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Developmental Toxicity Using the Rat Whole Embryo Culture
In vivo and in vitro experiments have been used to investigate the effect of drugs, chemical agents, growth factors, vitamins, etc., on embryonic...
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Rat Embryonic Stem Cell Transgenesis
The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool...
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Electroporation-Mediated CRISPR/Cas9 Genome Editing in Rat Zygotes
Genetic engineering in the rat has been revolutionized by the development of CRISPR-based genome editing tools. Conventional methods for inserting...
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CRISPR-based genome editing of a diurnal rodent, Nile grass rat (Arvicanthis niloticus)
BackgroundDiurnal and nocturnal mammals have evolved distinct pathways to optimize survival for their chronotype-specific lifestyles. Conventional...
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Characterization of homozygous Foxn1 mutations induced in rat embryos by different delivery forms of Cas9 nuclease
BackgroundThe Cas9 nuclease is delivered in the form of either Cas9 protein or mRNA along with CRISPR guide RNA (gRNA: dual-crRNA:tracrRNA or...
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Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence
Studying various animal models is important for comparative biology and to better understand evolutionary development. Furthermore, when aiming to...
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Generating human blastoids modeling blastocyst-stage embryos and implantation
Human early development sets the stage for embryonic and adult life but remains difficult to investigate. A solution came from the ability of stem...
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An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells
Pigs share anatomical and physiological traits with humans and can serve as a large-animal model for translational medicine. Bona fide porcine...
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Adult hair follicle stem cells differentiate into neuronal cells in explanted rat intestinal tissue
Hair follicle stem cells (HFSCs) are adult stem cells located in the outer root sheath of the follicle bulge with high neural plasticity, which...
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Alternative splicing of CARM1 regulated by LincGET-guided paraspeckles biases the first cell fate in mammalian early embryos
The heterogeneity of CARM1 controls first cell fate bias during early mouse development. However, how this heterogeneity is established is unknown....
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CRISMERE Chromosome Engineering in Mouse and Rat
CRISPR/Cas9 technology is a versatile tool for engineering biology that has dramatically transformed our ability to manipulate genomes. In this...
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Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
Mammalian embryos sequentially differentiate into trophectoderm and an inner cell mass, the latter of which differentiates into primitive endoderm...
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Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos
Base editors have substantial promise in basic research and as therapeutic agents for the correction of pathogenic mutations. The development of...
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Optochemical Control of Cell Contractility in Drosophila Embryos
Cell shape changes based on actomyosin contractility provide a driving force in tissue morphogenesis. The temporally and spatially coordinated...
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The proteomic analysis of bovine embryos developed in vivo or in vitro reveals the contribution of the maternal environment to early embryo
BackgroundDespite many improvements with in vitro culture systems, the quality and developmental ability of mammalian embryos produced in vitro are...
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Precise and scalable self-organization in mammalian pseudo-embryos
Gene expression is inherently noisy, posing a challenge to understanding how precise and reproducible patterns of gene expression emerge in mammals....
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Deriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos Using Physical Distancing and Signal Inhibition
Until recently, naïve pluripotent stem cell lines were not captured from human embryos because protocols were based upon those devised for murine...
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Live-Cell Superresolution Imaging of Retrograde Axonal Trafficking Using Pulse–Chase Labeling in Cultured Hippocampal Neurons
The entanglement of long axons found in cultured dissociated hippocampal neurons restricts the analysis of the machinery underlying directed axonal...
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Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat
Laboratory rats have been used in biomedical research for over 170 years. Recently, genome editing technology has facilitated the generation of...
