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Fibroblast-Epithelium Co-culture Methods Using Epithelial Organoids and Cell Line–Derived Spheroids
Fibroblasts are an integral cell type of mammary gland stroma, which plays crucial roles in development, homeostasis, and tumorigenesis of mammary...
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Medulloblastoma and high-grade glioma organoids for drug screening, lineage tracing, co-culture and in vivo assay
Medulloblastoma and high-grade glioma represent the most aggressive and frequent lethal solid tumors affecting individuals during pediatric age....
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The effects of co-culture on the expression of selected PKS genes in the lichenized fungus Xanthoparmelia taractica
Lichens produce diverse secondary metabolites. A diversity of these compounds is synthesized by fungal polyketide synthases (PKSs). In this study, we...
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Exploring the Cocktail Factor Approach to Generate Salivary Gland Progenitors through Co-Culture Techniques
Background:The derivation of salivary gland (SG) progenitors from pluripotent stem cells (PSCs) presents significant potential for developmental...
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Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture – interaction studies based on a multi-OMICs approach
In their natural habitats, microbes rarely exist in isolation; instead, they thrive in consortia, where various interactions occur. In this study, a...
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Differentiation of Fibroblasts to Adipocytes in 3D for a Co-culture with Mammary Organoids and Immunohistological Analysis
Mammary epithelial ducts, the main functional compartment of the mammary gland, are embedded in an adipocyte-rich stroma, which is essential for...
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Bioproduction of eriodictyol by Escherichia coli engineered co-culture
AbstractEriodictyol (ED) is a flavonoid in the flavanones subclass. It is abundantly present in a wide range of medicinal plants, citrus fruits, and...
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Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana
There is an expanding need to modify plant genomes to create new plant germplasm that advances both basic and applied plant research. Most current...
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Experimental Setup for Investigation of Acute Mitochondrial Oxygen Sensing in Primary Cells
The ability to sense and respond to acute changes in oxygen is essential for the viability of cells and organisms. To study molecular mechanisms of...
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Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene
Microbial communities involving dehalogenating bacteria assist in bioremediation of areas contaminated with halocarbons. To understand molecular...
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Fabrication Method of a High-Density Co-Culture Tumor–Stroma Platform to Study Cancer Progression
Cancer has now been established as one of the most common chronic diseases due to high mortality rate. The early stage of non-invasive tumors can now...
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A pumpless monolayer microfluidic device based on mesenchymal stem cell-conditioned medium promotes neonatal mouse in vitro spermatogenesis
BackgroundChildhood cancer treatment-induced gonadotoxicity causes permanent infertility/sub-infertility in nearly half of males. The current...
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Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids
Human primordial germ cells (hPGCs), the precursors of eggs and sperm, start their complex development shortly after specification and during their...
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BEHAV3D: a 3D live imaging platform for comprehensive analysis of engineered T cell behavior and tumor response
Modeling immuno-oncology by using patient-derived material and immune cell co-cultures can advance our understanding of immune cell tumor targeting...
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Co-cultures of Macrophages with Muscle Stem Cells with Fibroadipogenic Precursor Cells from Regenerating Skeletal Muscle
Adult muscle stem cells rebuild myofibers after damage. Although they are highly powerful to implement the adult myogenic program, they need...
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Generation and Culture of Primary Mouse Hepatocyte–Hepatic Stellate Cell Spheroids
In vitro models of liver fibrosis have evolved from mono-cultures of primary rodent hepatic stellate cells and stellate cell lines, to more complex...
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Chemotactic Interactions of Scenedesmus sp. and Azospirillum brasilense Investigated by Microfluidic Methods
The use of algae for industrial, biotechnological, and agricultural purposes is spreading globally. Scenedesmus species can play an essential role in...
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Co-culturing Human Intestinal Enteroid Monolayers with Innate Immune Cells
The coordinated interaction between the intestinal epithelium and immune cells is required to maintain proper barrier function and mucosal host...
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The cAMP receptor protein (CRP) enhances the competitive nature of Salmonella Typhimurium
The cAMP receptor protein (CRP) is a global regulatory protein. We evaluated the role of CRP in starvation physiology in Salmonella Typhimurium. The Δ
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A bioprocess perspective on the production of secondary metabolites by Streptomyces in submerged co-cultures
Filamentous microorganisms are potent sources of bioactive secondary metabolites, the molecules formed in response to complex environmental signals....