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Rapid detection of porcine sapelovirus by reverse transcription recombinase polymerase amplification assay
BackgroundPorcine Sapelovirus (PSV) infection has been confirmed in pigs worldwide, mostly asymptomatic, but in some cases, it can lead to...
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Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns
AbstractCongenital cytomegalovirus (CMV) infection is a common cause of sensorineural hearing loss and neurodevelopmental impairment in newborns....
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Development of a loop-mediated isothermal amplification (LAMP) assay for rapid visual detection of snakehead vesiculovirus (SHVV) in snakehead
Infections caused by snakehead vesiculovirus (SHVV) have seen frequent outbreaks in recent years, inflicting significant losses on the snakehead...
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Development of reverse transcriptase-recombinase polymerase amplification (RT-RPA) assay for rapid detection of large cardamom chirke virus
Large cardamom chirke virus (LCCV) causing chirke disease of large cardamom is a major production constraint of this crop. Rapid and accurate...
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Novel multiplex recombinase polymerase amplification assay for simultaneous detection and seroty** of Streptococcus parauberis
Stre pto coc cus parauberis , a gram-positive bacterium, causes significant mortality in cultured fish. In Korea, S. parauberis exhibits diverse...
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Seed amplification assay for the detection of pathologic alpha-synuclein aggregates in cerebrospinal fluid
Misfolded alpha-synuclein (αSyn) aggregates are a hallmark event in Parkinson’s disease (PD) and other synucleinopathies. Recently, αSyn seed...
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Novel helix loop-mediated isothermal amplification (HAMP) assay for colorimetric detection of Staphylococcus aureus in milk
Staphylococcus aureus is an important and leading cause of foodborne diseases worldwide. Prompt detection and recall of contaminated foods are...
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Development of Simplified Recombinase Polymerase Amplification Assay for Rapid and Robust Detection of Citrus Yellow Vein Clearing Virus
Citrus is an economically important fruit crop, belongs to family Rutaceae, cultivated commercially in over 130 countries, which holds a leading...
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Trouble-free detection of grapevine leafroll-associated virus-3 employing reverse transcription-recombinase polymerase amplification assay
Grapevine leafroll-associated virus-3 (GLRaV-3) is a viral pathogen of grapevine leafroll disease (GLD) complex, responsible for debilitating...
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Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis
Syphilis is a re-emerging sexually transmitted disease caused by the pathogenic spirochete T. pallidum. Every year more than 5 million cases are...
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Rapid screening of point mutations by mismatch amplification mutation assay PCR
AbstractMetabolic engineering frequently makes use of point mutation and saturation mutation library creation. At present, sequencing is the only...
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Advancing Nematode Identification on Potato: An Isothermal Recombinase Polymerase Amplification Assay for Stubby Root Nematode, Paratrichodorus allius
The stubby root nematode, Paratrichodorus allius , is an important plant-parasitic nematode that feeds on plant roots and transmits Tobacco rattle virus ...
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A recombinase polymerase amplification–SYBR Green I assay for the rapid and visual detection of Brucella
Brucellosis is a zoonosis caused by Brucella , which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important...
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Development of an isothermal reverse transcriptase-recombinase polymerase amplification assay for the rapid diagnosis of four viroids in different grape cultivars
Rapid, isothermal detection is possible using recombinase polymerase amplification assay (RPA) offering great specificity plus sensitivity. An RPA...
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Rapid and sensitive detection of Taura syndrome virus (TSV) in shrimp based on an isothermal enzymatic recombinase amplification (ERA) assay
Taura syndrome in shrimp, caused by Taura syndrome virus (TSV), is a disease listed by the World Organisation for Animal Health (OIE) that has been...
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A Simplified Isothermal Recombinase Polymerase Amplification Assay for Detection of Sugarcane bacilliform virus Infection
Sugarcane bacilliform virus (SCBV), a circular double stranded DNA genome, is considered as one of the economically important pathogens of sugarcane,...
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Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Detection of Candidiasis Due to Candida parapsilosis
Candida parapsilosis is a common cause of candidiasis among hospitalized patients, often surpassing Candida albicans . Due to the recent increase in C....
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The development of a loop-mediated isothermal amplification (LAMP) assay to detect American foulbrood in managed honey bee populations
Paenibacillus larvae (American foulbrood) is a lethal and arguably the most destructive and economically important notifiable bacterial disease that...
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Rapid Detection of Enterobacter cloacae With a Visualized Isothermal Recombinase Polymerase Amplification Assay
Enterobacter cloacae exhibits strong adhesion and invasion properties that contribute its ability to infect the host; it is considered an important...
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Genetic identification of Staphylococcus aureus isolates from cultured milk samples of bovine mastitis using isothermal amplification with CRISPR/Cas12a-based molecular assay
Bovine mastitis, a common and costly disease in dairy cattle, is primarily caused by Staphylococcus aureus . Timely and accurate detection of this...