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DNA-free genome editing in tomato protoplasts using CRISPR/Cas9 ribonucleoprotein delivery
CRISPR/Cas9 ribonucleoproteins enable DNA-free genome editing; thus, improving protoplast culture is essential for the efficient development of...
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Genome Editing in Forest Trees
Since the first CRISPR/Cas-mediated genome editing of poplar in 2015, an increasing number of tree species are being genome-edited. Although the...
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Strategies to improve genome editing efficiency in crop plants
Genome editing technology comprises site-directed mutagenesis of genomes, involving alterations of few bases to precise replacement of a fragment or...
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Genome Editing in Biotech Regulations Worldwide
Since the introduction of genome editing techniques in breeding and the first commercial products on the market, various governments or jurisdictions...
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Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum)
Key messageWe have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant...
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Precision Genome Editing with CRISPR-Cas9
The CRISPR/Cas9 system is a revolutionary technology for genome editing that allows for precise and efficient modifications of DNA sequences. The...
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Genome-Editing Technologies in Crop Improvement
Global climate change, together with harmful biotic and abiotic factors, is limiting agricultural yields, rendering it challenging to meet the rising...
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Plant Genome Editing Technologies: An Updated Overview
A genome is a representation of all the genetic material that forms an organism and is stored in its deoxyribonucleic acid (DNA). Genetic...
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Genome Editing of Silkworms
Silkworm is a lepidopteran insect that has been used as a model for a wide variety of biological studies. The microinjection technique is available,...
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Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing
The CRISPR/Cas9-based gene editing system for the direct delivery of pre-assembled Cas9 ribonucleoproteins (RNPs), consisting of a Cas9 nuclease and...
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Genome Editing of Gene Families for Crop Improvement
Crop improvement has been a long-standing focus of agricultural research, aiming to enhance nutritional richness, aroma, visual appeal, and yield to...
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Recent Trends in Genome Editing Technologies for Agricultural Crop Improvement
Growing world population, expanding demand of biofuels, and decrease in arable land have reduced the production of agriculture comedies. Precisely,...
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Potato Genome Editing: Recent Challenges and a Practical Procedure
Potato (Solanum tuberosum L.) is one of the most important staple crops, accounting for the fourth highest production in the world. To date, many...
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Genome Editing of Pig
Pigs have anatomical and physiological characteristics similar to humans; therefore, genetically modified pigs have the potential to become a...
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Plant Breeding Becomes Smarter with Genome Editing
The conventional concept of plant breeding as art and science is shifting toward less art and more science due to the rapid development in genomics,...
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Genome editing in peanuts: advancements, challenges and applications
Genome editing, a powerful technology for precise manipulation of DNA sequences, has revolutionized the field of agricultural biotechnology. In...
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Engineering Phytonutrient Content in Tomato by Genome Editing Technologies
The application of precise genome editing represents an important step-forward in plant functional genomics research and crop improvement by...
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Novel Delivery Methods for CRISPR-Based Plant Genome Editing
Using modern genome editing tools, scientists are increasingly able to engineer animals and plants for better traits and improved downstream outcomes...
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Background: Genome Editing with Programmable Nucleases
Scientists use genome editing with programmable nucleases to carry out the targeted modification of nucleic acid. The major genome editing...
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Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology
Modern genome editing tools particularly CRISPR/Cas9 have revolutionized plant genome manipulation for engineering resilience against changing...