Search
Search Results
-
Recombinant T7 RNA polymerase production using ClearColi BL21(DE3) and animal-free media for in vitro transcription
AbstractIn vitro transcription (IVT) using T7 RNA polymerase (RNAP) is integral to RNA research, yet producing this enzyme in E. coli presents...
-
Effects of Bifidobacterium BL21 and Lacticaseibacillus LRa05 on gut microbiota in type 2 diabetes mellitus mice
Gut dysbiosis causes damage to the intestinal barrier and is associated with type 2 diabetes mellitus (T2DM). We tested the potential protective...
-
Tn5 Transposon-based Mutagenesis for Engineering Phage-resistant Strains of Escherichia coli BL21 (DE3)
Escherichia coli is a preferred strain for recombinant protein production, however, it is often plagued by phage infection during experimental...
-
Reduction in Serum Concentrations of Uremic Toxins Driven by Bifidobacterium Longum Subsp. Longum BL21 is Associated with Gut Microbiota Changes in a Rat Model of Chronic Kidney Disease
Gut microbiota dysbiosis and consequent impairment of gut barrier function, culminating in elevated levels of uremic toxins, are prevalent in chronic...
-
Expression of soluble moloney murine leukemia virus-reverse transcriptase in Escherichia coli BL21 star (DE3) using autoinduction system
Autoinduction systems in Escherichia coli can control the production of proteins without the addition of a particular inducer. In the present study,...
-
Expression of BSN314 lysozyme genes in Escherichia coli BL21: a study to demonstrate microbicidal and disintegarting potential of the cloned lysozyme
This study is an extension of our previous studies in which the lysozyme was isolated and purified from Bacillus subtilis BSN314 (Naveed et al.,...
-
Cloning and expression of staphylokinase-streptokinase recombinant protein in E. coli BL21(DE3)
Streptokinase (SK) is one of the essential fibrinolytic agents and is commonly employed to treat myocardial infarction or heart attack. Despite its...
-
Achieving high titer and yield in the bioconversion of l-threonine to 2-hydroxybutyric acid with Escherichia coli BL21
The study investigated the enhanced production of 2-hydroxybutyric acid (2-HBA) from threonine using a two-step whole-cell bioconversion by...
-
Separate expression and co-expression of MTSase and MTHase from Arthrobacter ramosus S34 in Escherichia coli BL21(DE3)
Maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose hydrolase (MTHase) are used to produce trehalose, a disaccharide of interest to...
-
Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity
Gene expression toxicity is an important biological phenomenon and a major bottleneck in biotechnology. Escherichia coli BL21(DE3) is the most...
-
Efficient anaerobic consumption of D-xylose by E. coli BL21(DE3) via xylR adaptive mutation
BackgroundMicroorganisms can prioritize the uptake of different sugars depending on their metabolic needs and preferences. When both D-glucose and...
-
Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N‑acetyl‑d‑neuraminic acid
N ‑Acetyl‑ d ‑neuraminic acid (Neu5Ac) is the crucial compound for the chemical synthesis of antiflu medicine Zanamivir. Chemoenzymatic synthesis of...
-
Enhancing the production of γ-aminobutyric acid in
Escherichia coli BL21 by engineering the enzymes of the regeneration pathway of the coenzyme factor pyridoxal 5’-phosphateThe compound γ-aminobutyric acid (GABA) was widely used in various fields. To enhance the production of GABA in Escherichia coli BL21(DE3), the...
-
Improvement in extracellular secretion of recombinant l-asparaginase II by Escherichia coli BL21 (DE3) using glycine and n-dodecane
l -asparaginase II (ASNase) is the biopharmaceutical of choice for the treatment of acute lymphoblastic leukaemia. In this study, E. coli BL21 (DE3)...
-
The Improved Expression and Stability of Zearalenone Lactonohydrolase from Escherichia coli BL21 (DE3)
AbstractZearalenone (ZEN) is an estrogenic mycotoxin produced by Fusarium fungi which is commonly found in cereal crops. Because of its estrogen...
-
Purification of Recombinant N-terminal Histidine-Tagged Arabidopsis thaliana Phosphoglycolate Phosphatase 1, Glycolate Oxidase 1 and 2, and Hydroxypyruvate Reductase 1
To measure the kinetic properties of photorespiratory enzymes, it is necessary to work with purified proteins. Protocols to purify photorespiratory...
-
Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3)
AbstractWith the goal of expanding the diversity of tools available for controlling gene expression in cyanobacteria, the T7-RNA polymerase gene...
-
High-level production of γ-cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21 (DE3): culture medium optimization, enzymatic properties characterization, and product specificity analysis
Purposeγ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is...
-
Probiotics Influence Gut Microbiota and Tumor Immune Microenvironment to Enhance Anti-Tumor Efficacy of Doxorubicin
Probiotics have been reported to influence the gut microbiota and immune system in various diseases. Now, the potential impacts of probiotics on...
-
Enhancement of acid tolerance of Escherichia coli by introduction of molecule chaperone CbpA from extremophile
Molecular chaperone CbpA from extreme acidophile Acidithiobacillus caldus was applied to improve acid tolerance of Escherichia coli via CRISPR/Cas9....
