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A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants
BackgroundVirus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies...
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A cytosol-tethered YHB variant of phytochrome B retains photomorphogenic signaling activity
The red and far-red light photoreceptor phytochrome B (phyB) transmits light signals following cytosol-to-nuclear translocation to regulate...
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Integrated Analyses of Metabolomic Profiling and Associated Gene Expression of Catharanthus roseus Seedling Reveal the Metabolic Alternations of Primary Metabolites and Flavonoids During the Apical Hook Opening Phase
In plants, apical hook opening is critical for the post-germination stage and subsequent photomorphogenesis, and this process is activated by a...
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Distinguishing among Pisum accessions using a hypervariable intron within Mendel’s green/yellow cotyledon gene
Distinguishing among commercial varieties or accessions in a germplasm repository presents considerable challenges when dealing with common crops or...
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Existence of giant mitochondria-containing sheet structures lacking cristae and matrix in the etiolated cotyledon of Arabidopsis thaliana
Mitochondria are essential organelles involved in the production and supply of energy in eukaryotic cells. Recently, the use of serial section...
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Variation of fatty acid composition in different tissues during Astragalus membranaceus seedling morphogenesis
Morphogenesis is a critical stage for the successful seedling establishment, which relies on energy provided by seed storage. Lipids are utilized as...
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Exogenous trehalose ameliorates methyl viologen induced oxidative stress through regulation of stomatal pore opening and glutathione metabolism in tomato seedlings
Oxidative burst is the common thread linking all abiotic and biotic stressors. So in this study non-reducing disaccharide trehalose (Tre) has been...
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Monitoring dissociation of chimerism through real-time PCR and scanning electron microscopy following in planta transformation of rough lemon (Citrus jambhiri Lush.)
Citrus spp. are recalcitrant to in vitro shoot regeneration and we report an improved in planta protocol for genetic transformation of rough lemon...
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Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitors
After germination, plantsPlants determine their morphogenesis, such as hypocotyl elongation and cotyledon opening, by responding to various... -
Somatic embryo induction and plantlet regeneration of Canna × generalis from immature zygotic embryo
Somatic embryogenesis is a unique method of in vitro regeneration, which can be used in plant reproduction, germplasm conservation, and...
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Light Perception and Transduction
Plants utilize light not only for photosynthesis but also as an environmental signal for many developmental processes. Plants are highly sensitive to... -
Actinidia seed-borne latent virus (ASbLV, Betaflexiviridae) is transmitted paternally and maternally at high rates
Actinidia seed-borne latent virus (ASbLV, Betaflexiviridae , genus Prunevirus) was detected at high frequency in healthy seedlings grown from lines of...
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Ca2+-Dependent Regulation of Proton Permeability of the Inner Membrane in the Mitochondria of Lupine Cotyledons
AbstractThe effect of exogenous Ca 2+ on respiration, generation of membrane potential, change in volume, and proton permeability of the inner...
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Phenotypic Study of Photomorphogenesis in Arabidopsis Seedlings
Light is one of the most important environmental factors, serving as the energy source of photosynthesis and a cue for plant developmental programs,... -
Overexpression of phosphoenol pyruvate carboxylase gene of Flaveria trinervia in transgenic mulberry (Morus spp.) leads to improved photosynthesis rate and tolerance to drought and salinity stresses
This study developed three transgenic mulberry lines containing heterologous phosphoenol pyruvate carboxylase gene of Flaveria trinervia through Agroba...
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How the seed coat affects the mother’s oviposition preference and larval performance in the bean beetle (Acanthoscelides obtectus, Coleoptera: Chrysomelidae, Bruchinae) in leguminous species
BackgroundThe host specificity and host range of the dry bean beetle, Acanthoscelides obtectus (Coleoptera: Chrysomelidae, Bruchinae), a seed...
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Arabidopsis COP1 guides stomatal response in guard cells through pH regulation
Plants rely on precise regulation of their stomatal pores to effectively carry out photosynthesis while managing water status. The Arabidopsis ...
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Deep learning-based detection of seedling development
BackgroundMonitoring the timing of seedling emergence and early development via high-throughput phenoty** with computer vision is a challenging...
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Genetic Markers and Biotechnology
Genetic diversity studies have immense importance as it provides information for selection of suitable genotype for hybridization. Cowpea is a... -
Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings
The preparation of biological samples, especially for live-cell microscopy, remains a major experimental challenge in the lab despite technological...