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  1. Calcium Imaging of Non-adherent Cells

    Live-cell imaging can reveal dynamic and multimodal cell signaling by monitoring calcium flux. Spatiotemporal changes in Ca2+ concentrations...
    Lydia J. Bye, Rocio K. Finol-Urdaneta, David J. Adams in Cell Viability Assays
    Protocol 2023
  2. Calcium imaging: a technique to monitor calcium dynamics in biological systems

    Calcium ion (Ca 2+ ) is a multifaceted signaling molecule that acts as an important second messenger. During the course of evolution, plants and...

    Soma Ghosh, Monika Dahiya, ... Girdhar K. Pandey in Physiology and Molecular Biology of Plants
    Article 30 December 2023
  3. Live-Cell Imaging of Peroxisomal Calcium Levels and Dynamics

    Calcium (Ca2+) is an intracellular messenger that plays an essential role in a variety of cellular processes ranging from early embryonic events to...
    Yelena Sargsyan, Sven Thoms in Peroxisomes
    Protocol 2023
  4. Detection of Male Olfactory Information by Female Goats: A Calcium Imaging Approach

    In mammals, reproductive function can be modulated by sociosexual factors. In small ruminants such as goats, phenomena such as puberty acceleration...
    Maxime Meunier, Anne-Charlotte Trouillet, ... Pablo Chamero in Chemical Signals in Vertebrates 15
    Conference paper 2023
  5. Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normotensive, Wistar Kyoto Rats and Spontaneously Hypertensive Rats

    The spontaneously hypertensive rat (SHR) is a model widely used to investigate the causal mechanisms of essential hypertension. The enhanced...
    Tzitzitlini Alejandre-Garcƭa, Pedro Segura-Chama, ... Arturo HernƔndez-Cruz in Chromaffin Cells
    Protocol 2023
  6. New miniscope for optogenetics and calcium imaging

    Ioannis Bakoyiannis in Lab Animal
    Article 23 September 2022
  7. Detecting and correcting false transients in calcium imaging

    Population recordings of calcium activity are a major source of insight into neural function. Large datasets require automated processing, but this...

    Jeffrey L. Gauthier, Sue Ann Koay, ... Adam S. Charles in Nature Methods
    Article 28 March 2022
  8. Reinforcing neuron extraction and spike inference in calcium imaging using deep self-supervised denoising

    Calcium imaging has transformed neuroscience research by providing a methodology for monitoring the activity of neural circuits with single-cell...

    **nyang Li, Guoxun Zhang, ... Qionghai Dai in Nature Methods
    Article 16 August 2021
  9. FIOLA: an accelerated pipeline for fluorescence imaging online analysis

    Optical microscopy methods such as calcium and voltage imaging enable fast activity readout of large neuronal populations using light. However, the...

    Changjia Cai, Cynthia Dong, ... Andrea Giovannucci in Nature Methods
    Article 07 September 2023
  10. Rapid detection of neurons in widefield calcium imaging datasets after training with synthetic data

    Widefield microscopy can provide optical access to multi-millimeter fields of view and thousands of neurons in mammalian brains at video rate....

    Yuanlong Zhang, Guoxun Zhang, ... Qionghai Dai in Nature Methods
    Article Open access 01 April 2023
  11. Calcium Live Imaging at Multi-Scales from Cellular to Organ Level in Arabidopsis thaliana

    Plants must adapt to environmental constraints. For this, they are able to perceive several types of stress in isolation or in combination manner. At...
    Carine Alcon, Tou Cheu **ong in Plant Abiotic Stress Signaling
    Protocol 2023
  12. Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips

    The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in...

    Pierre-Ewen Lecoq, ChloƩ Dupuis, ... Jean-Luc Aider in npj Microgravity
    Article Open access 06 February 2024
  13. Calcium imaging in the NIR

    The near-infrared calcium sensor iGECI shows promise for imaging neuronal activity in vivo.

    Nina Vogt in Nature Methods
    Article 06 January 2021
  14. System-level time computation and representation in the suprachiasmatic nucleus revealed by large-scale calcium imaging and machine learning

    The suprachiasmatic nucleus (SCN) is the mammalian central circadian pacemaker with heterogeneous neurons acting in concert while each neuron harbors...

    Zichen Wang, **g Yu, ... He** Cheng in Cell Research
    Article Open access 11 April 2024
  15. Intracellular calcium links milk stasis to lysosome-dependent cell death during early mammary gland involution

    Involution of the mammary gland after lactation is a dramatic example of coordinated cell death. Weaning causes distension of the alveolar structures...

    Jaekwang Jeong, Jongwon Lee, ... John Wysolmerski in Cellular and Molecular Life Sciences
    Article Open access 12 January 2024
  16. Rasal1 regulates calcium dependent neuronal maturation by modifying microtubule dynamics

    Background

    Rasal1 is a Ras GTPase-activating protein which contains C2 domains necessary for dynamic membrane association following intracellular...

    M. H. S. Deurloo, S. Eide, ... Z.-P. Feng in Cell & Bioscience
    Article Open access 21 January 2024
  17. Engineering of NEMO as calcium indicators with large dynamics and high sensitivity

    Genetically encoded calcium indicators (GECIs) are indispensable tools for real-time monitoring of intracellular calcium signals and cellular...

    Jia Li, Ziwei Shang, ... Youjun Wang in Nature Methods
    Article Open access 20 April 2023
  18. Monitoring Calcium Fluxes and Lysosome Exocytosis During Pyroptosis

    Inflammasome-mediated activation of inflammatory caspases (caspase-1, caspase-4, caspase-5, caspase-11) initiates a cascade of cellular events that...
    Wendy P. Loomis, Tessa Bergsbaken in Pyroptosis
    Protocol 2023
  19. Motility Assay to Probe the Calcium Sensitivity of Myosin and Regulated Thin Filaments

    Calcium-dependent activation of the thin filament mediated by the troponin-tropomyosin complex is key in the regulation of actin-myosin based muscle...
    Chao Liu, Kathleen M. Ruppel, James A. Spudich in Familial Cardiomyopathies
    Protocol 2024
  20. Inhibition of Plasma Membrane Calcium Pump Influences Intracellular Calcium Signaling Pathways in Breast Cancer

    The plasma membrane calcium pump (PMCA) is an important transporter that maintains intracellular calcium concentration ([Ca 2+ ] i ). It allows the...

    Armagan Caner, Muge Gulcihan Onal in Cell Biochemistry and Biophysics
    Article 05 September 2022
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