Search
Search Results
-
Undetectable off-target effects induced by FokI catalytic domain in mouse embryos
The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of...
-
Role of I182, R187, and K188 Amino Acid Residues in the Catalytic Domain of HIV-1 Integrase in the Processes of Reverse Transcription and Integration
AbstractStructural organization of HIV-1 integrase is based on a tetramer formed by two protein dimers. Within this tetramer, the catalytic domain of...
-
Autoprocessing and oxyanion loop reorganization upon GC373 and nirmatrelvir binding of monomeric SARS-CoV-2 main protease catalytic domain
The monomeric catalytic domain (residues 1–199) of SARS-CoV-2 main protease (MPro 1-199 ) fused to 25 amino acids of its flanking nsp4 region mediates...
-
Expression and Crystallization of HDAC6 Tandem Catalytic Domains
Histone deacetylase 6 (HDAC6) is an atypical lysine deacetylase with tandem catalytic domains and an ubiquitin-binding zinc finger domain. HDAC6 is...
-
An oligopeptide permease, OppABCD, requires an iron–sulfur cluster domain for functionality
Oligopeptide permease, OppABCD, belongs to the type I ABC transporter family. Its role is to import oligopeptides into bacteria for nutrient uptake...
-
Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach
The activity of deubiquitinases (DUBs) is tightly regulated in eukaryotes via various mechanisms. One of the regulatory strategies is...
-
Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase
AbstractCyclic β-1,2-glucan synthase (CGS) is a key enzyme in production of cyclic β-1,2-glucans (CβGs) which are involved in bacterial infection or...
-
Advances in the adenylation domain: discovery of diverse non-ribosomal peptides
AbstractNon-ribosomal peptide synthetases are mega-enzyme assembly lines that synthesize many clinically useful compounds. As a gatekeeper, they have...
-
Influence of NaCl and pH on lysostaphin catalytic activity, cell binding, and bacteriolytic activity
AbstractPeptidoglycan-degrading enzymes are a group of proteins intensively studied as novel antibacterials, with some of them having reached...
-
The catalytic triad of rice NARROW LEAF1 involves H234
NARROW LEAF1 ( NAL1 ) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is...
-
Investigating the effect of substrate binding on the catalytic activity of xylanase
AbstractXynAF1 from Aspergillus fumigatus Z5 is an efficient thermophilic xylanase belonging to glycoside hydrolase family 10 (GH10). The...
-
Non-Catalytic Domains of DNA Polymerase λ: Influence on Enzyme Activity and Its Regulation
AbstractDNA polymerase λ (Polλ) belongs to the same structural X-family as DNA polymerase β, the main polymerase of base excision repair. The role of...
-
Extremophilic Enzymes: Catalytic Features and Industrial Applications
Extremophilic microbes are those that are adapted to very harsh environmental conditions. Fungi constitute one of the important groups that by virtue...
-
Novel reaction systems for catalytic synthesis of structured phospholipids
AbstractPhospholipids are distinctive, adaptable molecules that are crucial to numerous biological systems. Additionally, their various architectures...
-
Catalytic-dependent and -independent roles of TET3 in the regulation of specific genetic programs during neuroectoderm specification
The ten-eleven-translocation family of proteins (TET1/2/3) are epigenetic regulators of gene expression. They regulate genes by promoting DNA...
-
Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation
Using Sanger sequencing and high-throughput genome sequencing of DNA cleavage reactions, we find that the Streptococcus pyogenes SpCas9 complex...
-
Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A)
N -Glycosylation is a common post-translational modification, and the number of GlcNAc branches in N -glycans impacts glycoprotein functions. N -Acetylgl...
-
Catalytic and Electrocatalytic Mechanisms of Cytochromes P450 in the Development of Biosensors and Bioreactors
AbstractCytochromes P450 are a unique family of enzymes found in all Kingdoms of living organisms (animals, bacteria, plants, fungi, and archaea),...
-
Measuring Protein Tyrosine Phosphatase Activity Dependent on SH2 Domain-Mediated Regulation
Src-homology-2 (SH2) domains bind selectively to phosphotyrosine (pTyr) residues located in target binding proteins; therefore, they are key elements...
-
AmyJ33, a truncated amylase with improved catalytic properties
Biochemical and kinetic properties are of special interest for the specific applications of α-amylases in industrial sectors such as textile...
