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High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis
Exploring how combinatorial mutations can be combined to optimize protein functions is important to guide protein engineering. Given the vast...
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Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations
Multiplexed genetic perturbations are critical for testing functional interactions among coding or non-coding genetic elements. Compared to...
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Role of Biotechnology and Combinatorial Chemistry Approaches in Molecular-Assisted Engineering of Plant Volatile Compounds
Plants produce a wide range of volatile compounds, which plays a crucial role in their growth, development, and interactions with the environment. In...
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Combinatorial metabolic engineering enables high yield production of α-arbutin from sucrose by biocatalysis
α-Arbutin has been widely used as a skin-whitening ingredient. Previously, we successfully produced α-arbutin via whole-cell biocatalysis and found...
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Phenylalanine ammonia-lyases: combining protein engineering and natural diversity
AbstractIn this study, rational design and saturation mutagenesis efforts for engineering phenylalanine ammonia-lyase from Petroselinum crispum ( Pc PA...
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From Genome Mining to Protein Engineering: A Structural Bioinformatics Route
This chapter outlines applications in genome mining, along with computational methods to predict protein structure and protein-ligand docking. It...
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Protein Engineering: Advances in Phage Display for Basic Science and Medical Research
AbstractFunctional Protein Engineering became the hallmark in biomolecule manipulation in the new millennium, building on and surpassing the...
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CRISPR/Cas9-based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii
AbstractKomagataella phaffii , a nonconventional yeast, is increasingly attractive to researchers owing to its posttranslational modification...
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Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules
The human gut microbiome modulates physiological functions and pathologies; however, a mechanistic understanding of microbe–host and microbe–microbe...
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Single-cell map** of combinatorial target antigens for CAR switches using logic gates
Identification of optimal target antigens that distinguish cancer cells from normal surrounding tissue cells remains a key challenge in chimeric...
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Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System
Yeast surface display has proven to be a powerful tool for the discovery of antibodies and other novel binding proteins and for engineering the...
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Site-wise Diversification of Combinatorial Libraries Using Insights from Structure-guided Stability Calculations
Many auspicious clinical and industrial accomplishments have improved the human condition by means of protein engineering. Despite these...
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Current challenges in designer cellulosome engineering
AbstractDesigner cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the...
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Soil microbiome engineering for sustainability in a changing environment
Recent advances in microbial ecology and synthetic biology have the potential to mitigate damage caused by anthropogenic activities that are...
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Thermostability enhancement and insight of L-asparaginase from Mycobacterium sp. via consensus-guided engineering
AbstractAcrylamide alleviation in food has represented as a critical issue due to its neurotoxic effect on human health. L-Asparaginase (ASNase, EC...
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Machine learning: an advancement in biochemical engineering
One of the most remarkable techniques recently introduced into the field of bioprocess engineering is machine learning. Bioprocess engineering has...
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Drug Design Methods to Regulate Protein–Protein Interactions
Inhibiting anomalous protein–protein interactions has led to the discovery of drugs (small molecules, precisely) that can inhibit such interactions...
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Plants against cancer: towards green Taxol production through pathway discovery and metabolic engineering
The diversity of plant natural products presents a rich resource for accelerating drug discovery and addressing pressing human health issues....
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Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation
Yeast surface display is a powerful directed evolution method for develo** and engineering protein molecules to attain desired properties. Here,...
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Opportunities and challenges in design and optimization of protein function
The field of protein design has made remarkable progress over the past decade. Historically, the low reliability of purely structure-based design...
