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Joint Distribution of Protein Concentration and Cell Volume Coupled by Feedback in Dilution
We consider a protein that negatively regulates the rate with which a cell grows. Since less growth means less protein dilution, this mechanism forms...
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Exploring operational boundaries for acoustic concentration of cell suspensions
AbstractThe development of a standardized, generic method for concentrating suspensions in continuous flow is challenging. In this study, we...
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Effects of Buffers Over the Cytosolic Calcium Concentration
In this chapter, we study the change in cellular calcium with different buffers. The study is carried over the advection–diffusion equation framed...
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Analysis of Cell Growth Kinetics in Suspension and Adherent Types of Cell Lines
Cell growth entails an increase in mass and physical size mostly influenced by physical, biological and chemical environments. As a result, each...
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Building Biomaterials to Mimic 3D Cell–Cell Junctions
Cell–cell interactions typically occur in a 3D context that is distinct from conventional 2D cell-substrate interactions in a Petri dish. Here, we...
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Probing Bacterial Cell Division and Cell Envelope Biogenesis with Live-Cell Fluorescence Microscopy
The development of advanced microscopy techniques has ushered in a new era of research as it helps understand biological processes on a deeper,...
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Practicalities of Cell Sorting
Cell sorting is a technique commonly used in academic and biotechnology laboratories in order to separate out cells or particles of interest from...
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HIV-1 Induced Cell-to-Cell Fusion or Syncytium Formation
HIV-1 cell-free infection has been thoroughly investigated; however, its relevance and importance in vitro are questionable. Cell-cell transmission...
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Cell Culture for Cell Penetrating Peptides
Animal cell culture is a vital part in basic as well as application oriented research initiatives in biological sciences. It is basically referred as...
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Cell Fractionation
Protein function is generally dependent on its subcellular localization. In gram-negative bacteria such as Escherichia coli, a protein can be...
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Cryopreservation of Cell Lines
Cryopreservation employs amalgamated applications of low temperatures in preserving structural composition along with functional integrity of animal...
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Upscaling of Clinical Grade Stem Cell Production: Upstream Processing (USP) and Downstream Processing (DSP) Operations of Cell Expansion, Harvesting, Detachment, Separation, Washing and Concentration Steps, and the Regulatory Requirements
This chapter has discussed very important issues in stem cell production for instance, the background of stem cell production, and upscaling of...
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Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation
Inducing the differentiation of specific cell type(s) synchronously and on-demand is a great experimental system to understand the sequential...
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Understanding Cell Differentiation Through Single-Cell Approaches: Conceptual Challenges of the Systemic Approach
The cells of a multicellular organism are derived from a single zygote and genetically almost identical. Yet, they are phenotypically very different....
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In Vitro Titan Cell Generation in Cryptococcus neoformans and Automated Cell Size Measurements
A special feature of the human fungal pathogen Cryptococcus neoformans is its morphological changes triggered by the interaction with the host....
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En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses
Imaging of the immunological synapse (IS) between dendritic cells (DCs) and T cells in suspension is hampered by suboptimal alignment of cell-cell...
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Cell Culture Laboratory
The first objective of cell culture laboratory is to maintain hygiene and keep it contamination-free. The design of a cell culture laboratory majorly...
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Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch
Cell viability of many cell types strongly relies on their ability to adjust to mechanical conditions and alterations. Cellular mechanisms for...
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Cell Cycle–Specific Protein Phosphatase 1 (PP1) Substrates Identification Using Genetically Modified Cell Lines
The identification of protein phosphatase 1 (PP1) holoenzyme substrates has proven to be a challenging task. PP1 can form different holoenzyme...
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Enhanced Methodologies for Investigating the Electrophysiological Characteristics of Endogenous Pannexin 1 Intercellular Cell–Cell Channels
Gap junctions, pivotal intercellular conduits, serve as communication channels between adjacent cells, playing a critical role in modulating membrane...