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Collagenase-Induced Osteoarthritis in Mice
The collagenase-induced experimental osteoarthritis model is in general applied in mice but can also be used in other small species. The model is...
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Synovium-Derived Mesenchymal Stem Cell-Based Scaffold-Free Fibrocartilage Engineering for Bone–Tendon Interface Healing in an Anterior Cruciate Ligament Reconstruction Model
Background:Current tendon and ligament reconstruction surgeries rely on scar tissue healing which differs from native bone-to-tendon interface (BTI)...
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Variability of BMP-2 content in DBM products derived from different long bone
Demineralized bone matrix (DBM) has been regarded as an ideal bone substitute as a native carrier of bone morphogenetic proteins (BMPs) and other...
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Isolation of Chondrons from Hyaline Cartilage
In native healthy hyaline cartilage, the chondrocytes are surrounded by a pericellular matrix that has a distinct composition and function compared...
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Insufficiency of collagenases in establishment of primary chondrocyte culture from cartilage of elderly patients receiving total joint replacement
Background Collagenases are frequently used in chondrocyte isolation from articular cartilage. However, the sufficiency of this enzyme in...
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Effect of Vasopressin on Collagenase Activity in Rat Papillary Renal Cells
AbstractTo find out whether matrix metalloproteinases, which comprise all classes of collagenases (including gelatinases), can be involved in the...
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Isolation of Human Adipose-Derived Stromal/Stem Cells from Lipoaspirates
Adipose tissue is an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative...
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Evaluation of non-invasive dsRNA delivery methods for the development of RNA interference in the Asian tiger mosquito Aedes albopictus
The Asian tiger mosquito Aedes albopictus is one of the most invasive species and an efficient vector of several pathogens. RNA interference (RNAi)...
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Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method
Adipose tissue provides a valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely...
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Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation
Adipose-derived stromal cells (ASC) are a promising alternative cell source to chondrocytes as well as to bone marrow-derived mesenchymal stromal...
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In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals
BackgroundHalophyte plant (HPs), a salt-resistant flora, has been reported to provide several health benefits, but the knowledge of its cosmeceutical...
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Ultrasound cavitation: a reliable non-enzymatic method for adipose-derived mesenchymal stem cell (ADSC) isolation
BackgroundAdipose tissue is known to serve as an abundant and readily accessible source of adipose-derived stem cells (ADSCs) as an alternative to...
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Establishment and characterization of a skeletal myoblast cell line of grass carp (Ctenopharyngodon idellus)
Skeletal muscle myoblastic cell lines can provide a valuable new in vitro model for the exploration of the mechanisms that control skeletal muscle...
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Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses
BackgroundSingle-cell RNA sequencing (scRNA-seq) is a powerful tool for studying complex biological systems, such as tumor heterogeneity and tissue...
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Extracts from the Liverwort Bazzania trilobata with Potential Dermo-cosmetic Properties
Bazzania trilobata (L.) Gray is a leafy liverwort from the family of Lepidoziaceae, well known for its antifungal properties. In this study, the 70%...
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Extracts from the Liverwort Bazzania trilobata with Potential Dermo-cosmetic Properties
Bazzania trilobata (L.) Gray is a leafy liverwort from the family of Lepidoziaceae, well known for its antifungal properties. In this study, the 70%...
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Interstitial collagenase MMP-1 and EMMPRIN in cell lines and in clinical specimens of cervical squamous cell carcinoma
BackgroundThe aim of this study was to elucidate the features of the expression of matrix metalloproteinases inducer—EMMPRIN (EMN) and matrix...
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Riboflavin-Induced Photocrosslinking of Highly Concentrated Collagen: Printing Accuracy, Degradation Time, and Cytocompatibility
The effect of riboflavin-induced collagen photocrosslinking on the scaffold printing accuracy, namely on the area of formed niches and filament...
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Mentha piperita powder enhances the biological response, growth performance, disease resistance, and survival of Oreochromis niloticus infected with Vibrio alginolyticus
Recently, medical plants have been widely used as replacements for antibiotics in disease treatment. Because of its multiple medicinal uses,...
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Channelrhodopsins for Cell-Type Specific Illumination of Cardiac Electrophysiology
Optogenetic approaches have evolved as potent means to investigate cardiac electrophysiology, with research ranging from the study of arrhythmia...