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  1. Rapid detection of porcine sapelovirus by reverse transcription recombinase polymerase amplification assay

    Background

    Porcine Sapelovirus (PSV) infection has been confirmed in pigs worldwide, mostly asymptomatic, but in some cases, it can lead to...

    Ramandeep Kaur, Sushila Maan, ... Aman Kumar in Molecular Biology Reports
    Article 22 January 2024
  2. Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns

    Abstract

    Congenital cytomegalovirus (CMV) infection is a common cause of sensorineural hearing loss and neurodevelopmental impairment in newborns....

    Hyeonseek Park, Doo Ri Kim, ... Minhee Kang in Applied Microbiology and Biotechnology
    Article Open access 19 September 2023
  3. Development of a loop-mediated isothermal amplification (LAMP) assay for rapid visual detection of snakehead vesiculovirus (SHVV) in snakehead

    Infections caused by snakehead vesiculovirus (SHVV) have seen frequent outbreaks in recent years, inflicting significant losses on the snakehead...

    Mengya Guo, Zicheng Zhou, ... **aodan Liu in Fisheries Science
    Article 28 February 2024
  4. Development of reverse transcriptase-recombinase polymerase amplification (RT-RPA) assay for rapid detection of large cardamom chirke virus

    Large cardamom chirke virus (LCCV) causing chirke disease of large cardamom is a major production constraint of this crop. Rapid and accurate...

    P. Malavika, A. I. Bhat, M. Greeshma in VirusDisease
    Article 15 April 2024
  5. Novel multiplex recombinase polymerase amplification assay for simultaneous detection and seroty** of Streptococcus parauberis

    Stre pto coc cus parauberis , a gram-positive bacterium, causes significant mortality in cultured fish. In Korea, S. parauberis exhibits diverse...

    Ji Yeong Choi, Guk Hyun Kim, Kwang Il Kim in Aquaculture International
    Article 03 July 2024
  6. Seed amplification assay for the detection of pathologic alpha-synuclein aggregates in cerebrospinal fluid

    Misfolded alpha-synuclein (αSyn) aggregates are a hallmark event in Parkinson’s disease (PD) and other synucleinopathies. Recently, αSyn seed...

    Luis Concha-Marambio, Sandra Pritzkow, ... Claudio Soto in Nature Protocols
    Article 18 January 2023
  7. Novel helix loop-mediated isothermal amplification (HAMP) assay for colorimetric detection of Staphylococcus aureus in milk

    Staphylococcus aureus is an important and leading cause of foodborne diseases worldwide. Prompt detection and recall of contaminated foods are...

    A. Arun Prince Milton, M.C.B. Prasad, ... Sandeep Ghatak in World Journal of Microbiology and Biotechnology
    Article 15 November 2023
  8. Development of Simplified Recombinase Polymerase Amplification Assay for Rapid and Robust Detection of Citrus Yellow Vein Clearing Virus

    Citrus is an economically important fruit crop, belongs to family Rutaceae, cultivated commercially in over 130 countries, which holds a leading...

    Nitika Gupta, Rakesh Kumar, ... Virendra Kumar Baranwal in Current Microbiology
    Article 22 February 2024
  9. Trouble-free detection of grapevine leafroll-associated virus-3 employing reverse transcription-recombinase polymerase amplification assay

    Grapevine leafroll-associated virus-3 (GLRaV-3) is a viral pathogen of grapevine leafroll disease (GLD) complex, responsible for debilitating...

    Gopi Kishan, Rakesh Kumar, ... Virendra Kumar Baranwal in Journal of Plant Diseases and Protection
    Article 20 August 2023
  10. Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis

    Syphilis is a re-emerging sexually transmitted disease caused by the pathogenic spirochete T. pallidum. Every year more than 5 million cases are...

    Krishnamoorthy Priya, Elangovan Saranya, ... Mohandass Ramya in Indian Journal of Microbiology
    Article 22 May 2024
  11. Rapid screening of point mutations by mismatch amplification mutation assay PCR

    Abstract

    Metabolic engineering frequently makes use of point mutation and saturation mutation library creation. At present, sequencing is the only...

    Feng Zhang, Zhen Yang Liu, ... Jian Zhong Xu in Applied Microbiology and Biotechnology
    Article Open access 02 February 2024
  12. Advancing Nematode Identification on Potato: An Isothermal Recombinase Polymerase Amplification Assay for Stubby Root Nematode, Paratrichodorus allius

    The stubby root nematode, Paratrichodorus allius , is an important plant-parasitic nematode that feeds on plant roots and transmits Tobacco rattle virus ...

    Mankanwal Goraya, Gui** Yan, ... Kylie Swisher Grimm in American Journal of Potato Research
    Article 29 December 2023
  13. A recombinase polymerase amplification–SYBR Green I assay for the rapid and visual detection of Brucella

    Brucellosis is a zoonosis caused by Brucella , which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important...

    Jiang Chang, Nan Wang, ... Hong-Lin Ren in Folia Microbiologica
    Article 02 December 2023
  14. Development of an isothermal reverse transcriptase-recombinase polymerase amplification assay for the rapid diagnosis of four viroids in different grape cultivars

    Rapid, isothermal detection is possible using recombinase polymerase amplification assay (RPA) offering great specificity plus sensitivity. An RPA...

    Pankhuri Singhal, Damini Diksha, ... Virendra Kumar Baranwal in Indian Phytopathology
    Article 09 May 2024
  15. Rapid and sensitive detection of Taura syndrome virus (TSV) in shrimp based on an isothermal enzymatic recombinase amplification (ERA) assay

    Taura syndrome in shrimp, caused by Taura syndrome virus (TSV), is a disease listed by the World Organisation for Animal Health (OIE) that has been...

    Jiaobing Li, **gjie Hu, ... Mengqiang Wang in Aquaculture International
    Article 13 March 2024
  16. A Simplified Isothermal Recombinase Polymerase Amplification Assay for Detection of Sugarcane bacilliform virus Infection

    Sugarcane bacilliform virus (SCBV), a circular double stranded DNA genome, is considered as one of the economically important pathogens of sugarcane,...

    B. Parameswari, S. K. Sharma, ... K. Anitha in Sugar Tech
    Article 19 April 2023
  17. Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Detection of Candidiasis Due to Candida parapsilosis

    Candida parapsilosis is a common cause of candidiasis among hospitalized patients, often surpassing Candida albicans . Due to the recent increase in C....

    Bo Zhu, Lei Wang, ... Lei Zhang in Current Microbiology
    Article 18 May 2023
  18. The development of a loop-mediated isothermal amplification (LAMP) assay to detect American foulbrood in managed honey bee populations

    Paenibacillus larvae (American foulbrood) is a lethal and arguably the most destructive and economically important notifiable bacterial disease that...

    Danielle Ackerly, Lily Tran, Travis Beddoe in Apidologie
    Article Open access 11 June 2024
  19. Rapid Detection of Enterobacter cloacae With a Visualized Isothermal Recombinase Polymerase Amplification Assay

    Enterobacter cloacae exhibits strong adhesion and invasion properties that contribute its ability to infect the host; it is considered an important...

    Juan Hu, **g Xu, ... Wenjun Zhu in Current Microbiology
    Article 05 June 2023
  20. Genetic identification of Staphylococcus aureus isolates from cultured milk samples of bovine mastitis using isothermal amplification with CRISPR/Cas12a-based molecular assay

    Bovine mastitis, a common and costly disease in dairy cattle, is primarily caused by Staphylococcus aureus . Timely and accurate detection of this...

    Meruyert Amanzholova, Aisha Shaizadinova, ... Sailau Abeldenov in Veterinary Research Communications
    Article 06 September 2023
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