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Denaturing Gradient Gel Electrophoresis (DGGE) Analysis of the Fungi Involved in Biodegradation
Tracing fungal communities through denaturing gradient gel electrophoresis (DGGE) is an incredibly affordable, exploratory, and qualitative molecular...
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Methylation-Specific Electrophoresis
Methylation of CpG sites in the promoter region of genomic DNA is an important epigenetic modification that plays a critical role in gene regulation,...
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Polyacrylamide Gel Electrophoresis
Polyacrylamide Gel Electrophoresis is based on the principle of migration of charged particles under the influence of electric field to separate out...
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Denaturing Gradient Gel Electrophoresis (DGGE) to Estimate Fungal Diversity
Assessments of fungal communities based on spores released from decaying leaves miss non-sporulating mycelia. Molecular approaches circumvent this...
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Denaturing gradient gel electrophoresis and multi-SIR profiles of soil microbial communities from a karst doline at Aggtelek National Park, Hungary
Soils play an important role in the ecosystem of karstic landscapes both as a buffer zone and as a source of acidity to belowground water. Although...
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Electrophoresis, a transport technology that transitioned from moving boundary method to zone method
Gel electrophoresis, a transport technology, is one of the most widely used experimental methods in biochemical and pharmaceutical research and...
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Use of PCR-DGGE-Based Molecular Methods to Analyze Nematode Community Diversity
DGGE (denaturing gradient gel electrophoresis) is a nucleic acid separation technique applied to the evaluation of microbial biodiversity. This...
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Plant-Nematode Interactions
This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their...
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In-Gel Cyanoethylation for Pseudouridines Mass Spectrometry Detection of Bacterial Regulatory RNA
Regulatory RNAs, as well as many RNA families, contain chemically modified nucleotides, including pseudouridines (ψ). To map nucleotide...
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Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA
Faithful expression of the mitochondrial genome is required for the synthesis of the oxidative phosphorylation complexes and cell fitness. In humans,...
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Rapid Genoty** of Campylobacter coli Strains from Poultry Meat by PFGE, Sau-PCR, and fla-DGGE
The genoty** of Campylobacter coli was done using three methods, pulsed-field gel electrophoresis (PFGE), Sau-polymerase chain reaction (Sau-PCR),...
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Uncultivable Soil Microbes Contributing to Sustainable Agriculture
The science of microbial genomics and metagenomics is necessary to understand the complete microbial composition in soil, water, and other...
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Reveal of Gut Microbiota of Nymphs Ameletus inopinatus (Eaton), 1887 (Ephemeroptera: Ameletidae) with Molecular and Microbiological Analyzes
AbstractMayflies, recognized for their hemimetabolous life cycle and ephemeral adult phase, exhibit diverse larval forms categorized as burrowing,...
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Use of Native-PAGE for the Identification of Epichaperomes in Cell Lines
Epichaperomes are disease-associated pathologic scaffolds, composed of tightly bound chaperones, co-chaperones, and other factors. They mediate...
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Molecular Tools for Monitoring and Validating Bioremediation
BioremediationBioremediation may be defined as the mechanism by which a biological agent (e.g., microbesMicrobes, fungiFungi, plants, and...
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Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE
BackgroundThe accurate and expeditious detection of SARS-CoV-2 mutations is critical for monitoring viral evolution, assessing its impact on...
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In Vitro Assay to Measure APE1 Enzymatic Activity on Ribose Monophosphate Abasic Site
APE1 (apurinic/apyrimidinic endodeoxyribonuclease 1) is a central enzyme of the base excision repair (BERBase excision repair (BER)) pathway playing...
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Bacteria consortia enhanced hydrocarbon degradation of waxy crude oil
Waxy crude oil is a problem to the oil and gas industry because wax deposition in pipelines reduces the quality of the crude oil. Currently, the...
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Analysis of the bacterial diversity in Moroccan Jben cheese using TTGE, DGGE, and 16S rRNA sequencing
This research investigated the physicochemical, microbiological, and bacterial diversity of Jben cheese, a popular artisanal variety in Morocco. The...
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Oxidized Low-Density Lipoprotein: Preparation, Validation, and Use in Cell Models
Lipoproteins in plasma are constituted by the least dense chylomicron, very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and...