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Exploration of three Dyadobacter fermentans enzymes uncovers molecular activity determinants in CE15
AbstractGlucuronoyl esterases (GEs) are serine-type hydrolase enzymes belonging to carbohydrate esterase family 15 (CE15), and they play a central...
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Transesterification with CE15 glucuronoyl esterase from Cerrena unicolor reveals substrate preferences
PurposeGlucuronoyl esterases (GE, family CE15) catalyse the cleavage of ester linkages in lignin-carbohydrate complexes (LCCs), and this study...
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Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling
Activity-based protein profiling (ABPP) has emerged as a versatile biochemical method for studying enzyme activity under various physiological...
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New insights to diversity and enzyme–substrate interactions of fungal glucuronoyl esterases
AbstractGlucuronoyl esterases (GEs) (EC 3.1.1.117) catalyze the cleavage of ester-linked lignin-carbohydrate complexes that has high impact on the...
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Enzyme kinetics of fungal glucuronoyl esterases on natural lignin-carbohydrate complexes
Glucuronoyl esterases (CE15 family) enable targeted cleavage of ester linkages in lignin-carbohydrate complexes (LCCs), particularly those linking...
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“Integrative genomic analysis of the bioprospection of regulators and accessory enzymes associated with cellulose degradation in a filamentous fungus (Trichoderma harzianum)”
BackgroundUnveiling fungal genome structure and function reveals the potential biotechnological use of fungi. Trichoderma harzianum is a powerful...
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Integrative analysis of genome and transcriptome reveal the genetic basis of high temperature tolerance in pleurotus giganteus (Berk. Karun & Hyde)
BackgroundPleurotus giganteus is a commonly cultivated mushroom with notable high temperature resistance, making it significant for the growth of...
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Comparative genomic analysis of Planctomycetota potential for polysaccharide degradation identifies biotechnologically relevant microbes
BackgroundMembers of the Planctomycetota phylum harbour an outstanding potential for carbohydrate degradation given the abundance and diversity of...
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Cellulase Hyper-Producing Fungus Penicillium janthinellum NCIM 1366 Elaborates a Wider Array of Proteins Involved in Transport and Secretion, Potentially Enabling a Diverse Substrate Range
The efficient breakdown of lignocellulose requires the concerted activity of multiple enzymes. Previous studies on Penicillium janthinellum NCIM 1366...
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A genomic view of the microbiome of coral reef demosponges
Sponges underpin the productivity of coral reefs, yet few of their microbial symbionts have been functionally characterised. Here we present an...
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Genome sequencing of Inonotus obliquus reveals insights into candidate genes involved in secondary metabolite biosynthesis
BackgroundInonotus obliquus is an important edible and medicinal mushroom that was shown to have many pharmacological activities in preclinical...
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A comparative study of microbial community and functions of type 2 diabetes mellitus patients with obesity and healthy people
The gut microbiota is crucial in the pathogenesis of type 2 diabetes mellitus (T2DM). However, the metabolism of T2DM patients is not...
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Ecological Genomics and Evolution of Trichoderma reesei
The filamentous fungus Trichoderma reesei (Hypocreales, Ascomycota) is an efficient industrial cell factory for the production of cellulolytic...
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Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives
Efficient saccharification of lignocellulosic biomass requires concerted development of a pretreatment method, an enzyme cocktail and an enzymatic...
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Genome sequencing and evolutionary analysis of a new endophytic Trichoderma species isolated from orchid roots with reduced repertoire of protein-coding genes
The cosmopolitan fungal genus Trichoderma contains species commonly found in other fungi, on plant litter, or living plants, showing parasitic,...
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Coconut rhinoceros beetle digestive symbiosis with potential plant cell wall degrading microbes
Coconut rhinoceros beetle (CRB, Oryctes rhinoceros ) is an invasive palm pest whose larvae eat wood, yet lack the necessary digestive enzymes. This...
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Transcriptional profile of oil palm pathogen, Ganoderma boninense, reveals activation of lignin degradation machinery and possible evasion of host immune response
BackgroundThe white-rot fungi in the genus Ganoderma interact with both living and dead angiosperm tree hosts. Two Ganoderma species, a North...
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Industrially Important Genes from Trichoderma
Trichoderma as a cosmopolitan genus with industrial, agricultural importance occupies a diversity of habitats. The notoriety of Trichoderma species...
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Expression and characterization of two glucuronoyl esterases from Thielavia terrestris and their application in enzymatic hydrolysis of corn bran
The thermophilic fungus Thielavia terrestris when cultured on cellulose produces a cocktail of thermal hydrolases with potential application in...
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A mini review of xylanolytic enzymes with regards to their synergistic interactions during hetero-xylan degradation
This review examines the recent models describing the mode of action of various xylanolytic enzymes and how these enzymes can be applied...
