turnaround time (TAT) for positive blood culture (PBC) identification (ID) and antimicrobial susceptibility testing (AST) is...
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An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants
BackgroundThe development of the plant in vitro techniques has brought about the variation identified in regenerants known as somaclonal or tissue...
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In Vitro Culture of Arabidopsis Embryos
Arabidopsis thaliana is currently the most important model organism for basic molecular plant research. It is also a favourable model for...
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Comparison of six anther culture methods for the production of doubled haploids in eggplant (Solanum melongena L.)
Eggplant ( Solanum melongena L.) is one of the most produced and high value vegetable crop in the world. Hybrid breeding toward high yielding variety...
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Liver organoid culture methods
Organoids, three-dimensional structures cultured in vitro, can recapitulate the microenvironment, complex architecture, and cellular functions of in...
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Plant Cell and Tissue Culture: Propagation, Improvement, and Conservation of Medicinal Plants
Plant tissue culture, a revolutionary technique in plant biology, is a highly effective method for multiplying, biodiversity conservation, and...
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An untargeted metabolomics approach to study changes of the medium during human cornea culture
IntroductionTwo main approaches (organ culture and hypothermia) for the preservation and storage of human donor corneas are globally adopted for...
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Decolorization of Crystal Violet by a Mixed Culture under the Influence of Bioelectrochemical Stimulation
AbstractA significant variation in the relative representation of copies of bacterial genes of dye-decolorizing DyP peroxidases typical for the genus
