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DNA Methylation Analysis Using Bisulfite Pyrosequencing
Pyrosequencing is a DNA sequencing-by-synthesis technique that can quantitatively detect single-nucleotide polymorphisms (SNPs). With pyrosequencing,...
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Optimized bisulfite sequencing analysis reveals the lack of 5-methylcytosine in mammalian mitochondrial DNA
BackgroundDNA methylation is one of the best characterized epigenetic modifications in the mammalian nuclear genome and is known to play a...
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DNA Methylation Analysis of Cnr1 Gene Promoter
DNA methylation pattern could be considered a biomarker to be exploited for the study and management of several human diseases. In this chapter,...
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Oxidative Bisulfite Sequencing: An Experimental and Computational Protocol
Bisulfite sequencing (BS-seq) remains the gold standard technique to quantitively map DNA methylation at a single-base resolution. However, BS-seq...
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Systemic interindividual DNA methylation variants in cattle share major hallmarks with those in humans
BackgroundWe recently identified ~ 10,000 correlated regions of systemic interindividual epigenetic variation (CoRSIVs) in the human genome. These...
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Sperm DNA methylation alterations from cannabis extract exposure are evident in offspring
BackgroundCannabis legalization is expanding and men are the predominant users. We have limited knowledge about how cannabis impacts sperm and...
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The DNA methylation status of the serotonin metabolic pathway associated with reproductive inactivation induced by long-light exposure in Magang geese
BackgroundDomestic geese are seasonal breeders and have the lowest reproductive capacity among all poultry species. Magang geese is a topical...
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Use of Advanced Molecular Techniques for Human Body Fluids Detection
Forensic body fluid analysis is being modernized by the application of advanced molecular techniques for human body fluids identification. Detection...
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Identification of cell type-specific methylation signals in bulk whole genome bisulfite sequencing data
BackgroundThe traditional approach to studying the epigenetic mechanism CpG methylation in tissue samples is to identify regions of concordant...
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MFSD4A inhibits the malignant progression of nasopharyngeal carcinoma by targeting EPHA2
DNA Methylation can lead to abnormal gene expression. In the present study, we investigated whether the expression of methylated MFSD4A (major...
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Does epigenetic markers of HLA gene show association with coronary artery disease in Indian subjects?
BackgroundDNA methylation, one of the most stable forms of epigenetic modification is associated with the development and progression of coronary...
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Genetic variation in the Y chromosome and sex-biased DNA methylation in somatic cells in the mouse
Several lines of evidence suggest that the presence of the Y chromosome influences DNA methylation of autosomal loci. To better understand the impact...
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Genome-wide DNA methylation analysis in schizophrenia with tardive dyskinesia: a preliminary study
BackgroundTardive dyskinesia (TD) develops in 20–30% of schizophrenia patients and up to 50% in patients > 50 years old. DNA methylation may play an...
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Genetic Testing in Cervical Cancer
Cervical cancer, a prevalent disease in develo** countries, is responsible for a huge number of deaths due to gynecological cancers. Human...
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Development and performance evaluation of TaqMan real-time fluorescence quantitative methylation specific PCR for detecting methylation level of PER2
BackgroundPER2 gene methylation is closely related to the occurrence and progress of some cancers, but there is no method to quantitatively detect...
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Imprinted DNA methylation of the H19 ICR is established and maintained in vivo in the absence of Kaiso
BackgroundPaternal allele-specific DNA methylation of the imprinting control region ( H19 ICR) controls genomic imprinting at the Igf2/H19 locus. We...
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Epigenetic clock in the aorta and age-related endothelial dysfunction in mice
While epigenetic age (EA) of mouse blood can be determined using DNA methylation analysis at three CpG sites in the Prima1 , Hsf4 and Kcns1 genes it...
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Laboratory methods to decipher epigenetic signatures: a comparative review
Epigenetics refers to nucleotide sequence-independent events, and heritable changes, including DNA methylation and histone modification (as the two...
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Introduction to Epigenetics
Epigenetics is the study of inherited variations in gene expression that do not involve changes to DNA sequences. Over 200 unique cell types in a...
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Epigenetics and DNA Methylation
Epigenetics is a wide term representing molecular processes leading to modification of gene expression without bringing any DNA base sequence...