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Calcium Imaging of Non-adherent Cells
Live-cell imaging can reveal dynamic and multimodal cell signaling by monitoring calcium flux. Spatiotemporal changes in Ca2+ concentrations... -
Calcium imaging: a technique to monitor calcium dynamics in biological systems
Calcium ion (Ca 2+ ) is a multifaceted signaling molecule that acts as an important second messenger. During the course of evolution, plants and...
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Live-Cell Imaging of Peroxisomal Calcium Levels and Dynamics
Calcium (Ca2+) is an intracellular messenger that plays an essential role in a variety of cellular processes ranging from early embryonic events to... -
Detection of Male Olfactory Information by Female Goats: A Calcium Imaging Approach
In mammals, reproductive function can be modulated by sociosexual factors. In small ruminants such as goats, phenomena such as puberty acceleration... -
Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normotensive, Wistar Kyoto Rats and Spontaneously Hypertensive Rats
The spontaneously hypertensive rat (SHR) is a model widely used to investigate the causal mechanisms of essential hypertension. The enhanced... -
Detecting and correcting false transients in calcium imaging
Population recordings of calcium activity are a major source of insight into neural function. Large datasets require automated processing, but this...
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Reinforcing neuron extraction and spike inference in calcium imaging using deep self-supervised denoising
Calcium imaging has transformed neuroscience research by providing a methodology for monitoring the activity of neural circuits with single-cell...
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FIOLA: an accelerated pipeline for fluorescence imaging online analysis
Optical microscopy methods such as calcium and voltage imaging enable fast activity readout of large neuronal populations using light. However, the...
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Rapid detection of neurons in widefield calcium imaging datasets after training with synthetic data
Widefield microscopy can provide optical access to multi-millimeter fields of view and thousands of neurons in mammalian brains at video rate....
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Calcium Live Imaging at Multi-Scales from Cellular to Organ Level in Arabidopsis thaliana
Plants must adapt to environmental constraints. For this, they are able to perceive several types of stress in isolation or in combination manner. At... -
Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips
The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in...
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Calcium imaging in the NIR
The near-infrared calcium sensor iGECI shows promise for imaging neuronal activity in vivo.
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System-level time computation and representation in the suprachiasmatic nucleus revealed by large-scale calcium imaging and machine learning
The suprachiasmatic nucleus (SCN) is the mammalian central circadian pacemaker with heterogeneous neurons acting in concert while each neuron harbors...
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Intracellular calcium links milk stasis to lysosome-dependent cell death during early mammary gland involution
Involution of the mammary gland after lactation is a dramatic example of coordinated cell death. Weaning causes distension of the alveolar structures...
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Rasal1 regulates calcium dependent neuronal maturation by modifying microtubule dynamics
BackgroundRasal1 is a Ras GTPase-activating protein which contains C2 domains necessary for dynamic membrane association following intracellular...
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Engineering of NEMO as calcium indicators with large dynamics and high sensitivity
Genetically encoded calcium indicators (GECIs) are indispensable tools for real-time monitoring of intracellular calcium signals and cellular...
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Monitoring Calcium Fluxes and Lysosome Exocytosis During Pyroptosis
Inflammasome-mediated activation of inflammatory caspases (caspase-1, caspase-4, caspase-5, caspase-11) initiates a cascade of cellular events that... -
Motility Assay to Probe the Calcium Sensitivity of Myosin and Regulated Thin Filaments
Calcium-dependent activation of the thin filament mediated by the troponin-tropomyosin complex is key in the regulation of actin-myosin based muscle... -
Inhibition of Plasma Membrane Calcium Pump Influences Intracellular Calcium Signaling Pathways in Breast Cancer
The plasma membrane calcium pump (PMCA) is an important transporter that maintains intracellular calcium concentration ([Ca 2+ ] i ). It allows the...