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DG-Affinity: predicting antigen–antibody affinity with language models from sequences
BackgroundAntibody-mediated immune responses play a crucial role in the immune defense of human body. The evolution of bioengineering has led the...
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Extract antibody and antigen names from biomedical literature
BackgroundThe roles of antibody and antigen are indispensable in targeted diagnosis, therapy, and biomedical discovery. On top of that, massive...
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Attogram-level light-induced antigen-antibody binding confined in microflow
The analysis of trace amounts of proteins based on immunoassays and other methods is essential for the early diagnosis of various diseases such as...
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Development of a Humanized Antibody 5D3Hu against the PRAME Tumor Antigen
AbstractThe PRAME antigen, which is a significant target for monoclonal antibodies, is a tumor-specific marker that is active at all stages of tumor...
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Honing-in antigen-specific cells during antibody discovery: a user-friendly process to mine a deeper repertoire
Immunization based antibody discovery is plagued by the paucity of antigen-specific B cells. Identifying these cells is akin to finding needle in a...
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The Allosteric Effect in Antibody-Antigen Recognition
We studied the molecular details of the recognition of antigens by the variable domain of their cognate antibodies in as well as those elicited by...
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A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen
AbstractTo generate a hepatitis E virus (HEV) genotype 3 (HEV-3)–specific monoclonal antibody (mAb), the Escherichia coli –expressed carboxy-terminal...
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Protein–protein interaction prediction methods: from docking-based to AI-based approaches
Protein–protein interactions (PPIs), such as protein–protein inhibitor, antibody–antigen complex, and supercomplexes play diverse and important roles...
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Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
High quality, well-validated antibodies are needed to mitigate irreproducibility and clarify conflicting data in science. We describe an...
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Camelization of a murine single-domain antibody against aflatoxin B1 and its antigen-binding analysis
Aflatoxin B 1 (AFB 1 ), a highly toxic mycotoxin, always contaminated in a variety of agricultural products. Camelid variable domain of heavy chain...
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Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry
Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein–protein interaction is the binding...
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Solution NMR Analysis of O-Glycopeptide–Antibody Interaction
O-Linked glycans potentially play a functional role in cellular recognition events. Recent structural analyses suggest that O-glycosylation can be a...
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Binding Efficacy of Heavy Chain Antibody Against Porcine Alpha Amylase Derived from Indian Camel (Camelus dromedarius)
Single domain antibody has huge application in the field of biological sciences. Though many studies have been done on camelid antibody generated...
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Alpha-enolase (ENO1), identified as an antigen to monoclonal antibody 12C7, promotes the self-renewal and malignant phenotype of lung cancer stem cells by AMPK/mTOR pathway
BackgroundTumor-associated antigens (TAAs) can be targeted in cancer therapy. We previously identified a monoclonal antibody (mAb) 12C7, which...
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Antigen–Antibody Complexes
InAntigen vertebrates, immunoglobulins (Igs), commonly known as antibodiesAntibody, play an integral role in the armamentarium of immune...
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Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen
The coupling of surface plasmon resonance imaging (SPRi) with mass spectrometry (MS) offers a very promising multidimensional analysis. This system...
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Homology Modeling of Antibody Variable Regions: Methods and Applications
Adaptive immunity specifically protects us from antigenic challenges. Antibodies are key effector proteins of adaptive immunity, and they are...
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Interaction of pRb and β-Catenin in Cancer and Normal Human Prostate Tissue
AbstractProstate cancer (PCa) is one of the most common oncological diseases, which passes through two stages in its development: localized PCa and...
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NanoBERTa-ASP: predicting nanobody paratope based on a pretrained RoBERTa model
BackgroundNanobodies, also known as VHH or single-domain antibodies, are unique antibody fragments derived solely from heavy chains. They offer...
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Hyper Acute Rejection (HAR)
Hyper acute rejection (HAR) occurs 48 h immediately after transplantation. HAR is caused by cytotoxic antibody against donor’s HLA or ABO antigen....