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Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as...
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Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum
BackgroundThe native potatoes ( Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to...
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Correlative SIP-FISH-Raman-SEM-NanoSIMS links identity, morphology, biochemistry, and physiology of environmental microbes
Microscopic and spectroscopic techniques are commonly applied to study microbial cells but are typically used on separate samples, resulting in...
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Microscopy-Based Studies
Successful colonization of plants by endophytes is the basic requirement for plant-microbe interactions. Thus, the study of plant colonization routes...
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Effects of Bacillus in Pectobacterium quorum quenching: A survey of two different acyl-homoserine lactonases
Numerous functions in pathogenic Pectobacterium are regulated by quorum sensing (QS). Two different aiiA genes isolated from Bacillus sp. A24( aiiA A24 )...
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Selective deforestation and exposure of African wildlife to bat-borne viruses
Proposed mechanisms of zoonotic virus spillover often posit that wildlife transmission and amplification precede human outbreaks. Between 2006 and...
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Advantages and Limitations of the Biofilm Study Methods
AbstractA community of microorganisms adhered to a solid surface and entrapped in a self-produced extracellular polymeric matrix, in general is...
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Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling
Label-free molecular imaging techniques such as matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) enable the direct...
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FISH Variants
FISH has gained an irreplaceable place in microbiology because of its ability to detect and locate a microorganism, or a group of organisms, within...
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Phyllosphere symbiont promotes plant growth through ACC deaminase production
Plant growth promoting bacteria can confer resistance to various types of stress and increase agricultural yields. The mechanisms they employ are...
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Anaerobic methanotroph ‘Candidatus Methanoperedens nitroreducens’ has a pleomorphic life cycle
‘ Candidatus Methanoperedens’ are anaerobic methanotrophic (ANME) archaea with global importance to methane cycling. Here meta-omics and fluorescence...
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RNA-Based Vaccines and Therapeutics Against Intracellular Pathogens
RNA-based vaccines have sparked a paradigm shift in the treatment and prevention of diseases by nucleic acid medicines. There has been a notable...
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Zebrafish (Danio rerio) Oatp2b1 as a functional ortholog of the human OATP2B1 transporter
OATP2B1 belongs to a highly conserved organic anion transporting polypeptide (OATP) family of transporters, involved in the cellular uptake of both...
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Soil Health Management and Microorganisms: Recent Development
One of the essential components for sustaining life on Earth is soil. It provides a diverse range of ecosystem services that are supported by soil...
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Membrane surfaces regulate assembly of ribonucleoprotein condensates
Biomolecular condensates organize biochemistry, yet little is known about how cells control the position and scale of these structures. In cells,...
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The biogeography of infection revisited
Many microbial communities, including those involved in chronic human infections, are patterned at the micron scale. In this Review, we summarize...
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Integrating novel tools to elucidate the metabolic basis of microbial symbiosis in reef holobionts
Microorganisms form symbiotic relationships with the majority of animals and plants on earth and are integral to their health and functioning in the...
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Assigning Function to Phylogeny: FISH-nanoSIMS
Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) is an imaging method used to identify microorganisms in environmental...
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Long non-coding RNA SNHG6 couples cholesterol sensing with mTORC1 activation in hepatocellular carcinoma
Cholesterol contributes to the structural basis of biological membranes and functions as a signaling molecule, whose dysregulation has been...
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MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological map** of live cells
Map** 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such...
