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Interstrand crosslinking of homologous repair template DNA enhances gene editing in human cells
We describe a strategy to boost the efficiency of gene editing via homology-directed repair (HDR) by covalently modifying the template DNA with...
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Targeted genome editing with a DNA-dependent DNA polymerase and exogenous DNA-containing templates
Reverse transcriptases, used in prime editing systems, exhibit lower fidelity, processivity and dNTP affinity than many DNA-dependent DNA...
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From Template Principle of Nikolai K. Koltzov to the Double Helix Model of DNA Structure
AbstractThe “Omnis molecule ex molecule” idea of the template principle for the hereditary molecule’s replication is one of the most significant...
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Fast and efficient template-mediated synthesis of genetic variants
Efficient methods for the generation of specific mutations enable the study of functional variations in natural populations and lead to advances in...
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Template-Assisted Assembly of DNA Nanostructures from Branched Oligonucleotides
AbstractWe have proposed previously a template-assisted method for 3D assembly of nucleic acid nanostructures. The method involved a branched...
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DNA Sequencing Technologies and DNA Barcoding
DNA barcodes are short, standardized DNA segments that geneticists can use to identify all living taxa. On the other hand, DNA barcoding identifies...
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The plant organellar primase-helicase directs template recognition and primosome assembly via its zinc finger domain
BackgroundThe mechanisms and regulation for DNA replication in plant organelles are largely unknown, as few proteins involved in replisome assembly...
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Template-independent enzymatic synthesis of RNA oligonucleotides
RNA oligonucleotides have emerged as a powerful therapeutic modality to treat disease, yet current manufacturing methods may not be able to deliver...
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Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies
Microbial communities play an important role in marine ecosystem processes. Although the number of studies targeting marker genes such as the 16S...
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Mutation Knock-in Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish
Introduction or knock-in of precise genomic modifications remains one of the most important applications of CRISPR/Cas9 in all model systems...
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Introduction and Perspectives of DNA Base Editors
DNA base editors, one of the CRISPR-based genome editing tools, can induce targeted point mutations at desired sites. Their superiority is based on...
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A template wizard for the cocreation of machine-readable data-reporting to harmonize the evaluation of (nano)materials
Making research data findable, accessible, interoperable and reusable (FAIR) is typically hampered by a lack of skills in technical aspects of data...
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Recombinant DNA Technology
The concept of recombinant DNA (rDNA) technology was developed after the discovery of restriction enzymes by Werner Arber in bacteria that degrade...
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DNA-Replikation
Die Replikation sorgt für eine Verdopplung der DNA und ist ein grundlegender Vorgang in allen Zellen. Sie gewährleistet, dass bei der Weitergabe des...
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Simple, Robust Invertebrate DNA Barcoding: Chelex-Based DNA Extraction and Optimized COI Amplification
Chelex-based DNA extractions are well suited for student DNA barcoding research because they are simple, safe, and inexpensive and can be performed...
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Chromatin Modifications in DNA Repair
A requirement of nuclear processes that use DNA as a substrate is the manipulation of chromatin in which the DNA is packaged. Chromatin...
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DNA Barcoding Techniques for Protists
The study of free-living protist populations in soil is gaining momentum due to their crucial role in soil ecosystems, particularly in carbon and...
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Molecular Cloning Using In Vivo DNA Assembly
Here we describe the in vivo DNA assembly approach, where molecular cloning procedures are performed using an E. coli recA-independent recombination...
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Aberrant Repair of 8-Oxoguanine in Short DNA Bulges
AbstractThe presence of DNA damage can increase the likelihood of DNA replication errors and promote mutations. In particular, pauses of DNA...
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A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α–primase
The mechanism by which polymerase α–primase (polα–primase) synthesizes chimeric RNA-DNA primers of defined length and composition, necessary for...
