Search
Search Results
-
Targeted genome editing with a DNA-dependent DNA polymerase and exogenous DNA-containing templates
Reverse transcriptases, used in prime editing systems, exhibit lower fidelity, processivity and dNTP affinity than many DNA-dependent DNA...
-
Regulation of Human DNA Primase-Polymerase PrimPol
AbstractTransmission of genetic information depends on successful completion of DNA replication. Genomic DNA is subjected to damage on a daily basis....
-
Non-Catalytic Domains of DNA Polymerase λ: Influence on Enzyme Activity and Its Regulation
AbstractDNA polymerase λ (Polλ) belongs to the same structural X-family as DNA polymerase β, the main polymerase of base excision repair. The role of...
-
A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α–primase
The mechanism by which polymerase α–primase (polα–primase) synthesizes chimeric RNA-DNA primers of defined length and composition, necessary for...
-
An enhanced activity and thermostability of chimeric Bst DNA polymerase for isothermal amplification applications
AbstractLoop-mediated isothermal amplification (LAMP) is a widely used method for clinical diagnosis, customs quarantine, and disease prevention....
-
Influence of Nucleotide Context on Non-Specific Amplification of DNA with Bst exo– DNA Polymerase
AbstractIsothermal nucleic acids amplification that requires DNA polymerases with strand-displacement activity gained more attention in the last two...
-
Telomere C-Strand Fill-In Machinery: New Insights into the Human CST-DNA Polymerase Alpha-Primase Structures and Functions
Telomeres at the end of eukaryotic chromosomes are extended by a specialized set of enzymes and telomere-associated proteins, collectively termed...
-
Abundant and cosmopolitan lineage of cyanopodoviruses lacking a DNA polymerase gene
Cyanopodoviruses affect the mortality and population dynamics of the unicellular picocyanobacteria Prochlorococcus and Synechococcus , the dominant...
-
When push comes to shove - RNA polymerase and DNA-bound protein roadblocks
In recent years, transcriptional roadblocking has emerged as a crucial regulatory mechanism in gene expression, whereby other DNA-bound obstacles can...
-
Customized multiple sequence alignment as an effective strategy to improve performance of Taq DNA polymerase
AbstractEngineering Taq DNA polymerase ( Taq Pol) for improved activity, stability and sensitivity was critical for its wide applications. Multiple...
-
Impeding DNA Polymerase β Activity by Oleic Acid to Inhibit Base Excision Repair and Induce Mitochondrial Dysfunction in Hepatic Cells
Free fatty acids (FFAs) hepatic accumulation and the resulting oxidative stress contribute to several chronic liver diseases including nonalcoholic...
-
Repurposing FDA approved drugs against monkeypox virus DNA dependent RNA polymerase: virtual screening, normal mode analysis and molecular dynamics simulation studies
Zoonotic monkeypox disease, caused by the double-stranded DNA monkeypox virus, has become a global concern. Due to the absence of a specific small...
-
Detection and characterization of constitutive replication origins defined by DNA polymerase epsilon
BackgroundDespite the process of DNA replication being mechanistically highly conserved, the location of origins of replication (ORI) may vary from...
-
Directed evolution and selection of biostable l-DNA aptamers with a mirror-image DNA polymerase
Mirror-image aptamers made from chirally inverted nucleic acids are nuclease-resistant and exceptionally biostable, opening up opportunities for...
-
In vivo inhibition of the Ostreid Herpesvirus-1 (OsHV-1) replication in juveniles of the Pacific oyster Crassostrea gigas by a specific RNAi targeting the viral DNA polymerase gene
The global industrial farming of the Pacific oyster ( Crassostrea gigas ) has faced significant losses in production and a major negative social impact...
-
DNA polymerase iota promotes EMT and metastasis of esophageal squamous cell carcinoma by interacting with USP7 to stabilize HIF-1α
Esophageal squamous cell carcinoma (ESCC) is one of the most lethal cancer types, with a low 5-year survival rate of ~20%. Our prior research has...
-
The P286R mutation of DNA polymerase ε activates cancer-cell-intrinsic immunity and suppresses endometrial tumorigenesis via the cGAS-STING pathway
Endometrial carcinoma (EC) is a prevalent gynecological tumor in women, and its treatment and prevention are significant global health concerns. The...
-
DNA polymerase beta connects tumorigenicity with the circadian clock in liver cancer through the epigenetic demethylation of Per1
The circadian-controlled DNA repair exhibits a strong diurnal rhythm. Disruption in circadian clock and DNA repair is closely linked with...
-
Molecular evolution steered structural adaptations in the DNA polymerase III α subunit of halophilic bacterium Salinibacter ruber
A significant portion of the earth has a salty environment, and the literature on bacterial survival mechanisms in salty environments is limited....
-
Inhibition of Monkeypox Virus DNA Polymerase Using Moringa oleifera Phytochemicals: Computational Studies of Drug-Likeness, Molecular Docking, Molecular Dynamics Simulation and Density Functional Theory
The emergence of zoonotic monkeypox (MPX) disease, caused by the double-stranded DNA monkeypox virus (MPXV), has become a global threat. Due to...
