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Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets
In vitro biopanning platforms using synthetic phage display antibody libraries have enabled the identification of antibodies against antigens that...
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Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum
Deep sequence-coupled biopanning (DSCB) is a powerful tool that couples affinity selection of a bacteriophage MS2 virus-like particle peptide display...
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Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting
Yeast surface display (YSD) emerged as a prominent screening methodology for the isolation of monoclonal antibodies (mAbs) against various antigens....
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Streptavidin-Coated Solid-Phase Extraction (SPE) Tips for Antibody Phage Display Biopanning
Phage display is a technique that allows the presentation of unique proteins on the surface of bacteriophages. The phage particles are usually...
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Antibody Isolation from Human Synthetic Libraries of Single-Chain Antibodies and Analysis Using NGS
Antibody libraries came into existence 30 years ago when the accumulating sequence data of immunoglobulin genes and the advent of PCR technology made...
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Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases
Phage display (PD) is a powerful method and has been extensively used to generate monoclonal antibodies and identify epitopes, mimotopes, and protein...
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Magnetic Nanoparticle-Based Semi-automated Panning for High-Throughput Antibody Selection
Bio-panning is a common process involved in recombinant antibody selection against defined targets. The biopanning process aims to isolate specific...
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Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display
Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells. This includes typical ECM structures such as collagen and...
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Generation of a novel antibody against BxPrx, a diagnostic marker of pine wilt disease
BackgroundBursaphelenchus xylophilus is a pathogenic nematode that causes pine wilt disease (PWD). To prevent the rapid spread of this pathogen,...
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HSP60 mimetic peptides from Mycobacterium leprae as new antigens for immunodiagnosis of Leprosy
The early diagnosis of leprosy serves as an important tool to reduce the incidence of this disease in the world. Phage display (PD) technology can be...
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Selection of Affibody Affinity Proteins from Phagemid Libraries
Herein, we describe a general protocol for the selection of target-binding affinity protein molecules from a phagemid-encoded library. The protocol...
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Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library
ObjectivesOvarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a...
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CD47Binder: Identify CD47 Binding Peptides by Combining Next-Generation Phage Display Data and Multiple Peptide Descriptors
CD47/SIRPα pathway is a new breakthrough in the field of tumor immunity after PD-1/PD-L1. While current monoclonal antibody therapies targeting...
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TUPDB: Target-Unrelated Peptide Data Bank
The isolation of target-unrelated peptides (TUPs) through biopanning remains as a major problem of phage display selection experiments. These TUPs do...
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Map** Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display
Peptide phage display has historically been used to epitope map monoclonal antibodies. More recently, by coupling this method with next-generation...
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Screening and Identification of a Specific Binding Peptide to Ovarian Cancer Cells from a Phage-Displayed Peptide Library
To select specific binding peptides for imaging and detection of human ovarian cancer. The phage 12-mer peptide library was used to select specific...
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Epivolve: A Protocol for Site-Directed Antibodies
Researchers can often successfully generate antibodies to predicted epitopes. Especially when the epitopes are on the surface of a protein or in a...
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Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites
Protein engineering has brought advances to industrial processes, biomaterials, nanotechnology, biosensors, and biomedical applications. This chapter...
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Application of Phage-Displayed Peptides in Tumor Imaging Diagnosis and Targeting Therapy
Phage display is an effective and powerful technique that provides a route to discovery unique peptides targeting to tumor cells. Specifically...
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Two-dimensional high-throughput on-cell screening of immunoglobulins against broad antigen repertoires
Identifying high-affinity antibodies in human serum is challenging due to extremely low number of circulating B cells specific to the desired...
