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Further culture-independent characterization of the lactic microbiota of Serro artisanal cheese
This study aimed to provide a further characterization of the lactic microbiota present in Minas artisanal cheese (MAC) from the Serro region by...
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Bacterial diversity of an acid mine drainage beside the **chú River (Mexico) accessed by culture-dependent and culture-independent approaches
**chú River is a Mexican river located in an environmental preservation area called Sierra Gorda Biosphere Reserve. Around it, there are tons of...
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Bacterial Diversity in a Sri Lankan Geothermal Spring Assessed by Culture-Dependent and Culture-Independent Approaches
Hot springs harbour diverse and interesting groups of microorganisms adapted to extreme conditions. However, due to limitations in the...
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Antimicrobial resistance in urinary pathogens and culture-independent detection of trimethoprim resistance in urine from patients with urinary tract infection
BackgroundAlthough urinary tract infections (UTIs) are extremely common, isolation of causative uropathogens is not always routinely performed, with...
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Culture-independent analysis of the bacterial community in Chinese fermented vegetables and genomic analysis of lactic acid bacteria
Six different fermented vegetables were collected from Zhejiang Province, China, to explore the associated bacterial community using a...
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Isolation and characterization of novel potassium-solubilizing purple nonsulfur bacteria from acidic paddy soils using culture-dependent and culture-independent techniques
The current research as aimed (i) to isolate and select the purple nonsulfur bacteria (PNSB) possessing the potassium-solubilizing ability from acid...
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A culture-independent approach, supervised machine learning, and the characterization of the microbial community composition of coastal areas across the Bay of Bengal and the Arabian Sea
BackgroundCoastal areas are subject to various anthropogenic and natural influences. In this study, we investigated and compared the characteristics...
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Culture-dependent and culture-independent characterization of bacterial community diversity in different types of sandy lands: the case of Minqin County, China
BackgroundMinqin is suffering from a serious desertification, whereas the knowledge about its bacterial community is limited. Herein, based on Nitrari...
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Agrobacterium-mediated in planta transformation of cut coleoptile: a new, simplified, and tissue culture-independent method to deliver the CRISPR/Cas9 system in rice
BackgroundAgrobacterium -mediated transformation and particle bombardment are the two common approaches for genome editing in plant species using...
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Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review
Undesirable microbial infiltration into the female bovine reproductive tracts, for example during calving or mating, is likely to disturb the...
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Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol
Mouse embryonic stem cells (mESCs) were first derived and cultured nearly 30 years ago and have been beneficial tools to create transgenic mice and...
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Microbial Community of an 11th Century Manuscript by Both Culture-Dependent and -Independent Approaches
AbstractIn this study, the microbial community ( Bacteria, Archaea, and Fungi ) of a historical manuscript was investigated by microscopic observation...
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Diverse key nitrogen cycling genes nifH, nirS and nosZ associated with Pichavaram mangrove rhizospheres as revealed by culture-dependent and culture-independent analyses
Mangroves are highly productive unique ecosystems harboring diverse unexplored microbial communities that play crucial roles in nutrient cycling as...
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Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms
Viroids are single-stranded circular RNA molecules that cause diseases in plants and do not encode any protein. Classical approaches for the...
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Diversity and composition of the North Sikkim hot spring mycobiome using a culture-independent method
Fungi are considered to be the most resilient and economically important microbial community that can easily survive and optimally grow under a wide...
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Breaking down barriers: comprehensive functional analysis of the Aspergillus niger chitin synthase repertoire
BackgroundMembers of the fungal kingdom are heterotrophic eukaryotes encased in a chitin containing cell wall. This polymer is vital for cell wall...
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Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia
The hot spring Vranjska Banja is the hottest spring on the Balkan Peninsula with a water temperature of 63–95 °C and a pH value of 7.1, in situ....
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Statistical and Computational Methods for Microbial Strain Analysis
Microbial strains are interpreted as a lineage derived from a recent ancestor that have not experienced “too many” recombination events and can be...
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Analysis of the bacterial diversity in Moroccan Jben cheese using TTGE, DGGE, and 16S rRNA sequencing
This research investigated the physicochemical, microbiological, and bacterial diversity of Jben cheese, a popular artisanal variety in Morocco. The...
