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Characterization of Seed Mycobiota Using Culture-Dependent and Culture-Independent Approaches
Seed fungi are potentially important for their roles in seedling microbiome assembly and seedling health, but surveys of full seed fungal communities...
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Microbial Community of an 11th Century Manuscript by Both Culture-Dependent and -Independent Approaches
AbstractIn this study, the microbial community ( Bacteria, Archaea, and Fungi ) of a historical manuscript was investigated by microscopic observation...
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Isolation and characterization of novel potassium-solubilizing purple nonsulfur bacteria from acidic paddy soils using culture-dependent and culture-independent techniques
The current research as aimed (i) to isolate and select the purple nonsulfur bacteria (PNSB) possessing the potassium-solubilizing ability from acid...
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Bacterial Diversity in a Sri Lankan Geothermal Spring Assessed by Culture-Dependent and Culture-Independent Approaches
Hot springs harbour diverse and interesting groups of microorganisms adapted to extreme conditions. However, due to limitations in the...
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Bacterial diversity of an acid mine drainage beside the **chú River (Mexico) accessed by culture-dependent and culture-independent approaches
**chú River is a Mexican river located in an environmental preservation area called Sierra Gorda Biosphere Reserve. Around it, there are tons of...
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Culture-dependent screening of endospore-forming clostridia in infant feces
BackgroundOnly a few studies dealt with the occurrence of endospore-forming clostridia in the microbiota of infants without obvious health...
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Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater
BackgroundArsenic (As) and its species are major pollutants in ecological bodied including groundwater in Bangladesh rendering serious public health...
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Culture-dependent and culture-independent characterization of bacterial community diversity in different types of sandy lands: the case of Minqin County, China
BackgroundMinqin is suffering from a serious desertification, whereas the knowledge about its bacterial community is limited. Herein, based on Nitrari...
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Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol
Mouse embryonic stem cells (mESCs) were first derived and cultured nearly 30 years ago and have been beneficial tools to create transgenic mice and...
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Microbiological and Biomolecular Methods Applicable to the Isolation, Characterization and Identification of Microbial Sourdough Strains
The use of sourdough in high-quality fermentation processes is characterized by a microbiome composed of bacteria and yeasts cohabiting in a certain...
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Methods Used for Studying Human Microbiome
Human microbiome is a complex and dynamic component of the human system. Identifying the members of this vast community and elucidating their...
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Media matters: culture medium-dependent hypervariable phenotype of mesenchymal stromal cells
BackgroundDespite a long history of investigation and sustained efforts in clinical testing, the number of market authorisations for mesenchymal...
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Diverse key nitrogen cycling genes nifH, nirS and nosZ associated with Pichavaram mangrove rhizospheres as revealed by culture-dependent and culture-independent analyses
Mangroves are highly productive unique ecosystems harboring diverse unexplored microbial communities that play crucial roles in nutrient cycling as...
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Measuring frequency-dependent selection in culture
The frequency of a cultural trait can influence its tendency to be copied. We develop a maximum-likelihood method to measure such frequency-dependent...
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Overcoming BCR::ABL1 dependent and independent survival mechanisms in chronic myeloid leukaemia using a multi-kinase targeting approach
BackgroundDespite improved patient outcome using tyrosine kinase inhibitors (TKIs), chronic myeloid leukaemia (CML) patients require life-long...
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Bone morphogenetic protein 4 inhibits rat stem/progenitor Leydig cell development and regeneration via SMAD-dependent and SMAD-independent signaling
Bone morphogenetic protein 4 (BMP4) is an important member of the transforming growth factor-β superfamily. BMP4 is expressed in the Leydig cell...
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Promoting genotype-independent plant transformation by manipulating developmental regulatory genes and/or using nanoparticles
Key messageThis review summarizes the molecular basis and emerging applications of developmental regulatory genes and nanoparticles in plant...
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ACE2-dependent and -independent SARS-CoV-2 entries dictate viral replication and inflammatory response during infection
Excessive inflammation is the primary cause of mortality in patients with severe COVID-19, yet the underlying mechanisms remain poorly understood....
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Assessment of Placental Sodium-Independent Leucine Uptake and Transfer in Trophoblast Cells
The placenta maintains the balance between nutrition and growth control of the fetus through selective and regulated supply of macronutrients such as...
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Isolation and Identification of Growth-Promoting Bacteria from Plants Growing Under Abiotic Stresses
Identification and isolation of plant growth-promoting bacteria (PGPB) are critical steps toward understanding the role of these bacteria in stress...